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Chinaberry witches'broom, Ziziphus jujube witches'broom, Mulberry dwarf disease, JWB and Paulownia witches' broom phytoplasmas were identified by comparative studies of PCR amplify of phytoplasmal 16SrDNA、23rDNA and ribosomal protein gene, heteroduplex mobility assay, RFLP of PCR-amplified products (16S rDNA), cloning and sequence analysis of 16SrDNA and rp gene. An efficient procedure for rapid identification and differentiation of phytoplasmas was established, which could identify and differentiate phytoplasma collected from field.

通过对桑萎缩病、枣疯病、酸枣丛枝病、泡桐丛枝病和苦楝丛枝病植原体16SrDNA、23SrDNA和核糖体蛋白基因的PCR扩增、异源双链迁移率分析、16SrDNA PCR产物的限制性片段多态性分析以及植原体16SrDNA和rp基因的克隆和序列分析等比较研究,建立了一种快速确定未知植原体种类和分类地位的分子鉴别与鉴定优化程序;此程序可对田间采集的各种植物植原体样品直接进行快速鉴定和鉴别。

Using universal primers (R16mF2/Rl and R16F2/R2) for 16S rRNA and primers (rpF1/rpR1) for ribosomal protein gene to detect phytoplasmas associated with of chinaberry tree witches'-broom from Yunnan and Guizhou Province by polymerase chain reaction.

根据植原体16S rRNA基因通用引物序列(R16mF2/R16mRl、R16F2/R16R2)及核糖体蛋白基因通用引物序列(rpF1/rpR1),应用PCR技术对采自不同省份的苦楝丛枝病植原体进行检测,结果从两个植物上分别获得苦楝丛枝病植原体云南株系和贵州株系。

The life-form is divided into two forms,the prokaryotes and the eukaryotes.

再转向核糖核酸,将核糖体的小亚单位,作为区别生命类型之间亲缘关系的指标。

Mitochondria was relatively little in size. Round primary lysosome with high electron-densed granules and secondary lysosome with high or low electron-densed granules were seen frequently. DCs contained many rough endoplasmic reticulum, the Golgi apparatus and ribosomes. The vacuoles with flocculent electron-densed granules were rare. Some special granules in cytoplasm were seen, whose surface like earphone were covered with a membrane. High electron-densed contents in the granules were near one side and the other side was bright. The nucleus became markedly small in volume, nephroid or hoofed in shape. The nucleus had little euchromatin and lots of heterochromatin under nuclear membrane.

子宫内膜癌组织DC超微结构特征如下:细胞形态不规则,与正常子宫内膜组织DC相比,胞膜较光滑,胞膜表面树突状胞浆突起显著减少,部分突起呈粗短状;胞质中线粒体相对少,圆形而电子密度高的初级溶酶体和不规则形且电子密度高低不一的次级溶酶体多见;高尔基体、粗面内质网、核糖体丰富;含微量絮状电子致密物的胞饮小泡显著减少;胞质中可见形态特殊的颗粒,该颗粒外周膜包裹,略呈圆形,中间部位稍弯曲,如耳机状,颗粒中由高电子致密物居于一侧,而另一侧则呈透亮状;胞核显著减小,居于胞质一侧,常呈肾形或马蹄形,核内常染色质较少,异染色质多边集于核膜下。

The ribosome is a complex molecular machine made up of proteins and RNA molecules.

核糖体是由蛋白质和核糖核酸分子组成的复杂分子机器。

The ribosomal RNA gene of silkworm Attacus is found to Be a multi-copy gene with its repeated units arranged tandem.

蓖麻蚕的核糖体核糖核酸的基因是多拷贝基因,其重复单位成线性方向排列。

Objective To explore sequence differentiation of ITS and 28S rDNA-LSU of Orientobilharzia turkestanicum from bovine and caprine hosts. Methods Adult worms of O.

摘要目的探讨牛羊源土耳其斯坦东毕吸虫( Orientobilharzia turkestanicum )核糖体内转录间隔区和28S核糖体大亚基序列的差异。

Sequence analysis showed that the ITS gene was proved to be a complex structural region which was not suitable for the identification of Prototheca species, but the LSU and SSU rDNA regions showed 94﹪ and 99.9﹪ sequence identities with Prototheca zopfii var.

核酸序列分析显示核糖体内转录间区DNA可能存在复杂结构,不适合无绿藻的测序鉴定;但核糖体大亚基及小亚基DNA序列分析,与祖菲无绿藻碳水化合物变种(Prototheca zopfii var.hydrocarbonea)一致性分别为94﹪、99.9﹪。

MMC possesses several types of enzyme activity, such as N-glycosidase, RNase and deoxyribonuclease activities. All its biological activities may be related to the ribosome-inhibiting activity of the protein.

MMC属于Ⅰ型核糖体失活蛋白,具有N-糖苷酶、RNase及DNase活性,α-MMC的多种生物功能可能都与其核糖体失活的性质有关。

There are some little cells with diameter 4.5~8.3 μm in molecular layer. Purkinje cell is like a pear in Purkinje cell layer, it's base width is 17.8~25.6 μm, it's height is 18-30 μm. There are lots of rough endoplasmic reticulum, free ribosome, mitochondria, golgi body, lysosome, etc. in Purkinje cell. Granular cell ratio of nucleus to cytoplasm is high in granular layer.

分子层含少量小细胞,细胞大小在4.5~8.3μm; Purkinje细胞层细胞体呈梨状,底宽在17.8~25.6μm之间,高约为18~30μm,细胞中含有丰富的粗面内质网、游离核糖体、线粒体、高尔基体、溶酶体等;颗粒层的颗粒细胞细胞核大,细胞质少。

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