核苷酸

Based on analysis of Wx of D4063-1, indica and japonica and according to the special sites of the three species, primers as markers-AUT4063-I were designed to distinguish D4063-1 from other rice. Combining with primer pair F5, dominant and codominant ways were established for discriminating them, and rapid and correct identification of D4063-1 from other rice could be done.

本研究还根据D4063-1和籼稻、粳稻的序列差异及D4063-1在该片段上的特征序列位点设计了用于识别D4063-1的寡核苷酸片段，并作为PCR反应的引物命名为AUT4063-1，将该引物与作者设计的扩增普通籼稻、粳稻Wx基因的引物F5配合使用，建立了识别D4063-1的显性和共显性两种检测方式的分子标记，为快速、准确鉴别低直链淀粉含量突变体D4063-1创造了条件。

Normal rats were used as control. Immunohistochemical method was used for the expression of iNOS at different times. The iNOS level was also measured by colorimeter and the retinal apoptotic cells were stained by TUNEL method.

在烧伤后不同时间点以免疫组化检测iNOS在视网膜的表达，以酶法检测视网膜组织中iNOS含量的变化，并以末端脱氧核苷酸转移酶介导的dUTP缺口末端标记法检测视网膜凋亡细胞，并与正常大鼠相对照。

Aiming at above problem, this paper put forward the strategy of separation as follows. Firstly, in view of great amount of coloring matter in solution, the quality of product is declined. So we decolor the solution by acticarbon, which makes 80% coloring matter be removed, but the loss of nucleotides is only about 2%. Secondly, on the basis of physical and chemical properties of four nucleotides, we first synthesized a kind of cation exchange resin by ourselves which is used to separate four matters preliminarily.

针对以上问题，本文提出了如下的分离策略：首先，由于酶解液中色素含量较多，使经树脂分离所得UMP和GMP中色素残留较多，最终导致成品质量的下降，所以我们用活性炭对酶解液进行脱色处理，使溶液中80％以上的色素被吸附除去，而核苷酸的吸附损失仅为2％左右。

Pickuping and selecting the characters of control regions, such as sequence similarity, oligos composition, transcription factor binding sites, repeat sequences.

目的 1。提取和选择调控元件模块的特点，如序列相似性、寡核苷酸组成、转录因子结合位点、重复序列等方面的特点。

Objective To investigate the protective effects of mixed nucleotides on liver injury in mice induced by concanavalin A.

目的 探讨混合核苷酸对刀豆蛋白A所致肝损伤的保护作用。

The eyes were periodically examined by retinoscopy, and A-scan ultrasonography. Two weeks later, the eyeballs were removed and the apoptotic cells were determined by electron microscopy, TUNEL technique and flow cytometry in scleras. Both bFGF and the bcl-2 protein were determined by the immunohistochemisty method and the Western-blot method. Results: All eyes treated with -10.00D or -20.00D concave lens produced defocus-induced myopia.

实验前后用睫状肌麻痹下验光、A超动态观察豚鼠眼屈光度和眼轴长度，并取后极部巩膜组织，用电镜、终末脱氧核糖核苷酸转移酶介导原位缺口末端标记法和流式细胞术检测巩膜的凋亡细胞，应用免疫组织化学染色和蛋白印迹（Western-blot）法检测bFGF和bcl-2蛋白。

SSCP was detected in exon 6 and exon9p of the gene, but not in the other exons.Results In polyacrylamide gel electrophoresis, We have not found abnormal conformer in both affected individuals and control cases. There were no differences in the DNA sequence between these two groups.

结果 在非变性聚丙烯酰氨凝胶电泳中，未发现DCM组和对照组该基因的外显子6和外显子9p出现异常构象，通过DNA测序证实两组的核苷酸序列没有差异。

The levels of drought tolerance and nucleotide polymorphism at CBF4 locus in a worldwide sample of 17 core accessions of Arabidopsis thaliana were examined. The results showed that different accessions had quite difference in adaptation to drought stress.

以生长于不同生态气候条件下的17个拟南芥核心生态型为材料，分析了它们的相对抗旱性和抗旱转录因子CBF4 基因的单核苷酸多态性。

The prolactin gene was studied as a candidate gene for the prolificacy of Small Tail Han sheep. Single nucleotide polymorphisms of exon 1 to exon 5 of PRL gene were detected in high fecundity breeds and low fecundity breeds (Dorset, Texel and Corriedale sheep) with five pairs of primers by PCR-SSCP.

设计5对引物，采用PCR-SSCP技术检测催乳素（prolactin，PRL）基因外显子1至外显子5在高繁殖力绵羊品种和低繁殖力绵羊品种（多赛特、特克塞尔和考力代）中的单核苷酸多态性，同时研究该基因对小尾寒羊高繁殖力的影响。

The gonadotropin releasing hormone receptor gene was studied as a candidate gene for the prolificacy of Small Tail Han sheep. Eight pairs of primers were designed to detect single nucleotide polymorphisms of exon 1, exon 2 and exon 3 of GnRHR gene in both high fecundity breeds and low fecundity breeds (South African Mutton Merino, Corriedale and Chinese Merino sheep) by PCR-SSCP. Only the products amplified by primers P4 and P7 displayed polymorphisms.

设计8对引物，应用聚合酶链式反应-单链构象多态性技术检测促性腺激素释放激素受体（gonadotropin releasing hormone receptor， GnRHR）基因外显子1、外显子2和外显子3在高繁殖力品种和低繁殖力品种（南非肉用美利奴、考力代和中国美利奴绵羊）中的单核苷酸多态性，同时研究该基因对小尾寒羊高繁殖力的影响。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。