核苷酸

The invention further provides methods for binding a DmGPCR, methods for identifying modulators of DmGPCR expression and activity, methods for controlling a population of insects with a DmGPCR antibody, a DmGPCR antisense polynucleotide, a DmGPCR binding partner or modulator, and methods of preventing or treating a disease or condition associated with an ectoparasite.

本发明还提供了结合DmGPCR的方法，鉴定DmGPCR表达和活性的调节剂的方法，用DmGPCR抗体控制昆虫群体的方法，DmGPCR的反义多核苷酸，DmGPCR结合伴侣或调节剂，和预防或治疗与外寄生虫有关的疾病或情况的方法。

The sensitive sites are just the bonds of highest electron affinity and lowest ionization potential. Formation of the sites results from the interaction between several nearby nucleotides. So, the sensitive sites should be a piece of DNA sequence.

敏感位点即这些相对较弱或亲电性较强的化学键，而这种化学键的产生是与敏感位点邻近的几个核苷酸相互作用的结果，即敏感位点应该是一段DNA序列。

The PAL-1 gene promoter defined the nucleotide sequences CTCCAACAACCCCTTC responded to both UV irradiation and elicitor application.

在PAL基因中的类似区域有一个17个核苷酸组成的区域elre参与植物对紫外照射和激发子应答。

RESULTS The chromatographic grade purity of the eluate is above 98% by HPLC,the recovery rate of dCMP,dAMP and dTMP is above 95%,the recover.

结果实验结果发现dCMP，dAMP，dTMP的收率为95%以上，dGMP的收率为85%以上，所获得的混合脱氧单核苷酸经高效液相色谱检测色谱纯达98%以上。

The virus isolate Y322 was obtained from Solarium aculeatissimum showing leaf curl and enation symptoms in Yanshan, Yunnan Province.

从云南砚山刺茄上分离到病毒分离物Y322，全序列测定表明，Y322 DNA-A全长2730个核苷酸，共编码6个ORF。

The virus isolate Y322 was obtained from Solanum aculeatissimum showing leaf curl and enation symptoms in Yanshan,Yunnan Province.

从云南砚山刺茄上分离到病毒分离物Y322，全序列测定表明，Y322DNA-A全长2730个核苷酸，共编码6个ORF。

After thermal induction, no specific recombinant protein band in SDS-PAGE was found, but G-CSF activity was detectable. Therefore, a new recombinant plasmid pBVHG2 expressing hG-CSF hybrid protein with additional 8 amino acids which could be cut off specifically with the help of mucosal enterokinase at the N terminus of hG-CSF was constructed.

含此质粒菌株虽然表达菌体裂解后可测得明显的生物学活性，但SDS-PAGE仍未见特异表达产物带；因此，再应用相同方法，在hG-CSF cDNA突变体5′端增加24核苷酸对的FLAG肽编码序列，构建了hG-CSF杂交蛋白（hG-CSF天然蛋白N末端增加8aa的FLAG肽，后者可由肠激肽酶切除）的表达质粒pBVHG2。

RESULTS:①Enzymolysis and nucleotide sequence analysis of recombinant plasmids showed that two VEGF-C siRNA eukaryotic expression vectors were constructed successfully.

结果：①重组质粒的酶切、核苷酸序列分析结果：经酶切和测序鉴定证实成功构建了血管内皮生长因子C小干扰RNA表达载体，建立了稳定转染细胞株。

Although overexpression of Hspa5 does not cause detectable defects, its knockdown by injecting Hspa5 morpholino antisense oligonucleotides results in slower epiboly during gastrulation and dorsalized phenotypes at 24 hpf, including smaller and curled tail and absence of caudal ventral fin. When Hspa5 expresison in embryos is knockdown, the expression of the dorsal markers gsc and ntl is enhanced while the expression of ventral markers is reduced.

尽管在胚胎中过量表达Hspa5对胚胎发育没有明显影响，但是利用反义吗啉环寡核苷酸抑制胚胎中Hspa5的表达会导致胚胎外包变得缓慢，24hpf出现尾部变细或卷曲、缺少腹鳍等背部化的表型，同时gsc和ntl等背部标记基因的表达增强，但eve1等腹部标记基因的表达减弱。

A genechip analysis was performed using RNAs derived from embryos injected with squint mRNA, MZoep mutant embryos that are deficient in Nodal signaling, and wildtype embryos at the 30% epiboly stage Transcriptswith at least two-fold changes in expression level between wildtype and the other samples were identifyied In squint mRNA-injected embryos, 265 transcripts show anincreased expression level and 111 have a decreased expression level; in MZoep embryos, the expression of 1 495 transcripts increases while 550 transcripts express at a decreased level.

为鉴定受Nodal信号调控的基因，特别是那些转录因子基因，通过将来自squint过量表达、缺失Nodal信号的MZoep突变体或野生型30%外包期胚胎的RNA与Affymetrix斑马鱼寡核苷酸芯片杂交。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷