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The inhibin a gene、 the bone morphogenetic protein 4 (BMP4) gene and the bone morphogenetic protein 7 (BMP7) gene were studied as candidate genes on the prolificacy of Small Tail Han sheep. Single nucleotide polymorphisms of 5 ' regulatory region、 exon 1 of INHA gene;exon 2、 exon 3 and exon 4 of BMP4 gene and exon 2、 exon 3 of BMP7 were detected in high fecundity breed and low fecundity breeds (Chinese Merino sheep, Corriedale sheep and South African Mutton Merino sheep) by PCR-SSCP. The results indicated that there was a mutation (316C→T) in 5' region of INHA gene in Small Tail Han sheep, Chinese Merino sheep and Corriedale sheep, the same mutation did not exist in South African Mutton Merino sheep.

采用PCR-SSCP技术检测抑制素α(inhibin α,INHA)基因5'调控区、外显子1,骨形态发生蛋白4(bone morphogenetic protein 4,BMP4)基因外显子2、外显子3和外显子4以及骨形态发生蛋白7(bone morphogenetic protein 7,BMP7)基因外显子2和外显子3在高繁殖力绵羊品种以及低繁殖力绵羊品种(中国美利奴绵羊、考力代绵羊、南非肉用美利奴绵羊)中的单核苷酸多态性,同时研究这三个基因对小尾寒羊高繁殖力的影响。

Immunoprecipitation and cresyl fast violet staining were employed to detect the expression of relevant message proteins, activation and the death of the hippocampal neurons.results it was found that hpk1 was expressed in rat hippocampus neurons.conclusions administration of hpk1 antisense oligodeoxynucleotides can significantly protect hippocampus neurons from apoptosis without dose-dependence.

使用免疫沉淀和焦油紫染色法等技术检测相关信号蛋白的表达、活化水平及神经元细胞的死亡。结果在大鼠海马神经元中有hpk1的表达,脑室注射as-odns大鼠海马ca1区存活锥体细胞明显增多。结论 hpk1反义寡核苷酸对缺血/再灌注引起的海马神经元凋亡具有保护作用。

At last, adsorbent and covalent Ag nanoparticles modified electrodes were prepared by adsorbent and covalent method, respectively. The electrochemical behaviours of SCN-, brilliant cresyl blue and nicotinamide adenine dinucleotide on modified electrode were studied by Cyclic voltammetry, differential pulse voltammograms and electrochemical impedance spectroscopy.

最后,用吸附法和共价键合法制备了吸附型和共价自组装银纳米修饰电极,采用循环伏安法、示差脉冲伏安法和交流阻抗谱等电化学方法研究了SCN-、灿烂甲酚蓝和还原型烟酰胺腺嘌呤二核苷酸的电化学行为。

The origin analysis on the source of hosts and terrains was carried out on the base of homology tree and phylogenetic tree founded in 19 isolates on their CP gene nucleotide sequences. The result showed that the gene types and clusters of Cucumber green mottle mosaic virus were not obviously related to the two factors.

对包括该分离物在内的19个CP基因核苷酸序列进行同源性比较和系统进化树构建,并结合寄主来源及地域来源进行LHP的起源分析,结果表明黄瓜绿斑驳花叶病毒不同分离物的序列分群和进化关系与上述两个因素无明显关系。

Objective:To evaluate the effect of the plasma cyclic nucleotide in patients with pregnancy-in-duced hypertension.

目的:探讨血浆环核苷酸在妊高征中的变化以及作用。

Methods: The distribution of CMPase in the retina of 20-week-old WKY and SHR were studied by electron microscopic cytochemistry.

应用电镜酶细胞化学方法对 20 周龄正常京都种大鼠和同周龄的自发性高血压大鼠视网膜组织的胞嘧啶单核苷酸酶活性部位进行定位观察。

In the last ten years, Chinese scientists have found that angustmycin A had biological activities of cytokinin.

狭霉素A是一种特异的鸟嘌呤核苷酸合成酶抑制剂,在实验室中广泛应用。

Sequence analysis reveals the identity rate of β-actin cDNA nucleotide sequence between the rice field eel and Tilapiamossambica, Salmo salar, Oryzias latipes, Oncorhynchus mykiss, Danio rerio, Carassius auratus, Physalaemus pustulosus, Bos taurus, Gall us gallus, Mus musculus, Hoino sapiens are 68%~95%; and the similarity of 3-actin amino acid sequence is 97%~100%.

将所得序列与各科鱼、蛙、鸡、牛、鼠、人等的β-肌动蛋白基因序列同源性分析显示,核苷酸序列具有68%~95%的相似性,氨基酸序列具有97%~100%相似性,显示该基因在生物进化过程中的保守;系统发育分析表明黄鳝β-肌动蛋白基因与罗非鱼的亲缘关系最近。

DNA is uses by many deaeration nucleotide residue according to certain smooth each other 3 ', 5 '- di-phosphate ester key connected constitution long chain.

DNA是由许多脱氧核苷酸残基按一定顺序彼此用3',5'-磷酸二酯键相连构成的长链。

DNA refers to deoxyribonucleic the acid deoxyribonucleic acid (chromosome and gene constituent) the deaeration nucleotide high polymer, is the chromosome principal constituent.

DNA 指deoxyribonucleic acid 脱氧核糖核酸(染色体和基因的组成部分)脱氧核苷酸的高聚物,是染色体的主要成分。

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