核苷酸

The papA gene was amplified from the pilus adhesive cluster of UPEC132, and then was cloned into plasmid pUC18. The recombinant plasmid was identified and subjected to DNA sequencing.

采用此引物扩增132菌株p菌毛粘附基因群的papA基因，扩增产物克隆入质粒，选取阳性重组质粒进行核苷酸序列分析。

But thefunctions of SOD-like genes were not approved.We cloned SOD gene fromBombyx mori Nuclear Polyhedrosis Virus genome by PCR.

我们克隆了家蚕杆状病毒的sod基因，测定其核苷酸序列与人源的SOD1基因同源性为56％，其中铜、锌离子的结合位点和酶活性中心位点相当保守。

The results indicated that:The multi\|locus DNA fingerprint with a new synthesized probe\|JL\|02,has enough polymerism and good stability,and should be very useful to monitor the chimerism in different tissues of ES cell chimeric mouse and to check whether an ES cell line has the capacity to enter the germ line,especially when involving strains that can not be discerned with coat color or biochemical markers.

结果表明：采用新型的人工合成的寡核苷酸多聚体探针(JL-02探针)的多位点DNA指纹图谱，具有足够的多态性和很好的稳定性。适用于鉴定ES细胞在嵌合体小鼠各种组织器官中的嵌合情况，也适用于鉴定ES细胞是否具有种系嵌合能力，尤其适用于在嵌合体研究中，最适宜的品种组合具有相同的毛色或相同的酶型。

The results showed that Non-haemagglutinating activity was showed in the isolate. In agar diffusion test,there was a clear precipitin band formation to anti-IBDV specific serum. 28-day-old chicks inoculated with the isolate showed disease,and the isolate was recovered from the tissues of these chickens. The 1 041bp specific fragment were amplified by RT-PCR with sequence-specific primers based on IBDV VP3 gene. The VP3 gene shared 97.7%~98.2% nucleotide identities to very virulent IBDV,97.7%~95.2% nucleotide identities with classical strains from seqiemce analysis.

结果表明，该分离病毒无血凝性，在琼脂扩散试验中能与抗IBDV特异性血清出现1条清晰的白色沉淀线；人工感染28日龄雏鸡出现与临床一致的病变，并回收到病毒；应用针对IBDVVP3基因的特异性引物进行RT-PCR，能扩增到长度为1041bp的特异性目的片段；序列分析发现分离毒VP3基因与IBDV超强毒和经典毒株的核苷酸同源性分别为97.7%~98.2%和95.2%。

In order to test the practical usefulness of the prober LZF-I we conducted population investigation, paternity determination, tissue stability and applicable extent in fish and obtained following results. The probability of chance association between random individuals was 9.23×10-16,and this indicates that the LZF-I fingerprints are completely individualspecific The paternity probability was 0.999962, and it showed that the inheritance of alleles is in accord with the Mendelian Law. The LZF-I oligonucleotide fingerprinting probe was stable.It was very effective in detecting both individual and species specificities of fish.

通过对鱼类的群体实验、亲子鉴定实验、组织细胞的稳定性实验和鱼类种类的适用范围实验后，测得：(1)LZF-I DNA指纹探针属多位点寡核苷酸探针；(2)LZF-I DNA指纹探针在鱼类种群中的鉴别机率为9.23×10 E-16；(3)LZF-I DNA指纹探针在鱼类亲子鉴定实验中的父系概率为0.999962；(4) LZF-I DNA指纹探针，是一种稳定的，既具有个体识别能力，又具有一定种属特异性的、适用于鱼类DNA指纹图研究的基因指纹探针。

90In this paper, the 10kDa sulfur-rich prolamin gene was amplified by PCR, cloned and sequenced from Chi-nese rice. The sequence analysis showed that the amplified fragment was 380bp long, 94. 5 % homologousto the nucleic acid sequence reported by Masumura et al.

对扩增产物的核苷酸序列分析表明：本扩增产物长 380bp；与 Masumura等人[1] 发表的序列相比，有94.5％的同源性，与我们以前发表的中花10号水稻10kDa富硫醇溶蛋白基因的序列相比，有99.5％的同源性。

Rice, 10kDa sulfur-rich prolamin , pseudogene, nucleic acid sequence

中文关键字：水稻，10kDa富硫醇溶蛋白，假基因，核苷酸序列

Furthermore, the genetic relationship between the polymorphism of Prolactin and the prolificacy of Small Tail Han Sheep was explained.

结果表明： 5个绵羊品种在引物2的扩增产物中均存在单核苷酸多态性，其中只有多赛特羊未检测出DD型，而其余4个绵羊品种均检测出CC、CD和DD型。

Pseudorabies virus ; Glycoprotein G ; Nucleotide sequence ; Amino acid sequence ; Gene expressing

伪狂犬病病毒；糖蛋白G ；核苷酸序列；氨基酸序列；基因表达

Objective To investigate the effects of purine nucleotides compensation on pain threshold in heroin dependent rats.

目的 探讨嘌呤核苷酸补偿对海洛因依赖大鼠痛阈的影响。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。