核苷酸

Objective To investigate the difference of nucleotide sequence between cholera toxin of O139 and O1 Vibrio cholerae.

目的 探讨O139霍乱弧菌肠毒素核苷酸与O1群霍乱CTX毒素核苷酸序列异同。

Deduced amino acid sequence is highly similar to that of six OBPs from Diptera, with sequence identity of 59%~82%.

MdomOBP3由618个核苷酸组成，拥有从51～515共465个核苷酸的开放阅读框，编码154个氨基酸。

Result showed that dorking CSF gene was 435 bp in size with an ORF encoding 141 amino acids residues, the nucleotide homology between dorking CSF and CSF gene of Gallus, human, canis, Bos taurus, Sus scrofa, sheep, mice was respectively 98.4%%, 28.7%, 29.2%, 33.6%, 30.8%, 29.4% and 34.7%, Amino acids homology was respectively 95.2%, 8.3%, 9.0%, 15.3%, 12.4%, 11.0% and 12.0%.

序列分析表明，CSF的cDNA开放阅读框为435 bp，编码144个氨基酸，与红原锦鸡的序列比较，其核苷酸的同源性为98.4%，氨基酸的同源性为95.2%，同人、犬、印度野牛、野猪、绵羊和老鼠的CSF序列作比较，其核苷酸的同源性分别为28.7%，29.2%，33.6%，30.8%，29.4%和34.7%，氨基酸的同源性分别为8.3%，9.0%，15.3%，12.4%，11.0%和12.0%。

In this paper, we focus on such aspect as mathematic expression in DNA and protein sequences, similarity analysis and phylogenetic tree structure. Mainly include:After summarization mathematical methods of DNA and protein sequences in detail, the paper presents a 2D graphical representation of DNA sequences based on dual nucleotide, and graphical representation of the distribution curve of sequences with nucleotide sequences of the classification.

论文在DNA和蛋白质序列的数学表示、相似性分析和进化树的构造等方面进行研究，主要工作包括：在对DNA和蛋白质序列的数学表示方法进行较为详细的综述后，提出了一种基于双核苷酸的DNA序列2D图形表示方法，利用核苷酸的分类给出了序列的分布曲线图形表示。

Gene mutations were detected in 25 patients and a 18-year-old girl among 16 families. Trinucleotide repeats of CAG were 73～79. The fragments of abnormal alleles were 380～402bp, and all patients were heterozygous. The copy numbers of normal alleles were 18～40, fragments from 202～270bp. SCV reduction was much obvious compared to MCV, MCV and SCV in lower limb ?ere much more slow than that in upper's. BAEP, VEP were also delayed in latency. The anticipation in parental sex bias were much more obvioius than that in matental's. Cerebellar ataxia was most severe, the next were dysarthria and bulging eyes. Amytrophy was seen only in bed ridden patients. Cerebellar atrophy was more severe than brain stem, cord atrophy was n't observed in all MJD.

结果检出10个家系25例病人及1例症状前18岁女孩有MJD基因突变，CAG三核苷酸重复73～79次，异常等位基因片段长380～402bp，均为杂合子；正常人CAG三核苷酸重复18～40次，等位片段长200～270bp，电生理发现MJD的SCV减慢比MCV明显，而下肢的MCV、SCV又较上肢明显，BAEP、VEP均有不同程度的潜伏期延长或波的异常；MJD的父亲遗传早于母亲，进展也较块，临床以小脑性共济失调为突出症状，其次为构音障碍、突眼等，肌肉萎缩仅见于晚期病人；MRI示小脑萎缩较明显，脑干萎缩并不严重，未见明显的颈髓萎缩。

A 717 base pairs long artificial gene fragment of PrV gE has been synthesized by using preferred codons in the highly expressed Pichia genes. Four groups of oligonucleotides were successively cloned in pMD18-T vector to obtain four large fragments.

通过人工合成基因的方法对编码gE蛋白的第29～268位氨基酸残基的核苷酸序列（717bp）进行改造，用P.pastoris高表达蛋白偏爱的密码子替换原有的核苷酸序列，氨基酸序列保持不变。

Mutation rate at exon of GH in five bovine species was very low with the percentage of 3.48%. The majority of nucleotide substitution was nonsense mutation and only one missense mutation was observed. Molecule tree based on haplotypes of bGH at exon 5 showed that differentiation was apparent relatively between Bubalus bubalis and Bos taurus, Bos indicus, Bos grunniens, Bos frontalis. There was no apparent differentiation among other four bovine species and they shared mutual ancestral sequence.

序列分析表明，5个牛种GH基因外显子5的遗传变异水平较低，平均核苷酸变异率约为3.48%，而且绝大多数位点的核苷酸替换是同义突变，仅发现1个错义突变位点；从GH基因外显子5序列单倍型构建的分子进化树来看，水牛与普通牛、瘤牛、牦牛及大额牛的分化相对比较明显，其他4个牛种之间并无明显分化，还享有共同的祖先序列。

This result showed that the nucleotide sequence of the coding region and accordingly amino acid sequences of Myf-5 gene of gayal and cow, chimpanzee, rhesus monkey, human, dog, house mouse, Chinese softshell, chicken and zebrafish were quite conservative.

大额牛与普通牛、黑猩猩、恒河猴、人、狗、家鼠、软体贝壳、鸡、斑马鱼的Myf-5基因编码区核苷酸序列同源性分别为99.0％、90.0％、90.5％、90.5％、87.2％、84.8％、72.9％、64.3％、51.8％，相应的氨基酸序列同源性分别为99.6％、94.9％、94.6％、94.9％、89.9％、88.7％、81.6％、70.4％、56.6％，这说明大额牛与其他9个物种在Myf-5基因的编码区核苷酸和相应氨基酸序列上具有较高的保守性。

It had lowest homology in nucleotide sequence with guinea pig, Callithrix jacchus, which is 83％～84％.

梅花鹿与豚鼠、狨的核苷酸同源性最低，为83％~84％，有57~59个核苷酸不同。

Sequence analysis showed that the coding region of BDNF gene from malayan bear was highly homologous with those of human and giant panda counterpart genes (94% and 99% homology, respectively).

对该基因所作的序列分析表明，马来熊和人BDNF基因编码区的核苷酸序列同源性为94%，而与大熊猫BDNF基因的核苷酸序列同源性高达99%。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。