核苷酸

This is the first crystallization report of the wild-type deoxycytidylate deaminase.

据目前所知，这是第一个关于野生型的脱氧胞嘧啶核苷酸脱氨酶的结晶学报道。

The main regions (405-1 146 nt) in the glyco pro tein gene of 8202,BRV and MRV,isolated from deer,cattle and mouse in China re spectively,of rabies virus was amplified by reverse transcriptase-polymerase ch ain reaction,cloned and sequenced.

对来源于鹿、牛和鼠的狂犬病病毒野毒株82 02、BRV、MRV的G基因405～1 146位核苷酸序列进行了RT-PCR扩增、克隆和序列测定，并应用计算机软件对这3个毒株的测定序列和人源株CGX89的相应序列进行了分析比较。

(1) The high throughput and effective Agilent oligomicroarray can screen novel therapy targeted genes by analyzing the DEGs in development and progression of HCC;(2) The development and progression of HCC is a complicated process involving multigenes and multiprocedures;(3) DKK1, as a novel gene, is involved in the development and progression of HCC and may be a new therapy target.

应用Agilent寡核苷酸芯片高通量、高效率地分析原发性肝细胞癌发生发展过程中的基因表达差异，可以筛选新的治疗靶点；原发性肝细胞癌的发生发展涉及多基因、多步骤，是一个复杂的过程；DKK1可能是原发性肝细胞癌发展过程中的新的分子靶点，其表达变化涉及肿瘤进展。

Generally, B in the configuration closest cell's DNA conformation, it and the double helix model are similar. A-DNA and when RNA member double helix area as well as duplication forms the DNA-RNA hybrid molecule conformation is close. Z-DNA take the nucleotide dimer as the unit left-hand winding, its principal chain assumes the denticle the shape, therefore.

一般认为，B构型最接近细胞中的DNA构象，它与双螺旋模型非常相似。A－DNA与RNA分子中的双螺旋区以及转录时形成的DNA－RNA杂交分子构象接近。Z－DNA以核苷酸二聚体为单元左向缠绕，其主链呈锯齿形，故名。

The members with Leber hereditary opˉtic neuropathy have a point mutation in mtDNA and the mutation at np11778is one of mechanisms inducing this disease.PCR technique provides a useful diagnostic aid for LHON.

该LHON家系成员存在线粒体DNA点突变，mtDNA的ND4亚单位第11778核苷酸突变是本病的发病机制之一，PCR-限制性核酸内切酶酶谱分析技术为LHON的诊断提供了依据。

Cleavage by Dicer creates short double-stranded RNAs that are characterized by two nucleotide long, 3' overhangs.

Dicer切割后会产生一类在3'端有2个核苷酸突出之小段双股RNA，即siRNA，意为「小片段的干扰RNA」。

Methods Surface plasma resonance, circular dichroism and fluorescence experiments were conducted.

应用表面等离子体共振、圆二色散以及荧光光谱法对白蛋白与硫代寡核苷酸的结合行为进行了表征。

Single nucleotide polymorphism is the most common type of sequence difference between alleles, which can be used as a kind of high-throughput genetic marker.

单核苷酸多态性(Single nucleotide polymorphism， SNP)是等位基因间序列差异最为普遍的类型，可作为一种高通量的遗传标记。

Since most of the large molecules containing phosphorus element have double bonds, it is necessary to consider the special structures of double bonds in order to deal with these large systems more accurately. So, based on density functional theory and electronegativity equalization principle, applying a general atom-bond electronegativity equalization method , using separated program, through regression and least-squares optimization , the parameters of atoms and bonds in molecules are determined . By these parameters , the charge distributions and the energy of some molecules containing phosphorus element, especial the fragment of DNA, RNA, are calculated . The results are quite good.

由于这些含磷生物大分子体系都含有双键，为了明确的处理双键，进而更准确的解释带有双键和共轭双键的含磷生物大分子结构与性质的关系，我们以密度泛函理论~([1-4])和电负性均衡原理~([5-9])为基础，应用和开发原子-键电负性均衡方法中的σπ模型，利用最小二乘法，拟合确定了一些分子（含碳、氮、氧、氢以及磷等原子）的各种类型原子及化学键区域的参数，并利用上述参数计算了一些多聚核苷酸的电荷分布以及分子的总能量，进行了讨论，得到的结果可以和从头算相媲美。

Methods Human hepatocarcinom cells lines were transfected with an ectogenesis p53 (Adp53) to observe the action of p53 on wnt signaling pathway by fluorescence-activated cell sorting and RT-PCR.

利用反义寡核苷酸(oligodeoxy nucleotide，ODN)将p53反义ODN导入到人肝癌HepG2(含野生型p53)和人大肠癌Lovo(含野生型p53)细胞株中，观察p53表达受阻后对Wnt通路及FrpHE、DKK-1表达的影响，以进一步明确p53的调节作用。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷