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Coupled with enrichments of Pb and Sr at the rim of the interstitial rutiles, Zr contents decrease from the core to the rim.

粒间金红石中,同一颗粒金红石核部Zr含量系统高于边部,而边部则出现了明显的Pb和Sr富集特征。

METHODS: Fulllength or fractions of VP22 were fused to C terminal of HBV core protein, and cloned into pcDNA3.1 vector, yielding eukaryotic expression plasmids of DN mutant.

将VP22全长及其不同区段融合于HBV核心蛋白的C端,克隆入pcDNA3.1构成DN突变体真核表达质粒。

Objective To construct the eukaryotic expression vector for pathogenic gene MYOC of primary open angle glaucoma and express in COS-7 cells.

目的 构建原发性开角型青光眼致病基因MYOC的真核表达质粒,并在COS-7细胞中表达MYOC蛋白。

Methods pVAX1-GRA8 was purified and transfected into Africa green monkey kindey vero cell lines,expression of GRA8 in vero cell lines were detected by RT-PCR and immunoblot with rabbits sera infected with Toxoplasma gondii.

大量提取纯化重组真核表达质粒pVAX1-GRA8,瞬时转染培养的vero E-6细胞,RT-PCR和免疫印迹法检测GRA8在细胞中的表达。

AIM: To construct the eukaryotic expressing vector of human cbl and test its expression in African green monkey kidney cell line COS 7. METHODS: Human cbl gene tagged with flag was amplified from pEFHAcbl plasmid by PCR. The product was cloned into pGEM T easy, sequenced and then subcloned into eukaryotic expressing vector pcDNA3.1.

以含有人全长cbl cDNA的质粒pEFHAcbl 为模板,采用PCR方法扩增cbl基因,并在其N 末端带上含24 bp的flag标签,克隆到pGEM T easy载体并测序,再亚克隆至真核表达载体pcDNA3 1,酶切鉴定正确后采用脂质体法瞬时转染COS 7细胞,Western blot检测flag cbl在细胞中的表达。

METHODS: Human cbl gene tagged with flag was amplified from pEFHAcbl plasmid by PCR. The product was cAIM: To construct the eukaryotic expressing vector of human cbl and test its expression in African green monkey kidney cell line COS7. METHODS: Human cbl gene tagged with flag was amplified from pEFHAcbl plasmid by PCR. The product was cloned into pGEMT easy, sequenced and then subcloned into eukaryotic expressing vector pcDNA3.1.

以含有人全长cbl cDNA的质粒pEFHAcbl 为模板,采用PCR方法扩增cbl基因,并在其N末端带上含24 bp的flag标签,克隆到pGEMT easy载体并测序,再亚克隆至真核表达载体pcDNA31,酶切鉴定正确后采用脂质体法瞬时转染COS7细胞,Western blot检测flagcbl在细胞中的表达。

Methods: Oocytes in the GV stage were separated from ovary by squeezing method. In mouse germinal vesicle GV stage, the expression of ATP8 gene in the mitochondria in the single oocyte was detected by RT-PCR, in which, cDNA was synthesized with two methods: one was the single GV-stage oocyte directly to be placed RT, the other was to perform RT after eliminating mtDNA and nucleus DNA with the EeoR Ⅰ enzyme and Dnase. And the product of RT-PCR was cloned and sequenced.

应用挤压法从卵巢中分离获得生发泡期(germinal vesicle, GV)卵母细胞;用RT-PCR检测GV期单个卵母细胞中ATP8基因的表达:其中cDNA的合成分两种方法进行:一是将GV期单个卵母细胞直接进行RT合成cDNA,二是先用DNA酶加EcoR Ⅰ酶祛除mtDNA和核DNA后再进行RT;回收产物构建克隆质粒并测序。

A eukaryotic expression plasmid of Fas/APO-1 gene was constructed successfully.

本实验成功地构建了Fas/APO-1基因的真核表达质粒。

The BALB/c mice were immunized i.m. with the plasmids for 3 times in 3 weeks intervals.

用重组真核表达质粒pcDNA3 1 /HRP Ⅱ经肌肉免疫小鼠 3次,每次间隔 3wk。

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This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。

There is no differences of cell proliferation vitality between labeled and unlabeled NSCs.

双标记神经干细胞的增殖、分化活力与未标记神经干细胞相比无改变。