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Methods: Genes of MCP and DAF were connected by 10aa coding sequence of enriching Gly and Ser. Then insert into pcDAN3.1 vector, constituting pcDNA-MD3 expression plasmid of hMCP-DAF fusion gene. The genes were transformed by liposome into the pig endothelial cell; human serum handles masculine cloning, identify the function that restrain breaking of person alexin.

将MCP、DAF两个基因以富含Gly和Ser的10aa编码序列作铰链串接,并接入表达载体pcDNA 3.1中,构成含hMCP-DAF的真核表达质粒(pcDNA-MD3);以脂质体转染猪内皮细胞,人血清处理阳性克隆细胞,鉴定抑制人补体溶破的功能;应用显微注射将线性基因导入猪受精卵;PCR检测导入基因整合率。

By using the OP-10/JFC/ amyl alcohol non-ionic compound emulsifier as stabilizer, about 12% organosilicon was appended after the pre-polymerzation of MA, n-BMA, and MAA acrylate monomer was performed and the temperature was decreased to 62℃, and then the pH value was controlled in 7~8 after the polymerization.

结果表明,以OP-10聚醚渗透剂JFC/戊醇非离子复合乳化剂为稳定剂,在MA、n-BMA、MAA丙烯酸酯单体形成预聚体后降温至62℃,加入12%左右的有机硅单体,反应结束后控制pH值7~8,微乳液具有良好的稳定性,乳胶粒具有翻转型的核-壳结构。

Algae, echinoderm, bivalve, and reef-building organisms including bryozoan, coral, stromatoporoids, and sponge are conserved in the carbonate of the Yijianfang Formation.

通过对塔河南盐下地区28口钻井岩心中奥陶统一间房组的详细描述和3 200余块薄片的鉴定,确认其主要的岩石类型有生屑灰岩、生物灰岩、藻球粒灰岩、含核形石灰岩、鲕粒灰岩、内碎屑灰岩、微晶灰岩、海绿石灰岩。

By 42 days postvaccination, pigs in group 1 and group 2 had seroconverted to PCV2 capsid antibody.

在免疫后42天,PCV1-2组及真核表达质粒组产生了PCV2抗体。

In vivo Location demonstrated that Septin 1 is located in centriole before mitosis, and later slowly moved to the milieu of nucleus.

细胞内定位显示:Septinl在有丝分裂前定位于中心粒,后慢慢迁移至核周围。

Tumor associated protein n35 was located on the centriole of the chromosomal domain at the s-g2 phage.conclusions the tumor associated protein n35 might be a tumor cell growth regulator, expressed only it's the cancer cells and related with the proliferation of cancer cells.

结果 肿瘤相关蛋白n35不存在于正常人淋巴细胞、正常肺细胞及人皮肤纤维细胞中,而存在于肺癌细胞蛋白中,在亚细胞结构中主要分布于胞核,在肿瘤细胞有丝分裂进入g2至m期时明确地定位于中心粒结构上。

It distributed in the live lung cancer cell GLC-82, but not in the protein ingredient of the normal human lymphocyte, the normal human lung cell and human skin fibroblasts, Tumor associated protein N3S was located on the centriole of the chromosomal domain at the S-G2 phage.

结果:肿瘤相关蛋白N35不存在于正常肺细胞、人淋巴细胞及人皮肤纤维细胞中,而存在于肺癌细胞蛋白中,在亚细胞结构中主要分布于胞核,在肿瘤细胞有丝分裂进入S至G_2期时明确地定位于中心粒结构上。

Telomeres, short tandem repeat sequences at the ends of eukaryotic linear chromo somes, function to stabilize chromosomes.

端粒是真核细胞染色体末端的重复序列,对维持染色体的稳定性具有重要作用。

In this dissertation, based on glass microspheres and submicron silicon dioxide, the silver-coated core-shell shielding particles were prepared by liquid phase chemical deposition and layer by layer packing technology. The influences of the preparation conditions on the structure of the synthesized core-shell particles were also systemically studied. The conclusions are enumerated as follow:(1) Monodispersed submicron silicon dioxide spheres in the size range of 250~550 nm are synthesized by Sol-Gel method using ammonia as catalyst.

本文在前人研究的基础上设计出以微米级玻璃微珠为基核,采用液相化学沉积法和层层包裹法制备表面包银的核壳粒子电磁屏蔽剂,并对液相化学沉积法和层层包裹法的制备工艺进行了系统研究,取得了以下主要结论:(1)以氨水作催化剂,采用溶胶-凝胶法制备了粒径分布在250~550 nm之间的二氧化硅微球。

Single component and multi\|component of iron system colloidal catalyst \Ali\|Bu\-3CH\-2CHCH\-2Cl\ were mixed by different ratios in hydrogenated gasoline medium at 25℃.This paper studied the relationship between the nonaqueous systematic electroconductivity and the concentration of catalyst components.In combination with Tyndall effect,polymerization experimental results,Ali\|Bu\-3 existed in associated state and dissociated into ionpair.The reaction between Ali\|Bu\-3 and Fenaph\-2 was the main reaction which formed colloidal core.Ali\|Bu\-3 reacted on CH\-2CHCH\-2Cl and Ali\|Bu\-2Cl was yielded which joined the formation of active center on the surface of colloidal core.The excessive Ali\|Bu\-3 was dissociated into ionpair which formed double electrode layer,and the double electrode layer kept the colloidal particles relatively stable.

研究了在25℃加氢汽油介质中,铁系胶体催化剂[Fenaph2Ali-Bu3―CH2==CHCH2Cl]单组分、多组分按不同配比混合,非水体系电导率与浓度的关系,结合Tyndall效应,聚合实验结果,得出Ali-Bu3以缔合状态存在并解离成离子对它同环烷酸亚铁的作用是形成胶核的主要反应,与氯丙烯反应生成的氯化异丁基铝,在胶核表面参与形成活性中心由过量Ali-Bu3解离的离子对形成的双电层结构,使胶粒保持相对稳定。

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