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With an orthogonal experiment design , the telomerase inhibitors [azidothymidine , AZT 300 mg/ and lamivudine 150 mg/, per os , bid ,×2 weeks ] and X2irradiation [ total dose 10 Gy (2 Gy ×5) in 1 week ] were used to treat BALB/ c mice carrying breast cancer MA782 for evaluating the influence on peripheral blood cells , bone marrow nucleated cells and telomerase activity.

为了探讨端粒酶抑制剂联合X 线照射对荷瘤小鼠的骨髓抑制作用,采用正交实验设计,应用端粒酶抑制剂[AZT 300 mg/, lamivudine 150 mg/ ]联合X 线照射(2 Gy ×5次/ 1 周)处理移植性乳腺癌(MA782 )小鼠,观察其对骨髓有核细胞、外周血像及骨髓端粒酶活性的影响。

Among these features are: absence of the nuclear rotation and development of the centriolar complex and the initial region of the axoneme in a position directly perpendicular to the basal pole of the nucleus and presence of numerous thick anchoring fibrils connecting the proximal centriole and the proximal part of the distal centriole to the nucleus.

其特点主要是:生精过程中不发生细胞核的旋转,中心粒复合体和轴丝起始段直接发生在核的基底面垂直线上,有无数的粗的固定纤维将近端中心粒和远端中心粒的近侧部连接到细胞核上。

More levels of nano-SiO〓 lead to larger particle size. An increase in polymerization reaction temperature causes the decrease in coagulum and increase in viscosity and particle size, and the nano-SiO〓 has same effects at certain temperature. When the levels of hydrophilic monomer acrylic acid and hydroxylethyl methacrylate in core composite are increased, the particle size of emulsifier-free emulsion decreases, in spite of the presence of nano-SiO〓.

无皂乳液具有单分散性好、表面洁净、成本低廉、不影响产品性能的特点,首次研究了含或不含纳米粒子的含羟基和羧基的丙烯酸无皂乳液聚合,发现含或不含纳米粒子的无皂乳液聚合后的粒子粒径较大,具有单分散性;较多的纳米粒子导致较大的乳液粒径;反应温度升高,凝聚物含量下降,而乳液粘度和乳胶粒粒径增加,在相同的反应温度下,纳米粒子降低了聚合过程中的凝聚物含量,增加了乳液聚合物的粘度和粒径;不论纳米粒子是否存在,当无皂乳液核组成中亲水单体AA和HEMA用量增加时,乳胶粒粒径均减少。

Poly/melamine cyanurate composite latex particles are prepared via soapless emulsion polymerization. XPS data and TGA analysis reveal that MC particles are encapsulated by PMMA during polymerization, resulting in the formation of a core-shell structure of PMMA/MC composite latex particles. MC can affect the thermal degradation and promote the charring of PMMA. The application of PMMA/MC in nylon 6 (PA6) indicate that the composite latex particles can improve the mechanical pro...

采用原位乳液聚合法制备了聚甲基丙烯酸甲酯/三聚氰胺氰尿酸盐复合胶粒,对其进行X光电子能谱及热重分析结果显示,在MC阻燃剂颗粒表面覆盖了一层PMMA胶膜,形成了具有核-壳结构的PMMA/MC复合胶粒;MC能影响PMMA的热降解过程并促进其成炭;在尼龙6(PA6)中的应用结果表明,PMMA/MC复合胶粒能改善PA6的力学性能和阻燃性能。

When Tetrahymena, Euglena, Crypthecodinium and Oxyrrhis were stained with the same technique, although all the nuclei gave positive reactions, no precentromeres could be distinguished; within nuclei there were only patches composed of minute fluorescent spots seen.

在对小眼虫和寇氏隐甲藻作制备核骨架的细胞分级抽提处理后,细胞核仍还都呈阳性反应。这表明与高等真核生物的相似,低等真核生物的着丝粒蛋白也是与核骨架紧密结合的。

However, there are still several safety concerns about the use of a DNA vaccine, which include the possibility of integration into the host genome, adverse immunopathology, and anti-DNA autoantibody induction.Attempting to solve some of these problems, eukaryotic expression plasmids pTL-8/ AMA-1 and pTL-8/ AMA-1 which express trans-activator or reverse trans-activator, respectively, and AMA-1 gene of Plasmodium falciparum were constructed by using tetracycline regulable system.

首先构建了恶性疟原虫顶第四军医大学硕士学位论文端膜抗原1(AMA一1)基因和反式活化因子基因的真核表达质粒pTL一8/灿认一l和PTL一8/灿认一1,并大量制备这两种质粒及表达转录抑制子的质粒pUHS6一1。

Effect of Different Sowing Date and Densities to the Yield of Avena nuda L .Variety Huazao No.2;2. The paper analyses the chromosome karyotype of Avena nuda L .

对莜麦染色体的核型进行了分析,结果表明其染色体数为42,为六倍体,包括11对中部着丝粒染色体,9对近中部着丝粒染色体和1对近端着丝粒染色体,其中有3对染色体带有随体。

A small fraction of bacterial chromosomes (such as those in Streptomyces and Borrelia) is linear and possess telomeres, which are very different from those of the eukaryotic chromosomes in structure and functions.

一小部分细菌的染色体(如链霉菌属和疏螺旋体属细菌的染色体)是线性的并且具有端粒,但是它们的端粒与其他的真核生物的端粒在结构和功能上有很大的区别。

Objective Establishment of a hybridoma cell line secreting a monoclonal antibody to facilitate iˉdentification of human tissue kallikrein(KLK1gene,hK1protein).Methods Mice were immunized with E.coli-exˉpressed GST-hK1fusion protein and their spleen cells were fused with SP2/0myeloblastoma cells.Specificity of the monoclonal antibody was shown by Western blotting and by immunofluorescence.Results The monoclonal antibody reacted specifically to E.coli-expressed hK1and with the KLK1cDNA-transfected COS-1cells.

目的 将人组织激肽释放酶(基因命名 KLK1,酶命名为hK1)cDNA克隆入肠杆菌表达质粒,以重组菌表达的GST-hK1融合蛋白免疫小鼠,获得了分泌hK1特异单克隆抗体的杂交瘤细胞株方法将KLK1cDNA克隆入真核表达质粒,用获得的重组质粒转染COS-1细胞,经间接免疫荧光试验证明,上述单克隆抗体能与转染细胞发生特异反应。

Telomerase enzyme, containing a catalytic subunit, the human telomerase reverse transcriptase, and a small integral RNA component, synthesises the telomeres, the ends of eukaryotic chromosomes.

端粒酶包含有一个催化亚基——人端粒酶逆转录酶,与一小段RNA组分共同合成端粒。端粒位于真核细胞染色体的末端。

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This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

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双标记神经干细胞的增殖、分化活力与未标记神经干细胞相比无改变。