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Results The eukaryon expression system pcDNA3.1/myc-HisC-bFGF could express the target protein bFGF in vitro. The recombinant plasmids, pcDNA3.1/myc-HisC-bFGF and pCD2-VEGF121 were transferred into muscles flap in vivo successfully. The active proteins bFGF and VEGF121were expressed at high levels.

结果 构建的pcDNA3.1/myc-HisC-bFGF真核表达体系成功转染体外培养HeLa细胞,目的基因在mRNA水平和蛋白水平均有表达。pcDNA3.1/myc-HisC-bFGF和pCD2-VEGF121重组质粒分别转染在体肌瓣,获得外源基因高水平表达。

The full hBD2 cDNA was inserted into the multi clone site of a eukaryotic expressive plasmid pcDNA3.1/Myc His and located closely at the upstream of two tag gene (myc and 6 Poly histidines ) so as to construct another recombinant eukaryotic expressive vector of hBD 2 gene: rpcDNA3.1/Myc Hi...

将 h BD- 2的全长 c DNA片段插入编码双重报告基因 Myc和 6个多聚组氨酸的真核表达质粒 pc DNA3.1/Myc- His ,构建出 C端带 Myc和 6× His的 rpc DNA3.1/Myc-His /h BD- 2。

We consider that the better system of measuring for the size of granular mixtures may be the number of elements. So we designed another kind of large system experiment, the experiment of Tokamak plasma system with the characteristic of jillion element. Under the condition of Ohmic heating and Low confinement mode, the interaction among elements will increase with the debasement of plasma confinement, causing SOC.It validates the assumption that SOC mostly depends on the mechanism of interaction among elements in a large system, in other way.

为通过精密可控实验手段探讨大尺度散粒体组元之间相互作用强度对SOC的影响,利用磁约束受控核聚变托卡马克装置实验数据,在欧姆加热或L模放电的条件下,对HL-1M边缘区等离子体粒子输运的统计性质分析发现,反常输运具有SOC的特征。

The particle size and flow behavior of the latexes,dynamic mechanical properties,tensile behavior,and water resistance of the copolymers,as well as the nucleation mechanism of latex particles were investigated.

对乳胶粒粒径大小、乳液流体力学行为、共聚物的动态力学性质、拉伸行为及耐水性进行了研究,并对乳胶粒的成核机理进行了探讨。

The contact angle of complex coatings composed by latexes with different sizes of particles, glass transition temperatureT(subscript g and core-shell structure were measured and the effect of binary collaboration was also discussed.

将粒径不等、T不同的乳液复配、核壳结构不同的乳液复配,并测定了复配物成膜后的接触角,讨论了它们可能产生的二元协同作用。

Objective To construct recombinant plasmid of Legionella pneumophila mip gene and detect its expression in NIH3T3 cells.

目的 构建嗜肺军团菌mip基因的真核表达重组质粒pcDNA3.1-mip,并研究其在细胞中的表达。

This study has built a foundation for the development of PAL gene DNA vaccine for Legionella pneumophila.

成功构建了真核表达重组质粒PcDNA3.1-PAL为嗜肺军团菌核酸疫苗的进一步研究奠定了基础。

Objective: To study the anti-tumor effect of PTEN eukaryotic expressing plasmid on human bladder cancer xenografts in nude mice.

目的:探讨PTEN真核表达质粒对人膀胱癌裸鼠移植瘤的抑制作用。

OBJECTIVE: To transfect rabbit bone marrow mesenchymal stem cells using pIRES2-EGFP-BMP-2 eukaryotic expression plasmid.

目的:使用前期已经构建成功的pIRES2-EGFP-BMP-2真核表达质粒转染兔骨髓间充质干细胞。

Loss of telomere DNA repeats in eukaryotic cells is associated with senescence and apoptosis.

真核细胞端粒DNA序列的丢失与细胞的衰老及凋亡有关。

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This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。

There is no differences of cell proliferation vitality between labeled and unlabeled NSCs.

双标记神经干细胞的增殖、分化活力与未标记神经干细胞相比无改变。