核糖体

Arundinacea in vitro. After cultivation on PDA at 26℃ under continuous fluorescent light for 6 days, the colonies were black green and the hyphae were septate. Spore size was 30.6~17.0 μm×10.9~13.6 μm, mostly with four-septa. The central cell was dark and the third cell from the base was the largest. The DNA sequence of ribosomal ITS of the isolates was found to match 100% with an ITS sequence of Curvularia affinis in GenBank. This is the first report of leaf spot caused by C. affinis on F.

结果表明：该病原菌能侵染高羊茅，在26℃和光照下，在PDA培养基上培养6d后菌落呈墨绿色，菌丝有隔，分生孢子有4个横隔膜，平均大小为30.6~17.0μm×10.9~13.6μm，中部3胞暗色，第3个细胞基部膨大；其核糖体DNA-ITS序列分析表明，该菌与GenBank中近缘弯孢的同源性是100%；结合形态学特征和致病性测定，认为该菌为C。

The application of Bacillus and their nonribosomal peptides to sericulture would make it more ecological.

研究芽孢杆菌及其产生的非核糖体肽在蚕业上的应用，有利于绿色、生态蚕业的发展。

This database on the structure of ssu ribosomal subunit RNA made available to the scientific community.

这是一个为科研机构提供lsu核糖体亚单位RNA结构的数据库。

The internal transcribed spacer regions of nuclear ribosomal DNA from 8 species of subgenus Amygdalus were sequenced, and analyzed together with other ITS data of 18 species representing subgenus Cerasus, subgenus Armeniaca and subgenus Primus by using of Padus racemosa Gilib. and Padus buergeriana Yüet Ku as outgroup for studying phylogeny of subgenera in stone fruit plants.

采用核核糖体DNA内转录间隔区对桃亚属的光核桃、甘肃桃、新疆桃、山桃、陕甘山桃、扁桃、野扁桃基因序列测序及来源于GenBank的李、杏、梅、樱的18个种的ITS区基因序列，以稠李和楼木、梅和杏分别作为对桃亚属及其近缘亚属植物、桃亚属各种类的外类群，进行系统发育树的建立。

The secondary structure of 5.8S rRNA and ITS2 of Usnea was investigated and a secondary structure model for this group of lichens was proposed.

本研究以松萝属为研究对象，构建了该属核糖体5.8S和第二转录区间（ITS2）的二级结构模型，并对种间的结构差异进行了比较。

Expression of human ribosomal protein S20, and study its interaction with ZAK

题名： 表现人类核糖体蛋白S20，研究S20与ZAK之间的相关性

After thecomplete genome extraction of the strain was performed, the genomic DNA was partiallydigested by restriction enzyme Sau3AⅠ, the DNA fragments from 1 to 5Kb was clonedinto prokaryote expression vector pET-28a-c, and transformed host bacteria. The resultsshowed that we succeeded in constructing the gene expression library of haemophilusparasuis serovar 5, which is fundamental for the study of advanced gene screening. Inaddition, primer design was performed based on haemophilus influenzae in this study. In addition, PCR was performed by using genomic DNA of haemophilus parasuisserovar 5 as the template. The results demonstrated that we obtained two neo-gene:23SrRNA gene(conserved gene belonging to the large-subunit of ribosome) and adenylatecyclase gene(encodes adenylate cyclase and participates in converting adenyl nucleosidetriphosphate to cyclic adenosine3",5"-monophosphate). Furthermore, the phylogeneticanalyses between the species was performed, and neighbor-joining tree was constructedbased on comparison of 23S rRNA gene sequences, so it was illuminated betweenHaemophilus parasuis and other species in molecular evolution relationship.

选择我国流行优势菌株副猪嗜血杆菌血清5型地方株为研究对象，提取细菌基因组DNA，用限制性内切酶Sau3AⅠ对基因组DNA进行部分酶切，回收大小为1～5Kb的DNA片段，将其连接入原核表达载体pET-28a-c，最后转化宿主菌，结果成功地构建了基因表达文库，为后续的基因筛选工作奠定基础；另外，本研究选择嗜血杆菌属的流感嗜血杆菌为参考对象进行引物的设计，以副猪嗜血杆菌血清5型地方菌株的基因组DNA为模板，进行PCR扩增反应，结果表明成功地获得两个新基因：23S rRNA基因(存在于核糖体大亚基中的保守性基因)和腺苷酸环化酶基因(负责将腺嘌呤核苷三磷酸转变为环腺苷酸)，并进一步做了不同物种之间的分子系统发育分析，构建了基于23S rRNA基因的邻接法系统发育树，阐明了副猪嗜血杆菌与其它菌种的分子进化关系。

In order to clarify the taxonomic status of Dirofilaria sp. of Ailurus fulgens,the second internal transcribed spacer (ITS-2) of rDNA of Dirofilaria immitis from dogs in Ya'an in Sichuan Pro-vince and of Dirofilaria sp.from Ailurus fulgens in Chengdu and Chongqing were sequenced and compared.

为探讨采自小熊猫的恶丝虫虫种的分类地位，测定了四川雅安地区犬源犬恶丝虫和成都及重庆两地小熊猫源恶丝虫的核糖体第二内转录间隔区（ITS-2）基因序列并进行了比对分析。

This paper reviewed the application of nuclear ribosomal DNA internal transcribed spacer region sequences in phylogenetic study of angiosperms at intra\|inter genera or family levels in recent years at home and abroad. Combined with the research of Chinese \%Alpinia \%Roxb.

本文就近年来国内外有关被子植物核糖体DNA中的内转录间隔区序列在植物属内、近缘属间乃至科内系统发育研究中的应用，结合作者在中国姜科山姜属 AlpiniaRoxb 。

The results shown that:(1) Bufo raddei Strauch tadpole was capable of lens regeneration, which originated from the epithelial cells at dorsal iris margin by depigmentation.(2) Depigmentation of Bufo raddei Strauch during lens regeneration was by two methods: First, pigment granules initially dispersed all over the cytoplasm were moved towards the periphery of the cell, and then were directly taken up by the amoeboid cells. Second, pigment granules in a cell were first crowded in a mass, surrounded by a membranous structure in the cytoplasm, which were eventually discharged as a mass from the cell.

结果表明：花背蟾蜍蝌蚪(1)具有晶状体再生的能力，其晶状体的再生来源于虹膜背缘的上皮细胞；(2)虹膜背缘去色素是转分化的前提，主要有两种方式：一种也是最主要的方式是在晶状体被摘除后虹膜外侧产生巨噬细胞，巨噬细胞吞噬虹膜色素上皮细胞中的色素颗粒；另一种方式是虹膜色素上皮细胞自身释放色素颗粒；(3)电镜观察发现虹膜色素上皮细胞转分化过程中线粒体有明显变化，核糖体增多，粗面内质网增多，晶状体纤维分化过程中细胞核逐渐凝聚。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。