核苷酸

The nad4 sequences of six cestodes samples(MM11JT,TH29CS,TPW32CZ,TPW33CZ, TH45XX,TPW45) were obtained and their sequence length was 523bp.The six nad4 sequences comparison revealed that they separated into three population.

获得6个代表性样品(MM11JT，TH29CS，TPW32CZ，TPW33CZ，TH45XX，TPW45)的核苷酸序列，序列长度均为523bp，经核苷酸序列互对比较，结果显示它们存在三个种群，且其种间差异为2.1%～30.1%，比种内变异(2.2%)大。

Dinucleotide A compound of two nucleotides linked by their phosphate groups .important examples are the coenzymes NAD and FAD.

二核苷酸：由磷酸基团连接的两个核苷酸组成的化合物。例如辅酶 NAD 和 FAD 。

Sequences were aligned by BLAST method. Results showed that 309bp fragments shared high similarity with orfl38 in Ogu CMS radish and Brassica cybrids of Ogu CMS radish, which 172 nucleotide sequences and 58 amino acids were same among them. And 689bp fragments shared high similarity (100%) with Pol orf224 in B.napus, which 677 nucleotide sequences and 225 amino acids were same among them.

同源性分析结果表明，利用orf138引物所获得的309bP大白菜mtDNA特异片段均与萝卜Ogu CMS、甘蓝型油菜Ogu CMS萝卜体细胞杂种所具有的Ogu orf138高度同源，二者有172个核苷酸完全相同，有58个氨基酸完全相同；orf224引物所获得的689bP大白菜mtDNA特异片段与甘蓝型油菜的Pol orf224高度同源，二者有677个核苷酸完全相同，有225个氨基酸完全相同，同源性均达到100%。

Reverse transcription polymerase chain reaction and western blot were used to detect the COCH and POU3F4/brn4 gene's expression. To investigate in vitro characteristics of COCH gene's expession, two factors were introduced in the culture medium of spiral ligament type Ⅰ fibrocytes respectively. Firstly, phosphorothionate-modified antisense oligodeoxynucleiotides to POU3F4/brn4 mRNA was applied to study the regulation effects of POU3F4/brn4 to COCH gene transcription.

引入人工合成的针对POU3F4／brn4基因mRNA的反义硫代磷酸寡聚脱氧核苷酸，干扰耳蜗螺旋韧带Ⅰ型成纤维细胞POU3F4／brn4的表达，免疫印迹检测反义干扰细胞POU3F4／brn4蛋白条带的强弱，RT-PCR检测反义寡核苷酸干扰细胞COCH基因转录水平的变化，观察POU3F4／brn4对COCH基因的转录调控作用。

Polymorphism of HLA-DQB1 promoter region in Hans IDDM patients and normal controls have been identified by PCR, PCR/SSCP and PCR/sequencing methods.No differences were found in y and s box between patients and controls carrying different allele as well as in different ethnic groups. There are two different sequences in x box,but CCTAGAGACAGATT sequence locates frequently on the haplotype with DQB1.0302 allele. Polymorphism between transcription point and y box (at position －44～-46 and －59～-61) might be associated with the genetic susceptibility to IDDM. Additionally,a new single base mutant (CACC→CAC A ) was found at position －131 and －128 in two patients carrying DQB1.0601 allele.

结果显示携带不同等位基因的患者与对照者DQB1 5'-调控区y、s box核苷酸序列相同，且与白种人基因结构一致；y box核苷酸序列存在二种结构，CCTAGAGACAGATT序列常常与DQB1.0302等位基因在同一单倍型；转录起始位点至y box间-44至-61位存在多态性，-59至-61位AAG等位基因可能与1-型糖尿病易感相关联；在2例携带DQB1.0601等位基因患者的-131至-128位间发现CACC→ACA A单个碱基取代突变。

CONCLUSION摘要: The supplementation of nucleotides can accelerate the repair of damaged DNA in mouse thymocytes in vitro.

结论摘要：外源核苷酸能明显促进受损小鼠胸腺细胞DNA的修复，且核苷酸的功能和其添加水平有关。

The supplementation of nucleotides can accelerate the repair of damaged DNA in mouse thymocytes in vitro.

外源核苷酸能明显促进受损小鼠胸腺细胞DNA的修复，且核苷酸的作用与其添加水平有关。

Rimers for PCR were designed based on nucleotide sequences of glycoprotein B genes of EHV 1 and EHV 4 to amplify specific regions for EHV 1 or EHV 4 or a common region of both viruses.

CR引物是根据编码EHV 1和EHV 4的糖蛋白B基因上的共有的核苷酸序列或型特有的核苷酸序列设计的。

Chemnitzi and Pteria penguin using PCR technique. The PCR products were directly sequenced, and 439 bp nucleotide sequences were obtained after excluding the primers and truncating partial sequences at the end.

chemnitzi和企鹅珍珠贝的16S rRNA基因片段，PCR产物直接测序，删去引物及部分端部序列后，得到439 bp可供分析的核苷酸片段，用MEGA 3.1软件分析了核苷酸差异。

Together with the cited sequences of other species (cattle, zebu cattle and buffalo), the molecular phylogenetic tree was therefore built to probe phylogenetic relationship between Bovine subfamilies, Total 5 variation sites were examined and two types of mutation, transition and transversion, were observed, with the overage percentage of nucleotide variation of 1.66%. It demonstrated the poverty of polymorphism at exon 5 of GH gene of three bovine species. There was only one missense mutation out of 5 mutation sites which led to the shift between leu and val amino.

结果表明，在雷琼牛、巴州牦牛和巴州蒙古牛中共检测到5个变异位点，核苷酸取代方式仅有转换和颠换，核苷酸平均突变率仅为1.66%，说明这3个牛种GH基因外显子5多态性较为贫乏；5个变异位点中仅有1个为错义突变，导致亮氨酸和缬氨酸的转换，但该突变在牛种中分布有差异。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。