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核苷酸

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The CDS of the obtained bovine ANGPTL1 gene shows 91%,90%,82%, and 93% identity with the corresponding, human, chimpanzee, rattus and dog, respectively.

DNA序列的G+C百分含量为44.31%,A+T百分含量为55.69%。该基因的核苷酸序列与人、黑猩猩、鼠和狗ANGPTL1基因的cDNA序列的相似性分别为91%、90%、82%和93%。

Meanwhile, comparison of sequence between AlMV-Ch与AlMV425 show that the homology of the nucleotide and deduced amino acid sequences of the replicase are 97.8% and 97.6% respectively, but the homology of 3'-end un-coding reagin is 98.2%.

AlMV-Ch与AlMV425进行了同源性比较,结果表明复制酶基因编码区核苷酸序列同源性为97.8%,而推测的氨基酸序列同源性为97.6%,3′非编码区的同源性为98.2%。

Methods: Total RNA was extracted from esophageal carcinoma tissue, the primer containing special enzyme was designed, NY-ESO-1 fragment was amplified by RT-PCR, and then was cloned into the expression vector pET-15b by LP Recco PCR Cloning. The recombinants plasmid pET-15b-NY-ESO-1 were identified by restriction endonucleases digest analysis and sequence analysis.

从人新鲜的食管癌组织中提取总RNA,通过RT-PCR技术扩增NY-ES0-1基因3'端的340bp片段,采用靶向克隆法将目的基因插入到原核表达载体pET-15b中,得到重组表达质粒pET-15b-NY-ESO-1,经过筛选,挑选出阳性克隆进行XhoⅠ和BamHⅠ双酶切图谱分析、PCR检测和扩增产物核苷酸序列分析等,鉴定所构建的原核表达载体。

Methods: Use MLT(micro lymphocytetotoxicity test) and the SSOPH method detect HLA-A, B, and DRB1 antigen to 1,572 cord blood, some samples has question with PCR-SSP and the SBT method to redetect.

采用单克隆板和寡核苷酸探针杂交方法对15 72份脐血检测HLA -A、B、DRB1抗原,用PCR -SSP及SBT方法对有疑问的结果进行复核。

RNA editing is a process to remodify the mRNA coding the genes and the enzyme which has the modifying function is called RNA editing enzyme.

RNA编辑是DNA转录成RNA后, RNA编辑酶对前体mRNA中的核苷酸进行删除、添加或重新修饰的过程。

For obtaining maximal yield of hydrogen how to make glycolysis to produce large amount of NADH at TCA cycle and also to reoxidize FADH2 through electron transport chain by oxygen or other electron acceptors were chief introduced .

文中着重介绍了如何通过 TCA cycle使葡萄糖酵解产生大量的 NADH(还原型二磷酸吡啶核苷酸),抑制 NADH脱氢酶络合物的电子迁移链而得到最高氢气产量。

A mononucleotide,C10H14N5O7P,found in animal cells and reversibly convertible to ADP and ATP;adenosine monophosphate.

一磷酸腺苷单核苷酸,C10H14N5O7P,发现于动物细胞中并能可逆地转化为二磷酸腺苷和三磷酸腺苷;一磷酸腺苷

All sequences were aligned with S genes in GeneBank by BLAST software.The result indicated that all 10 fragments contained domain encoding 5 high conservation regions(C1,C2,C3,RC4,C5) and hypervariable regionof rosaceous S-RNase.RHV and intron characterized S gene furthermore.

测序结果表明,这些DNA片段的核苷酸序列中都具有编码蔷薇科S-RNAase 5个高度保守区域:C1、C2、C3、RC4、C5的序列、编码高变区的序列以及变异度很大的内含子序列,并且其高变区和内含子序列具有扁桃S基因特异性。

Nucleotide-induced eNOS phosphorylation and activity were inhibited by BAPTA-AM (an intracellular free calcium chelator), rottlerin, and protein kinase C siRNA.

BAPTA-AM(一种细胞内游离钙螯合剂),rottlerin和蛋白激酶C siRNA抑制核苷酸介导的内皮型一氧化氮合酶磷酸化和活化。

In order to study species resource from ruminal fluid, one strain of methionine-degrading bacterium MB6-1 was isolated and purified. 16 S rRNA encoding gene (16 S rDN4) from MB6-1 was amplified by PCR, and its nucleotide sequence was determined.

为分析研究山羊瘤胃液中甲硫氨酸降解菌群的物种资源,对经分离纯化获得的一株甲硫氨酸降解菌MB6-1,采用PCR方法扩增其16 S rDNA基因,并测定其基因的核苷酸全序列。

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然而,正如其名字所指出的那样,CD盘不能写,也不能用任何方式改变其内容。

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