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The homology of the nucleotide sequences and genetic variation analysis of NDV strains suggested that mere were obvious divergence in gene lever among NDV vaccine strains and epizootic strains isolated from different areas and different ages.The divergence may have effect on amino acid and structure and function of protein in some extent and lead to difference of immunogenicity ,which was one of reason being difficult in preventing from ND.

通过对NDV F基因核苷酸序列同源性比较及遗传变异分析发现:我国不同年代、不同地区分离的NDV毒株与疫苗毒株之间在基因水平上存在较大差异,这种差异对氨基酸及蛋白质的结构和功能可能会有一定影响,而NDVF蛋白的差异同时也造成免疫原性的差异,这很可能是新城疫的免疫防治十分困难的原因之一。

Objective To observe cataract incidence、characteristics、and conjunction with trinulceotide repeat,n,in the 3,-untranslated region of the myotonin protein kinase gene in twenty-five patients,consistent with typically or equivocally clinical and molecular diagnosis criteria of DM.

摘 要:目的观察强直性肌营养不良临床典型或可疑的患者并符合分子诊断标准的25例患者中白内障发病率、特点及与强直性肌营养不良蛋白激酶基因中CTG三核苷酸(C-胞嘧啶、T-胸腺嘧啶、G-乌嘌呤)重复次数的关系。

Selected the best cultivate condition by cultivated on four different media under different conditions; Observated morphology perperties; Studied on biology perperties by germ tube testing, Carbohydrate fermentation testing, Tween testing and Esculin dissociation; lysis testing.

选择四种不同的培养基分别在不同温度条件下培养,选出最优培养条件;通过光学显微镜、电子显微镜观察菌落及菌体的形态学特点;通过芽管试验、碳水化合物发酵试验、碳水化合物同化试验、沙堡培养基生长试验、过氧化氢酶试验、七叶苷分解试验、吐温试验研究该菌的生理、生化学特征;应用PCR技术通过特异性的引物扩增得到菌属特异性的片段,最后测定扩增片段的核苷酸序列,从而进行菌种的分析、鉴定。

In the second part, we tried to find some SNPs that are related to the etiology of MDS.

本文第二部分,探讨了单核苷酸多态性与MDS发病的关系。

Objective To construct an eukaryotic gene expression vector for CD44v6 and its expression in tumor cells.

目的构建含人的肿瘤侵袭和转移的特异性抗原CD44v6编码基因的真核表达的重组载体,进行核苷酸序列分析,并在肿瘤细胞中表达。

The alignments of the deduced amino acid sequences from eyestalk cDNAs of theshrimps P.chinensis,T.curvirostris,and P.stylirostris and the crab E.sinensis withneuropeptides of CHH family from various crustaceans demonstrate that they have thehighest identity and homology with most MIHs from crustaceans.

根据核苷酸数据和氨基酸数据,比较这四种甲壳动物的MIH以及已发表的六种MIH之间的相似度,同时分析这十种MIH的系统进化关系,结果是蓝对虾、鹰爪虾和中华绒螯蟹的MIH相似性较高,亲缘关系较近,这与物种的系统演化不相符。

With the direction of Professor Zhang Jiawei, basing on the clinic research of treating falling sickness with thread embedding therapy, summing-up many years clinic experience of thread embedding therapy, we observe and compare the two curative effect of thread embedding therapy and general acupuncture in treating chronic gastritis, in addition we study corresponding functionary mechanism with stomach electricity graph、gastrin、nucleotide、SP etc and provide science basis and theory instruction. According to the criterion of diagnosis and elimination set, 70 patients are took into the trial whom come from No. 1 affiliated hospital, among them 36 case are in the thread embedding Group, 34 case are in the acupunture Group.

本课题是在导师张家维教授及李素荷教授等协助导师共同指导下,在既往穴位埋线疗法治疗癫痫临床研究基础上,总结多年来穴位埋线疗法的临床经验,对穴位埋线及穴位针刺两种方法治疗慢性胃炎的疗效进行观察与比较,并拟用胃电图、脑肠肽、环核苷酸、P物质等指标变化来探讨其中的可能治疗机理,为临床的应用及推广提供科学的依据与理论指导。

Genetic diversity and phylogenetic relationship in goldfish were examined using mitochondrial D-Loop partial sequences at eight representative varieties of goldfishes and wild crucian carp. The eight representative varieties were Red Common Goldfish, Red Fantail, White Oranda with Red Carp, White Pearlscale, Red Tigerhead, Black Moor, Red Moor with Butterfly Tail and Red Bubble-eye with Dorsal Fin. 699 base-pair nucleotide sequences of Mitochondrial D-Loop were examined and analyzed for genetic polymorphism, Sequences data showed that all 129 sequences were grouped into 17 haplotypes. Nine representative varieties shared same haplotype. Five representative varieties of goldfishes had haplotypes respectively.

采用PCR技术扩增了红草金鱼,红文鱼,红顶白高头,白珍珠,红狮头,红龙睛蝶尾,墨龙睛,红水泡八个品种金鱼和野生鲫线粒体DNA的D-Loop区部分序列,PCR产物经纯化、测序、同源序列比对后获得长度为699bp的核苷酸序列,在129个样本中共检测到了17个单倍型,9个品种共享1种单倍型,5个品种的金鱼(BM, MB, WR, RF, RC)检测到各自独有的单倍型。

The synthetic oligonucleotide probe5/5 for DNA, fingerprinting was studied in this paper.

本文用自制的寡核苷酸探针5/5,对与法医学应用有关的问题进行了研究。

Riboflavin impurity in synthetic flavin mononucleotide was detected by the proposed method.

最后,将所建立的方法应用于黄素单核苷酸钠合成品中的核黄素杂质的检测,合成品中的核黄素杂质含量约为3%左右,符合产品规格。

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In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是,在否定句或疑问句里,这种带有"do"的方法表达的效果却没有什么强调的意思。

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