核苷酸

In order to study species resource from ruminal fluid, one strain of methionine- degrading bacterium MB6-1 was isolated and purified. 16 S rRNA encoding gene (16 S rDNA) from MB6-1 was amplified by PCR, and its nucleotide sequence was determined.

摘 要：为研究山羊瘤胃液中甲硫氨酸降解菌群的物种资源，对经分离纯化获得的一株甲硫氨酸降解菌MB6-1，采用PCR方法扩增其16 S rDNA基因，并测定其基因的核苷酸全序列。

The screening method of seven alleles (Z-6,Z-4,Z -2,Z,Z+2,Z+4 and Z+6) in then dinucleotide tandem repeat sequence was st udied. These alleles constitute a microsatellite DNA marker upstream of the tran scription initiation site on the aldose reductase gene.

湖南医科大学学报000103 摘要：研究了醛糖还原酶基因转录起始点上游-2.1kb处的微卫星DNA标记--n二核苷酸串联重复序列7种等位基因(Z+6，Z+4，Z+2，Z，Z-2，Z-4和Z-6)的筛选方法。

The screening method of seven alleles (Z-6,Z-4,Z -2,Z,Z+2,Z+4 and Z+6) in then dinucleotide tandem repeat sequence was st udied. These alleles constitute a microsatellite DNA marker upstream of the tran scription initiation site on the aldose reductase gene.

研究了醛糖还原酶基因转录起始点上游-2.1kb处的微卫星DNA标记--n二核苷酸串联重复序列7种等位基因(Z+6，Z+4，Z+2，Z，Z-2，Z-4和Z-6)的筛选方法。

Identification of cripto-1 as a novel serologic marker for breast and colon cancer［J］.

脂质体介导的cripto反义寡核苷酸抑制结肠癌细胞端粒酶活性［J］。

Was serologically related to both PenMV and SCMV-BJ.

所得的核苷酸序列与SCMV-BJ的同源性达到99.28%。

Methods 5-FU, MTX, CDDP in different concentrations were given to every group after transfected with Cyclin D1 SOND or ASODN for 24 h ,the do~-effects response was observed and 1C50 was calculated in every group.

在给予Cyclin D1 ASODN和Cyclin D1反义寡核苷酸转染细胞24h后，分别给予梯度浓度的5-氟尿嘧啶（5-FU）、氨甲喋呤、顺铂，观察各组的量效反应，计算IC50。

The survival rates of CEM cells cultured with cis-diamminedichicloroplatinum added 24 h later were higher than that cultured with hTERT ASODN and DDP added 24 h later. The survival rates of CEM cells cultured with DDP were similar with that cultured with hTERT SOND and DDP. In morphological observation of apoptotic cells using Giemsa staining, cells treated with DDP or DDP combined with hTERT ASODN ro SODN at 48 h, displayed classic apoptotic changes. Apoptosis rates of CEM cells treated with DDP for 48 h after 24 h of exposure to ASODN significantly increased. There was significant difference in the percentage of apoptotic cells of CEM cells between hTERT ASODN plus DDP and SODN plus DDP or DDP alone, respectively.

结果： hTERT ASODN作用于CEM细胞24 h再加入柔红霉素、长春新碱、足叶乙甙，对细胞生长的抑制分别与单用柔红霉素、长春新碱、足叶乙甙及hTERT正义寡核苷酸联合柔红霉素、长春新碱、足叶乙甙组相比，统计学上无显著差异(P＞0.05)。hTERT反义核酸作用于CEM细胞24 h加入顺铂，再共同作用48 h，CEM活细胞均数为2.318×108 cells/L，与单用顺铂组(3.250×108 cells/L)及hTERT正义核酸联用顺铂组(3.175×108 cells/L)相比，对细胞抑制明显增强(P＜0.05)。hTERT ASODN作用于CEM细胞24 h再加入顺铂作用48 h，细胞出现典型的凋亡形态学改变。hTERT ASODN与2.5 μmol/L顺铂联合作用于CEM细胞48 h的凋亡细胞百分率(19.47%)分别同SODN与顺铂联合作用组(6.97%)、单用顺铂作用组(6.02%)进行比较有显著差异(P＜0.01)。

SsAPX showed the highest homology to the APX gene of Spinacia oleracea, with 84% identity in nucleotide sequence and 89% identity in deduced amino acid sequence.

BLAST同源性分析表明，该cDNA与已报告的菠菜细胞质抗坏血酸过氧化物酶基因同源性最高，在核苷酸水平上一致性为 87%，在氨基酸水平上一致性为 89%。

Hereditary spinocerebellar ataxia Trinucleotide repeat Gene diagnosis

遗传性脊髓小脑型共济失调；三核苷酸重复；基因诊断

Objective: To explore the mechanisms resulting in the recurrence of urethral scar which make urethral strictures difficult to be cured, a series experiments were conducted to find potential effective factors involved in urethral scar formation and degradation, including the studies of extracellular matrix component of urethral stricture scar, the characteristics of urethral scar fibroblast, and the effects of urine on urethral fibroblast in vitro, as well as the studies to compare the difference of collagenase activity, type Ⅰ collagen and tissue inhibitor of metalloproteinase-1 (TIMP-1) in the tissues and cultured fibroblasts from normal urethra and strictured urethra respectively, and the studies to investigate the effect of antisense TIMP-1 oligodeoxyonucleotide on cell proliferation and collagenase activity of urethral scar fibroblast.

中文题名尿道瘢痕基础研究副题名胶原酶活性，TIMP-1的表达及其反义基因治疗外文题名 Experimental study on urethral scar-activity of collagenase，expression of TIMP-1，and antisense TIMP-1 gene transfection of urethral scar fibroblast 论文作者黄翔导师杨宇如教授学科专业外科学研究领域\研究方向学位级别博士学位授予单位四川大学学位授予日期2002 论文页码总数104页关键词尿道手术瘢痕胶原酶成纤维细胞尿道瘢痕馆藏号BSLW /2003 /R699 /12 目的：研究尿道瘢痕的细胞外基质的组成，尿道瘢痕成纤维细胞的生物学特性以及尿液对其生长的影响；比较胶原酶活性，金属蛋白酶组织抑制因子-1（TIMP-1）以及Ⅰ型胶原含量在尿道瘢痕和正常尿道组织及体外培养的成纤维细胞中的差异；研究反义TIMP-1寡核苷酸对尿道瘢痕成纤维细胞增殖以及胶原酶活性的影响。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷