核苷酸酶

Results: Enzyme digestion and sequence determination and analysis confirmed that the Construction of recombinant plasmid was correct.

结果：酶切鉴定及核苷酸序列测定表明重组质粒的构建完全正确。

The functional allele at the W locus is Ipmyb2, which was newly found in Chinese populations, and it differs mainly in the intron regions from those of the reported allele Ipmyb1; in comparison to Ipmyb2, the newly found second allele ipmyb2 has eight substitutions in the exons, a 6-bp deletion, and a 19-bp deletion, causing ORF shifting, and exists mostly in white-flowered individuals.

以上结果表明：1花青素代谢途径上酶基因的位点均有一定程度的多态，其中尤以ANS为最高；调控基因ipmyb2的核苷酸缺失造成该基因停止功能，使其纯合子的个体开白色花。2授粉者所介导的花色表型选择确实在中国种群中发生，有可能进一步增加花色代谢途径上基因位点的遗传变异。

Cloning and sequencing of the amplifications in 15 cases of Chinese blood donors and dialysis patients were carrited out.

用聚合酶链式反应扩增TTV DNA序列核苷酸1915到2185之间的片段，并将该片段克隆入pGEM-T Easy载体。

The expression of HO-1 was assayed by immunohistochemistry. In situ terminal deoxynucleotidyl transferase assay was used to observe the numbers of brain apoptosis.

以免疫组化法检测HO-1的表达，原位末端脱氧核苷酸转移酶标记法观察脑细胞凋亡细胞数。

Thedideoxynucleotides act as chain terminators since they have no 3'-OHgroup on the deoxyribose which is needed by the synthesis step.

双脱氧核苷酸在脱氧核糖上没有聚合酶延伸链所需要的3'－OH基团，所以可被用作链终止试剂。从得到的PAGE胶上可以读出我们需要的序列。

The dideoxynucleotides act as chain terminators since they have no 3'-OH group on the deoxyribose which is needed by the synthesis step.

双脱氧核苷酸在脱氧核糖上没有聚合酶延伸链所需要的3'－ OH 基团，所以可被用作链终止试剂。从得到的 PAGE 胶上可以读出我们需要的序列。

Objective: The aim of this study was to investigate the effects of ginsenoside Rg3 and ginsenoside Rg3 combined with cisplatinon the growth of nude mice cervical cancer, and to explore the possible mechanism of ginsenoside Rg3 and ginsenoside Rg3 combined with cisplatinin cells and molecules levels. The growth of human cervical cancer Hela cell, apoptosis of tumor cells , expression of CD31and PeNt were observed with optic microscope, radio-immunity immunological histo chemistry, terminal deoxynucleotidy transferase-mediated dUTP nick end labeling, and immunohistochemical staining. The purpose of this study is to provide a new way of medication to eure cervical cancer in clinic.

目的：本实验通过建立子宫颈癌裸鼠病理模型后，以此为研究对象，运用光镜、免疫组化及末端脱氧核苷酸转移酶介导的dUTP缺口末端标记技术，观察人参皂甙Rg3[20—Ginsenoside Rg3]和顺铂单独及二者联合应用对人宫颈癌Hela细胞裸鼠移植瘤生长的影响，重点研究肿瘤的生长、肿瘤细胞的凋亡及PCNA、CD31的表达情况，以期从细胞及分子水平揭示人参皂甙Rg3和顺铂单独及二者联合应用对人宫颈癌Hela细胞的作用机制，为临床上药物治疗与预防宫颈癌寻找更加完善的途径。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。