核苷酸

The identities of the pGM-CSF coding sequence when compared to human, bovine, ovine and murine sequences were 82%, 85%,88% and 86%, respectively at the nucleotide level, and 72%, 73%, 73% and 57%, respectively at the amino acid level.

与人、牛、羊、小鼠的 GM-CSF 核苷酸序列的相似性分别为 82%、 85%、 88%和 86%，氨基酸序列相似性分别为 72%、 73%、 73%和 57%。

In this experiment, we screen the major protective antigen gene-SOD gene of M. paratuberculosisin order to study the sensitive, specific diagnostic reagent and prophylaxis preparation, especially theDNA vaccine. The SOD gene was amplified from Mycobacterium paratuberculosis C-2 chromosomalDNA by using the PCR technique and cloned into pMD18-T Vector System. We gained a SOD gene of624bp.The recombinant clone was identified byα-complementarity, enzyme digestion and PCRidentification. The result indicated that the recombinant plasmid pMD18-T-SOD was successfullyconstructed. Moreover, through sequential determination and DNASTAR analysis between the clonedSOD gene of M. paratuberculosis C-2 and that of the M.paratuberculosis K-10 strain, the sequentialhomogeneity reached 99%, and the amino acid homogeneity reached 99.5%. The preceding analysisindicated that the SOD gene was very conservative in M. paratuberculosis.

为了研制副结核病敏感、特异的诊断试剂和新型、高效的预防制剂，尤其是DNA疫苗，本研究筛选了M.paratuberculosis主要保护性抗原SOD基因，以M.paratuberculosis C-2染色体DNA为模板，以SOD基因的特异性引物进行PCR扩增，获得了624bp的SOD基因，通过T-A克隆技术，将PCR产物克隆至pMD18-T Vector中，以质粒大小、酶切分析、PCR扩增及序列分析鉴定重组克隆，成功地构建出克隆质粒pMD18-T-SOD，序列测定及DNASTAR分析表明，所获得的M.paratuberculosis C-2 SOD基因与Gen Bank中M.paratuberculosis K-10 SOD基因的大小完全一致，两者核苷酸序列的同源性为99％，氨基酸序列的同源性为99.5％，表明该基因在副结核分枝杆菌中是高度保守的。

The nucleotide acid sequences of the four fragments had more than 95% homology with Lectin gene (AF001527) and Pectate Lyase gene (x92943) which were submitted to GenBank .

此四条片段的核苷酸序列分别与GenBank中发表的Lectin基因(AF001527)及Pectate Lyase基因(X92943)具有95％以上的同源性。

To scientifically control and prevent Newcastle disease,this study researched the molecular evolution characteristics of Phosphoprotein gene of Newcastle disease viruses.

对1997~2007年国内分离的13个NDV毒株的P基因进行扩增测序，与15个已发表的国内外不同时期的NDV毒株P基因进行核苷酸和推导的氨基酸遗传变异分析及分子特性研究。

Respiratory syncytial virus; Phosphoprotein gene; Sequence analysis

呼吸道合胞病毒； P蛋白；核苷酸序列

DNaseⅠ,preferentially attacking double-stranded DNA to produce oligonucleotides with 5'-phosphoryl and 3'-hydroxy termini, is considered to play a major role in digestion of dietary DNA.

目前已发现DNA酶Ⅰ存在6种编码蛋白的基因多态性、单核苷酸多态性和内含子4的可变串联重复序列。

E. Coli polynucleotide phosphorylase was isolated and purified with a purification factor of 68 times.

本文的第一部分报导了从大肠杆菌中分离多核苷酸■酸化酶的工作。

E.Coli polynucleotide phosphorylase was isolated and purified with a purification factor of 68 times.

本文的第一部分报导了从大肠桿菌中分离多核苷酸砱酸化酶的工作。

The main clime features included ataxia, hypopsia, axanthocyanopsia and retinal pigmental degeneration. Alleles from 7 to 9 repeats were seen in the other 4 healthy members. GAG repeats from 6 to 21 were found in other 126 SCA patients, 71 family members and 60 healthy controls.

该家系内表型正常的4位成员SCA7等位基因CAG重复数目为7~9，另126例临床表现为SCA的患者、71名表型正常的家系成员及60名健康对照者SCA7等位基因内CAG三核苷酸重复数为6~21。

Spinocerebellar ataxia Olivopontocerebellar atrophy Retinal pigmental degeneration Trinucleotide repeat

脊髓小脑性共济失调；橄榄桥脑小脑萎缩；视网膜色素变性；三核苷酸重复

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。