核苷酸

The genomic DNA A of squash leaf curl virus from Chinahas been cloned and its complete nucleotide sequence was determined.The DNA A consisted of 2441 nucleotides.

对引起我国南瓜曲叶病的病毒分离物DNA的克隆和序列分析表明，中国南瓜曲叶病毒DNA A由2741个核苷酸组成。

The vip3A genes amplified from wild-type Bt strains S184 and 611 with different levels of activity against lepidopteran insect larvae were cloned into pGEM-T Easy vector. Alignment of these 2 putative Vip3A proteins with 8 others showed that vip3A genes are highly conservative.

从以上菌株中挑选2个对夜蛾科害虫具有较高和较低毒力的菌株，即S184和611，分别进行vip3A基因的克隆和测序，再已报道的其它8个vip3A基因进行核苷酸和氨基酸序列比较，结果表明，vip3A是一个极其保守的基因。

Based on the combined dataset of nucleotide and amino acid sequences of 13 protein coding genes,using the maximum likelihood and Bayesian inference methods to reconstruct the phylogenetic relationship of fifteen species of lepidopteran insects,The resulst showed the same topologies and supported a close relationship between the Geometroidea,Noctuoidea and Bombycoidea.This result deviates from the traditional view.

基于线粒体基因组13个蛋白质编码基因的核苷酸序列及氨基酸序列的联合数据集构建了15种鳞翅目昆虫的系统树，采用BI和ML分析方法建树，所得拓扑结构类似，都显示蚕蛾总科，尺蠖蛾总科和夜蛾总科关系较近，结果与前人研究的结果不完全一致。

Single Nucleotide Polymorphisms of CAD gene among individuals of loblolly pine, one of the most important paper industrial species in southern United States, were rapidly detected with satisfaction using Wave DNA Fragment Analysis System produced by Transgenomic Company lately.

该研究以美国南部重要的造纸工业用材树种火炬松为材料，利用Transgenomic公司最近推出的WaveR DNA片段分析系统，快速检测肉桂醇脱氢酶基因在个体间的单核苷酸多态性，获得了令人满意的结果。

The occurrences, relative abundance, relative density, most common and longest SSRs in the three species were analyzed and compared with each other. The results revealed that the SSRs number in B. japonicum, M. loti and S. meliloti genomes were 1410, 859 and 638, respectively. In the three genomes, tetra-, penta-and hexa-nucleotide repeats were more abundant, indicating higher mutation rates in these species.

大豆慢生根瘤菌、百脉根根瘤菌和苜蓿中华根瘤菌基因组中的SSRs分别为1410个、859个和638个，3种根瘤菌基因组中长重复的四、五、六核苷酸基序更为丰富，变异性更高。

Low Nucleotide diversity indicated that genetic differentiation coming from the same origin was relatively low, and may be have a recent common ancestry for the populations.

较低的核苷酸多态度表明，同一起源的家鹅mtDNA群体分化程度较低，它们拥有较近的共同祖先，且可能是在不同的时间多个中心由鸿雁野生种驯化而成。

Objective: With umbilic blood from the mature newborns, to screen CpG-ODN, which can markly boost up the immunity response, from some designed CpG-ODNs; to compare the effect of CpG-ODN on the lymphopoiesis of umbilic blood from the premature, mature newborns and the peripheral blood from the adults; to observe the infulence of CpG-ODN on the immune function of the pregnant mices and their newborns, the immune-inhibited and aged mices.

目的：设计含非甲基化胞嘧啶和鸟嘌呤的寡聚脱氧核糖核苷酸，以期用足月新生儿脐血筛选出具有较强免疫增强作用的CpG-ODN；比较CpG-ODN对新生儿脐血和成人外周血单个核细胞的增殖效应；观察CpG-ODN对孕鼠及其新生鼠、免疫抑制鼠和老年鼠体液免疫功能的影响。

To construct the combined site_directed random mutation library of recombinant human Lymphotoxin for in vitro molecular evolution study, and to study the structure and function relationship.

应用含随机核苷酸序列的引物，通过OverlapPCR的方法分别对rhLT的 4 6、10 6和 130位氨基酸进行定点随机突变，获得各单点随机突变体库。

Phage display of recombinant human lymphotoxin mutation librariesand receptor affinity screeningContraction of conformable large molecule mutation library is pivotal for molecular evolution study in vitro. In order to extend the range of the screening region of amino acid in LT gene, several receptor binding sites of LT were mutated randomly by overlap PCR amplification with the random nucleotide primers, the R46, S106, L130 combined site-directed random mutation library and R46~A52, S106~F110, R46~A52 + S106~F110 region random mutation libraries of rhLT were constructed successfully.

二。重组人淋巴毒素噬菌体库的构建及受体亲和筛选构建适合的大容量生物大分子变异体文库是进行体外分子进化研究的关键，为了扩大LT基因序列中被筛选的氨基酸范围，我们采用含随机核苷酸序列的引物，通过Overlap PCR的方法对LT的受体结合区域进行多点随机突变，分别构建了rhLT R46+S106+L130三点随机突变组合文库和R46～A52、S106～F110、R46～A52+S106～F110区域随机突变体库。

AIM: To investigate the effect of antisense oligonucleotides on expression of macrophage migration inhibitory factor in macrophages.

目的：研究巨噬细胞移动抑制因子反义寡核苷酸对体外巨噬细胞表达MIF的影响。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷