核粒

The omp17.3 gene of Brucella abortus was amplified by PCR and then the amplicon was cloned into the eukaryotic expression plasmid pcDNA3.1 to construct a recombinant plasmid pcDNA3.1-omp17.3. Then the recombinant plasmid pcDNA3.1-omp17.3 was transfected into COS7 cells, and the expressed OMP 17.3 was detected by Western-blotting.

采用PCR方法扩增了布鲁氏菌17.3ku外膜蛋白编码基因，并将该基因克隆至真核表达载体pcDNA3.1中，成功构建了真核表达质粒pcDNA3.1-omp17.3.pcDNA3.1-omp17.3转染COS-7细胞后，通过Western-blotting检测到了17.3ku蛋白的瞬时表达。

This means that the ACA antiserum can actually and specifically recognize the protein components of centromere/kinetochore.

这看来是着丝粒蛋白在单细胞真核生物间期核中的分布上的特点。小眼虫细胞质中有一些阳性反应的颗粒，可能为线粒体或叶绿体的成份。

Hypotrichids ciliate macronucleus chromosome was named "genesize chromosome", which carrying the gene coding sequence flankedby short non-translated regions and bounded by telomeres (C_4)_4(A_4)_3.Macronucleus chromosome is furthest simple chromosome ineukaryotic cell.

下毛类纤毛虫的大核染色体被称为"基因大小染色体(gene-size chromosome)"，其含有一个基因编码序列，5′和3′端各有一段基因表达调控序列以及端粒序列(C_4)_4(A_4)_3，是真核生物中结构最简约染色体。

Primary culture and subculture were adopted using lung cells of a female Elaphodus cephalophus' embryo. A new karyotype and a pair of big telocentric chromosomes were found. The diploid has 48 chromosomes, and the karyotype formula is 2M+2ST+42T+XX. C-banding analysis shows that these chromosomes are homologous with those reported relevant chromosomes. This article is the first to report the polymorphism of Elophodus cephalophus B-chromosomes and studies it's transmitted mechanism.

采用原代培养和传代培养法，对1头毛冠鹿的胚肺组织细胞进行观察，发现一种新的核型，其二倍体染色体数目为2n=48，核型公式为2M+2ST+42T+XX，出现1对大的末端着丝粒染色体，C-带显示该染色体与已报道的相关染色体同源，首次提出毛冠鹿B染色体多态，对其传递机制进行了探讨。

The accumulation of starch, vacuolation of multinucleate microspores and degeneration of anther wall affected microsporic embryogenesis.

淀粉粒的积累，多核小孢子的液泡化以及花药壁的衰退都可影响雄核发育。

The tectonic rocks along the fault zone are composed of cataclastic rocks and mylonites formed in higher, middle and lower temperature. Respectively, both of structural features and rocks have the features of multiple phase's of deformation. The deformation features of main minerals in the high temperature mylonites show that: the quartz has the form of long single crystal and the fabric feature of point maximum near X axis; the feldspar has the form of recrystallization assemblage, They present a high amphibolite-granulite facies with temperature ＞600℃ and depth ＞20km. The deformation features of mylonites formed in middle temperature show that: the quartz has the form of polycrystal banding and the fabric feature of point maximum near Y axis and small circle girdle with Y rotational axis; the feldspar has the form of a coremantle structure, They reflect a high greenschist-lower amphibolite facies with temperature from 400℃ to 600℃ and depth from 15km to 20km.

其中，高温糜棱岩主要矿物变形特征组合为：石英为长矩状或长条状单晶、组构为近X轴的点极密，长石为几乎完全重结晶的集合体，形成温度＞600℃，相当于高角闪岩—麻粒岩相，形成深度＞20km；中温糜棱岩主要矿物变形特征组合为：石英为多晶条带、组构为近Y轴点极密和以Y轴为旋转轴的小圆环带，长石发育核幔结构，形成温度为400～600℃，相当于高绿片岩—低角闪岩相，形成深度为15～20km；低温糜棱岩主要矿物变形特征组合为：石英发育核幔结构、组构特征为近Z轴点极密，长石以碎裂为主，形成温度＜400℃，相当于中低绿片岩相，形成深度为8～12km；碎裂岩则主要代表了地表浅层的变形。

Aim To construct eukaryotic expression vector containing chimeric gene G1S0.7 of Hantavirus and express it in eukaryocyte.

目的 构建含汉坦病毒嵌合基因G1S0.7的真核重组质粒，并在真核细胞中有效表达。

1,After transfected the mutated mtDNA of colorectal carcinoma,the mtDNA D-loop region of the transfected cells displays new mutation points.2,The external source pieces of the mutated mtDNA can integrate to nuclear genome after transfection.3,There's no differences in apoptosis between combinations after transfected the mutation of mtDNA in NIH3T3 and LST cells.4,The mutated mtDNA may affect the action mechanism of occurrence and development in colorectal carcinoma through affecting its mtDNA mutation or integrating exogenetic mtDNA to its nuclear which may cause the abnormal expression of oncogene or anti-oncogene.

（1）转染突变的大肠癌细胞mtDNA后转染细胞的mtDNA均可发生多处的突变位点。（2）通过转染后突变的外源性的mtDNA可以整合到核基因组内。（3）突变的mtDNA转染LST细胞及NIH3T3细胞后，不影响转染细胞的凋亡改变。（4）mtDNA的突变可能通过影响体细胞mtDNA的突变和通过外源性mtDNA在核内的整合从而影响癌基因或抑癌基因的表达异常，从而参与肿瘤的发生发展。线粒体DNA；D―环区；突变；质粒；pcDNA3.1；转染

The silica/Polystyrene monocore-shell nanocomposite particles(95nm in diameter) were synthesized through miniemulsion polymerization by using sodium dodecyl sulfate surfactant,hexadecane costabilizer in the presence of silica particles(50nm in diameter) coated with methacryloxytrimethoxysilane.

采用细乳液聚合法，以3-甲基丙烯酰氧基丙基三甲氧基硅烷(KH570)表面改性的直径50nm的氧化硅粒子为核，在乳化剂、助乳化剂、引发剂存在的情况下制备了小粒径、单核核壳结构氧化硅/聚苯乙烯纳米复合微球。

To design HDV ribozyme for human telomerase RNA component, and observe its inhibition for telomerase activity .

设计针对端粒酶的HDV核酶，观察其对端粒酶活性抑制。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。