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核苷酸酶

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Nicotinamide adenine dinucleotide phosphate oxidase is a major source of reactive oxygen species production in many nonphagocytic cells.

还原型烟酰胺腺嘌呤二核苷酸磷酸氧化酶是许多非吞噬细胞中反应性氧自由基产生的主要来源。

Methods We used retrograde CTb immunocytochemical and NADPH-d histochemical staining methods after microinjecting CTb into cortical amygdaloid.

采用霍乱毒素B亚单位逆行追踪和还原型尼克酰胺腺嘌呤二核苷酸脱氢酶组织化学双重染色相结合的方法。

Magna populations in edge and central region was studied. In 39 A. magna samples and five A. chukar outgroups, the entire mtDNA D loop was PCR amplified. The 456-457 nucleotides of the DNA D loop domain Ⅰ have been sequenced by dideoxy chain termination method. The 16 variable sites(3 5% of the entire sequence) defined 15 haplotypes.

本研究采用聚合酶链式反应和直接测序的方法获得了采自甘肃的大石鸡一个边缘种群和两个中心地理种群共39个个体的线粒体DNA控制区基因456~457个核苷酸的基因序列,16个变异位点(占整个序列的3.5%)有15个单倍型。

The eyes were periodically examined by retinoscopy, and A-scan ultrasonography. Two weeks later, the eyeballs were removed and the apoptotic cells were determined by electron microscopy, TUNEL technique and flow cytometry in scleras. Both bFGF and the bcl-2 protein were determined by the immunohistochemisty method and the Western-blot method. Results: All eyes treated with -10.00D or -20.00D concave lens produced defocus-induced myopia.

实验前后用睫状肌麻痹下验光、A超动态观察豚鼠眼屈光度和眼轴长度,并取后极部巩膜组织,用电镜、终末脱氧核糖核苷酸转移酶介导原位缺口末端标记法和流式细胞术检测巩膜的凋亡细胞,应用免疫组织化学染色和蛋白印迹(Western-blot)法检测bFGF和bcl-2蛋白。

The qualitative and quantitative analysis of schizophrenia and single nucleotide polymorphism of catechol-O-methyltransferase , Proline Dehydrogenase and DYSBINDIN genes were done by using transmission disequilibrium test . Results:(1) The symptoms of first-episode schizophrenia were divided into four independent symptom dimensions: psychomotor poverty, reality distortion, disorganisation and depression-anxiety.

4采用传递不平衡检验、单倍型传递不平衡检验和定量性状传递不平衡检验的方法对6号、22号染色体上3个候选基因,即儿茶酚氧位甲基转移酶(catechol-O-methyltransferase,COMT)、脯氨酸脱氢酶(Proline Dehydrogenase,PRODH)和DYSBINDIN基因的9个单核苷酸多态性(single nucleotide polymorphism,SNP)位点与精神分裂症、神经认知功能、人格特质和临床症状表现的关系进行了定性和定量的遗传学分析。

Objective To explore the relationship between genetic polymorphisms of the ethanol metabolizing enzymes and the occurrence of alcoholic liver disease.

目的利用基因芯片检测乙醇代谢相关酶的单核苷酸多态性,并阐明乙醇代谢相关酶基因多态性与酒精性肝病的关系。

Selecting the better amplified from Diacylglycerol acyltransferase, gene, Acyl- desaturase gene ,then cloning sequencing, afer that, through the NCBI and nucleotide sequence to match homology , the results show that: cloning by the cassava, castor-oil plant Jatropha curcas DGAT and SAD genes and gene fragments have the high homology with other known plant DGAT genes and SAD genes .

从中筛选出扩增效果较好的二酰基甘油酰基转移酶(Diacylglycerol acyltransferase, DGAT)基因,硬脂酰-酰基载体蛋白脱饱和酶(Acyl- desaturase, SAD)基因克隆测序,经测序,通过NCBI与已知的核苷酸序列进行同源性比对,结果表明:克隆所获得的木薯、蓖麻和麻疯树 DGAT基因和SAD基因的片段与其它已知植物的DGAT基因和SAD基因具有很高的同源性。

The prestent invention discloses the cDNA sequnce of a new human reduced NADH: ubiquinone oxidoreductase 14 KDa subunit (NADH-CoQ14KDa subunit). The protein coded with the sequence is the homolog of ox's NADH-CoQB14.5b subunit.

本发明提供了一种新的人还原型烟酰胺腺嘌呤二核苷酸-辅酶Q氧化还原酶14千道尔顿亚基(NADH:ubiquinone oxidoreductase 14 kDa subunit,简称为&NADH-CoQ 14 kDa亚基&的cDNA序列,该序列编码的蛋白是牛NADH-CoQB14.5b亚基的同系物。

To investigate the relationship between the angiotensinconverting enzyme insertion/deletion mutation and the aldosterone synthase (CYP11B2) gene C(-344)T single nucleotide polymorphism in pedigreed primary hypertension and pedigreed nomortension.

目的探讨原发性高血压家系与血压正常家系血管紧张素转换酶基因插入/缺失位点及醛固酮合成酶(CYP11B2)基因C(344)T位点单核苷酸多态性的关系。

Sanger, F., et al. 1980. Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing. J. Mol. Biol.

Sanger酶学法的原理[2]即用双脱氧核苷酸作为链终止试剂,通过聚合酶的引物延伸产生一系列大小不同的分子后再进行分离的方法。

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