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The existence of isonucleoside in oligonucleotide makes chain twist and decreased the stability of duplex.

结论寡核苷酸中的异核苷使链发生扭曲,从而导致双链稳定性降低。

The total RNA of viruses isolated was extracted from the Hantavirus-infected Vero-E6 cells and the S-segment cDNA of Hantavirus was obtained by RT-PCR. This gene fragment was then cloned into plasmid vector pUCm-T and sequenced afterwards by using the DNA STAR software for comparison.

提取病毒总RNA,应用逆转录聚合酶链反应扩增病毒S基因全片段,克隆入质粒载体,进行核苷酸序列测定及分析,应用DNA STAR软件分析比较,确定病毒型别。

The SNPs were genotyped by homogenous mass extend assay in nondiabetic control population.

根据所发现的单核苷酸多态性,通过同源引物延伸质谱分析法对非糖尿病对照人群进行基因分型。

According to the conserved regions of other known insects, a pair of specific primers were designed and the RT-PCR method was used to amplify the partial sequences of HSP70 from cotton aphid, then analyzed the nucleotide and deduced amino acid sequence.

根据已知昆虫HSP70基因的保守性区域,设计特异性引物,采用RT-PCR方法扩增棉蚜HSP70基因的部分序列,测序后进行核苷酸和推导的氨基酸序列分析。

Methods: Four hantavirus strains, which were positive by antigen immunofluorescence assay, were analyzed by reverse transcription polymerase chain reaction, nucleotide sequence and cell culture.

采用逆转录-聚合酶链反应、核苷酸序列测定和细胞培养方法对免疫荧光抗原阳性的4份鼠肺标本进行汉坦病毒分析。

576 sera samples of drug user were collected, and HCV antibodies were detected by ELISA for those samples.

山西吸毒者HCV 1b型分离株在其核心区的144个核苷酸序列中,与日本、河北、上海、湖南及邻省的同源性都在94%以上,与湖南株的同源性最好(97%);而推导的氨基酸序列与日本的差异最大(7%)。

Objective To investigate the effects of CpG oligodeoxyribonucleotide as adjuvants on cellular immune responses in mice immunized with hepatitis B virus gene vaccines.

目的 探讨人工合成的含CpG基序的寡核苷酸作为佐剂对乙型肝炎病毒基因疫苗诱导小鼠产生细胞禁忌免疫应答的影响。

Methods The model of medically intractable epilepsy was established by kindling of amygdale.

建立慢性杏仁核点燃癫痫模型,筛选出耐药组和有效组,通过侧脑室注射全硫代XIAP反义寡核苷酸,对照组分别注射生理盐水。

The fixation of CO2 carried out by these autotrophs, in addition to ATP, requires reduced pyridine nucleotide

除ATP外,由这些自养生物进行的CO2固定,需要还原的吡啶核苷酸

Athletes' selection and cardiopulmonary function training for endurance program will benefit from searching and confirming gene loci of SNP related with maximal oxygen consumption. The formulation and evaluation of exercise prescription for cardi...

寻找并确认影响最大摄氧量的基因位点及单核苷酸多态性对耐力项目的科学选材及心肺功能的合理训练具有重要价值,对心血管疾病运动处方的制定和评价也有一定的参考价值。

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However, as the name(read-only memory)implies, CD disks cannot be written onorchanged in any way.

然而,正如其名字所指出的那样,CD盘不能写,也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口,替代木托盘,免薰蒸,符合欧盟、北美各国对出口货物包装材料的法令要求;喷涂钢托盘适用于重载上货架之用,托盘表面根据需要制作成平板状、波纹状及间隔形式,满足不同的使用要求。

A single payment file can be uploaded from an ERP system to effect all pan-China RMB payments and overseas payments in all currencies.

付款指令文件可从您的 ERP 系统上传到我们的电子银行系统来只是国内及对海外各种币种付款。