核苷酸酶

AIM: To study the effect of acyl coenzyme A:cholesteryl acyltransferase 1(ACAT1) antisense oligonucleotides on the formation of foam cells.

目的：观察酰基辅酶A：胆固醇酰基转移酶1(ACAT1)反义寡核苷酸对泡沫细胞形成的影响。

The results show that all the hmgr genes from pigs, cattle and chickens are astable enzyme proteins with the same gene sequence characteristics and encoding protein and physical and chemical properties; all the amino acid sequences have a high degree of homology, containing two conservative HMG-CoA binding motifs and two NADPH binding motifs; all the amino acid sequences, containing signal peptide, are secreted proteins; they are all hydrophobic transmembrane proteins with five transmembrane domains; random coil and α-helix are the main structural elements in the HMGR polypeptide chain; all the predictions of three-dimensional structure are V- shaped; there are more closer evolution relationship between the HMGR gene from pigs and cattle.

结果表明：猪、牛和鸡HMGR基因序列特点及编码蛋白质理化性质基本相同，均属不稳定类酶蛋白；保守结构域氨基酸序列具有高度的同源性，均含有保守的2个β-羟基-β-甲基戊二酸单酰辅酶A结合基序和2个还原性烟酰胺腺嘌呤二核苷酸结合基序；均有信号肽，属分泌型蛋白质；属于跨膜的疏水性蛋白，均有5个跨膜结构域；无规则卷曲和α-螺旋是HMGR多肽链中的主要结构元件；三维结构预测都呈"V"字形；猪和牛HMGR基因的进化关系最近。

Objective To study the effect of morphine on gene expression of enzymes involved in salvage and catabolism pathways of purine nucleotides metabolism in nerve cells.

目的：研究吗啡对神经细胞模型PC12嘌呤核苷酸补救合成酶与分解代谢关键酶基因表达的影响。

Normal rats were used as control. Immunohistochemical method was used for the expression of iNOS at different times. The iNOS level was also measured by colorimeter and the retinal apoptotic cells were stained by TUNEL method.

在烧伤后不同时间点以免疫组化检测iNOS在视网膜的表达，以酶法检测视网膜组织中iNOS含量的变化，并以末端脱氧核苷酸转移酶介导的dUTP缺口末端标记法检测视网膜凋亡细胞，并与正常大鼠相对照。

Aiming at above problem, this paper put forward the strategy of separation as follows. Firstly, in view of great amount of coloring matter in solution, the quality of product is declined. So we decolor the solution by acticarbon, which makes 80% coloring matter be removed, but the loss of nucleotides is only about 2%. Secondly, on the basis of physical and chemical properties of four nucleotides, we first synthesized a kind of cation exchange resin by ourselves which is used to separate four matters preliminarily.

针对以上问题，本文提出了如下的分离策略：首先，由于酶解液中色素含量较多，使经树脂分离所得UMP和GMP中色素残留较多，最终导致成品质量的下降，所以我们用活性炭对酶解液进行脱色处理，使溶液中80％以上的色素被吸附除去，而核苷酸的吸附损失仅为2％左右。

In order to get purified purified commercially luciferase, we selected whole gene fragment of Photinus pyralis as well as gene fragment deleting 18-nucleotide at 5'termination, which was synthesized according to P〓 template.

为了能得到商品纯的虫荧光素酶，将取自P〓质粒中的全长的北美萤火虫荧光素酶基因片断，及以P〓为模板合成的5＇端缺失18个核苷酸的虫荧光素酶基因片断。

The sequence analysis show that the AlMV-Ch replicase gene consistes of 2373 nucleotides, codes 790 amino acid, and 3'-end un-coding reagin consistes of 166 nucleotides.

结果表明：AlMV-Ch复制酶基因由2373个核苷酸组成，编码790个氨基酸，3′端非编码区由166个核苷酸组成。

Dinucleotide A compound of two nucleotides linked by their phosphate groups .important examples are the coenzymes NAD and FAD.

二核苷酸：由磷酸基团连接的两个核苷酸组成的化合物。例如辅酶 NAD 和 FAD 。

For example, the single-stranded RNAs of retroviruses have about 10,000 nucleotides and the QβRNA is only 4,220 nucleotides long.

举个例子，单链RNA的逆转录酶病毒有大约10，000个核苷酸，而QβRNA只有4，220个核苷酸长度。

For example, the single- strand ed RNAs of retroviruses have about 10,000 nucleotides and the QβRNA is only 4,220 nucleotides long.

举个例子，单链RNA的逆转录酶病毒有大约10，000个核苷酸，而QβRNA只有4，220个核苷酸长度。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。