核苷酸

The glucose-6-phosphate dehydrogenase increased during overw intering,indicating that reduced condition of cell might keep the cell in pr oper function even in cold temperature,and also for cryoprotectant synthesis.The changes in trehalase activity showed that low activity was for keeping higher tre halose content and high activity for removing it in the spring.

越冬期间，体内葡萄糖-6-磷酸脱氢酶活性增高所产生的还原型烟酰胺腺嘌呤二核苷酸磷酸，可为细胞在亚低温状态下发挥正常功能以及体内抗冻保护性物质的合成提供还原动力，同时通过调节体内海藻糖酶活性来维持越冬期间较高含量的海藻糖和移除春季体内累积的过多的海藻糖。

Methods Three primers, including4,5 and M13 core sequence (5'-GAGGGTG～GCGGTTCT-3'), were used to distinguish variations among 20 clinical isolates of T. rubrum and Trichophyton mentagrophytes.

方法采用寡核苷酸重复序列4，5及M13中心序列(5'-GAGGGTGGCGGTTCT-3')等3种引物，扩增 20株红色毛癣菌和须癣毛癣菌临床分离株的DNA，观察产物电泳带型的差异。

Purpose To investigate the effect of 5'-nucleotidase from Trimeresurus albolabris venom on platelet aggregations and its mechanism.

目的 研究白唇竹叶青蛇毒5'-核苷酸酶对血小板聚集的影响，探讨其作用机制。

In this study, nonionic surfactant sorbitan trioleate (Span 85) was modified with Cibacron Blue F-3GA to become an affinity surfactant (CB-Span 85) and to form affinity-based reversed micelles while dissolved in hexane. Since the CB molecule possesses a specific conformation that mimics nicotinamide adenine dinucleotide, it can bind strongly and specifically to a wide range of nucleotide-dependent enzymes such as dehydrogenases and kinases. Moreover, high protein transfer efficiency in the reverse micelles can be easily achieved by controlling the ionic strength of the aqueous phase.

本实验使用非离子型界面活性剂Span 85与蓝色染料分子Cibacron Blue F-3GA结合，形成具有亲和性的界面活性剂 CB-Span 85，由於CB染料分子具有与NAD+相似的结构，可与含核苷酸部位的蛋白质具有亲和力，将CB-Span 85溶於有机溶剂形成反微胞相，可有效的提高反微胞的选择率，可以只控制离子强度来调节蛋白质进出反微胞的效率。

BMD of lumbar spines and femoral neck, Ward and trochanteric areas were measured by dual energy Xray absorptiometry.

结果 73例绝经后骨质疏松症妇女和61例绝经后正常妇女OPG启动子扩增区中找到两个单核苷酸点突变多态性位点，G209A和T245G。

Site-directed mutagenesis was used to change the gene encoding tryptic bovine liver microsomal hepatic Cyt 〓.

本论文采用寡聚核苷酸定点突变技术对人工合成的编码牛肝细胞色素〓的基因进行改变，得到了目标突变基因。

Leptin is the hormone product of the leptin gene in the adipose tissue. The association of the leptin single nucleotide polymorphisms with uberty, milk yield, SCC, milk protein percent and so on, had been confirmed.

Leptin是leptin基因在脂肪组织表达的一种蛋白质，已证明该基因单核苷酸多态性与奶牛的繁殖力、产奶量、体细胞计数、乳蛋白率等性状相关。

In this study, a systematic analysis of the gene expression pattern between CpG oligodeoxynucleotide-elicited and -unelicited prawn Macrobranchium rosenbergii was undertaken.

本研究以CpG寡核苷酸诱发淡水长脚大虾后，针对诱发和未诱发两组的虾子血球之基因表现模式作系统性分析。

Na-K-Exchanging ATPase is a universal cell membrane protein in higher eukaryotic cells which mediates the anti-concentration gradient exchange of intracellular Na+ for extracellular K+. It plays essential metabolic roles such as maintaining sodium and potassium ion gradients across the plasma membrane. The targeted sequences were amplified by PCR to determine the genome structure of the hithertofore unsequenced portion of the alpha 1 subunit of the human Na-KExchanging ATPase gene which encodes 80-130 aa of its extracellular domain using two different templates, human genomic DNA and a human muscle cDNA library. The PCR products were analyzed by restriction endonuclease digestion and then cloned into a plasmid vector for chemiluminescence sequencing and further analysis.

Na-K-Exchanging ATPase是一种普遍存在于高等生物体内的细胞膜蛋白，主要参与介导K+和Na+在细胞内外之间的逆浓度梯度的转运，并维持一定细胞内外的离子梯度，我们采用聚合酶链式反应方法分别以人基因组DNA及cDNA文库为模板对人Na-K-Exchanging ATPaseα1亚单位基因胞外区约80-130位氨基酸编码序列进行扩增，限制性酶切分析扩增产物，并进行荧光测序，对测序结果进行同源性分析及剪接位点的搜索并对得到的核苷酸序列进行进一步的分析，发现人基因组DNA和cDNA经过扩增后分别得到833bp和195bp两种不同大小的片段Fg，Fc。

All illustrate that NF-κB ASODN is effect to the rat endometriosis model, further verificate there is intimate relation between NF-κB and endometriosis.

说明用NF-κB反义寡核苷酸抑制治疗内异症模型有效，进一步证实NF-κB与内异症的发病机制有密切关系。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷