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Methods Th multi-epitope peptide was made up of 5 Th epitopes(heat shock protein 65 of Mycobacterium tuberculosis p1-20,E2 protein of Rubella virus p54-65,engineering epitope PADRE,heat shock protein 60 of Chlamydia trachomatis p35-48,and tetanus toxin p830-843),and its gene sequence including of 219bp.

方法复合T辅助细胞表位由5个T辅助细胞表位组成(结核杆菌热休克蛋白65p120、风疹蛋白E2-4p5465、人工合成T辅助细胞表位PADRE、沙眼衣原体热休克蛋白60p3548、破伤风类毒素p830843),其基因序列由219个核苷酸组成。

The Small-tailed Han sheep(48), Ningxia Tan sheep(121), Tan SH (23), Poll Dorset(48), Suffolk(24) were used to study the single nucleotide polymorphisms of H-FABP gene by PCR-SSCP and DNA sequencing. Five nucleotide variations were found, of which G981A, A1014C, and T1019C resulted in amino acid changes of R327H, E338A, and S340P, respectively.

以小尾寒羊(48只)、宁夏滩羊(121只)、滩寒杂交羊F1(23只)、无角陶塞特(48只)、萨福克(24只)5个绵羊群体为实验材料,利用PCR-SSCP和DNA测序技术对心脏型脂肪酸结合蛋白基因(GenBank登录号: AY157617)外显子2和内含子2部分序列进行单核苷酸多态性检测及遗传多态性分析。

They tracked the SORL1 genes ia single nucleotide polymorphisms, or SNPs, which are single-letter changes in a gene's sequence.

研究人员通过基因序列的单节变异-单核苷酸多态性追踪SORL1基因。

Objective To study the effects of bcr/abl antisense oligodeoxynucleotide on K562 cell growth.

目的:研究bcr/abl基因的反义寡聚脱氧核苷酸(antisense oligodeoxynucleotide,ASON)对K562细胞生长的影响。

YAO Jun-Feng, ZHANG Ying, WU Gui-Qin, ZHENG Jiang-Xia, DENG Xue-Mei, YANG NingCollege of Animal Science and Technology, China Agricultural University, Beijing 100094, ChinaSeven single nucleotide polymorphisms were identified by PCR-SSCP and sequencing in the chicken apoA5 gene in F2 chickens from an experimental cross of White Plymouth Rock × Silkies.

以丝羽乌骨鸡和隐性白洛克正反交产生的F2代为实验群体,采用PCR-SSCP和DNA测序的方法检测鸡载脂蛋白A5(apoA5)基因的单核苷酸多态性,并将所发现的SNPs与体重、胸肌重、腿肌重、心脏重、肝脏重和腹脂重等屠体性状进行关联分析。

Cloning and sequencing of the amplifications in 15 cases of Chinese blood donors and dialysis patients were carrited out.

用聚合酶链式反应扩增TTV DNA序列核苷酸1915到2185之间的片段,并将该片段克隆入pGEM-T Easy载体。

Results Five of the PCR amplifications were discovered and sequenced with 300 bp ...

结果对 5个阳性标本的 30 0bp部分M基因组片段序列分析表明均为SEO型汉坦病毒;毒株核苷酸序列比较表明:与其他SEO型毒株同源性均达 94%以上,而与其他HNT型毒株同源性均低于 72 %;毒株序列所构建的系统发生树显示,北京地区SEO型汉坦病毒可能至少存在 4个不同的进化分支和两个不同的汉坦病毒亚型。

American biochemists Horry set of alanyl-transfer RNA 76 nucleotide sequence and in the role of protein synthesis.

美国生物化学家霍利确定了丙氨酰转移核糖核酸的76个核苷酸的顺序及在蛋白质合成中的作用。

The expression of HO-1 was assayed by immunohistochemistry. In situ terminal deoxynucleotidyl transferase assay was used to observe the numbers of brain apoptosis.

以免疫组化法检测HO-1的表达,原位末端脱氧核苷酸转移酶标记法观察脑细胞凋亡细胞数。

Objective To investigate the effects of pentylenetetrazol and nuclear factor kappa B decoy oligodeoxynucleotides on the expression of synaptophysin in neuron-like PC12 cells and explore the epileptogenesis.

目的 通过观测戊四氮(pentylenetetrazol,PTZ)及NP-кB圈套寡核苷酸结构对神经元样PC12细胞突触素(synaptophysin,P38)表达的影响,进一步探讨癫痫的发病机制。

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呼气,收缩臀部肌肉;拱起身体,尽量抬起头来,右腿伸直朝向天花板(膝微屈,以避免肌肉紧张)。

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