核苷酸酶

In 1984, Kirkman and Gaetani (1984) reported tightly bound NADPH in BLC, which then was also detected in the X-ray structure of BLC (Fita and Rossmann, 1985a). In the course of various sequencing projects a broad spectrum of catalase sequences from organisms of all kingdoms appeared in the public databases in the last 17 years e.g.

Vainshtein等获得了在3.5A 低等真核细胞中的过氧化氢酶的第一个电子云图，同时Eventoff 也得到了在2.5A处，用X-ray详细分析附面层控制图，1984年Kirkman 和报道了 Gaetani BLC中的紧密结合的还原型烟酰胺腺嘌呤二核苷酸磷酸

Single Nucleotide Polymorphisms of CAD gene among individuals of loblolly pine, one of the most important paper industrial species in southern United States, were rapidly detected with satisfaction using Wave DNA Fragment Analysis System produced by Transgenomic Company lately.

该研究以美国南部重要的造纸工业用材树种火炬松为材料，利用Transgenomic公司最近推出的WaveR DNA片段分析系统，快速检测肉桂醇脱氢酶基因在个体间的单核苷酸多态性，获得了令人满意的结果。

Methods MHC-DRB1, DQA1, DQB1 were examined by using polymerase chain reaction/sequence specific oligonuleotide probe methods in 19 patients with miliary tuberculosis, 36 with tuberculous menigitis,20 with primary tuberculosis, 28 with tuberculous pleurisy and 91 controls.

采用聚合酶链式反应-序列特异性寡核苷酸探针斑点杂交方法，测定原发性肺结核20例、结核性胸膜炎28例、粟粒型肺结核19例、结核性脑膜炎36例和91例正常对照组的MHC-DRB1，DQA1，DQB1等位基因。

In the past ten years, studies have revealed that CRISPs were also widely distributed in venoms of some reptile and mollusc and could block Ca(superscript 2+), K(superscript +) and cyclic nucleotide-gated ion channels.

近十几年来的研究发现，CRISPs还广泛存在于一些爬行动物、软体动物的毒液中，有钾、钙离子通道和环核苷酸门控通道阻断活性及前体加工酶等活性。

In the past ten years,studies have revealed that CRISPs were also widely distributed in venoms of some reptile and mollusc and could block Ca2+,K+ and cyclic nucleotide-gated ion channels.

近十几年来的研究发现，CRISPs还广泛存在于一些爬行动物、软体动物的毒液中，有钾、钙离子通道和环核苷酸门控通道阻断活性及前体加工酶等活性。

Reduced nicotinamide nucleotides can function as reductants to nitrogenase.

还原的烟酰胺核苷酸对固态酶可以起还原剂的作用。

Methods SNPs of IL-6 and IL-18 genes were detected in 120 patients with RA and 168 normal subjects using polymerase chain reaction with sequence-specific primers.

应用序列特异性引物－聚合酶链反应技术检测168名健康体检者和120例RA患者的IL-6基因启动子－572和－174及IL-18基因启动子－607C/A和－137G/C单核苷酸多态性。

In this experiment, we screen the major protective antigen gene-SOD gene of M. paratuberculosisin order to study the sensitive, specific diagnostic reagent and prophylaxis preparation, especially theDNA vaccine. The SOD gene was amplified from Mycobacterium paratuberculosis C-2 chromosomalDNA by using the PCR technique and cloned into pMD18-T Vector System. We gained a SOD gene of624bp.The recombinant clone was identified byα-complementarity, enzyme digestion and PCRidentification. The result indicated that the recombinant plasmid pMD18-T-SOD was successfullyconstructed. Moreover, through sequential determination and DNASTAR analysis between the clonedSOD gene of M. paratuberculosis C-2 and that of the M.paratuberculosis K-10 strain, the sequentialhomogeneity reached 99%, and the amino acid homogeneity reached 99.5%. The preceding analysisindicated that the SOD gene was very conservative in M. paratuberculosis.

为了研制副结核病敏感、特异的诊断试剂和新型、高效的预防制剂，尤其是DNA疫苗，本研究筛选了M.paratuberculosis主要保护性抗原SOD基因，以M.paratuberculosis C-2染色体DNA为模板，以SOD基因的特异性引物进行PCR扩增，获得了624bp的SOD基因，通过T-A克隆技术，将PCR产物克隆至pMD18-T Vector中，以质粒大小、酶切分析、PCR扩增及序列分析鉴定重组克隆，成功地构建出克隆质粒pMD18-T-SOD，序列测定及DNASTAR分析表明，所获得的M.paratuberculosis C-2 SOD基因与Gen Bank中M.paratuberculosis K-10 SOD基因的大小完全一致，两者核苷酸序列的同源性为99％，氨基酸序列的同源性为99.5％，表明该基因在副结核分枝杆菌中是高度保守的。

DNaseⅠ,preferentially attacking double-stranded DNA to produce oligonucleotides with 5'-phosphoryl and 3'-hydroxy termini, is considered to play a major role in digestion of dietary DNA.

目前已发现DNA酶Ⅰ存在6种编码蛋白的基因多态性、单核苷酸多态性和内含子4的可变串联重复序列。

E. Coli polynucleotide phosphorylase was isolated and purified with a purification factor of 68 times.

本文的第一部分报导了从大肠杆菌中分离多核苷酸■酸化酶的工作。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。