核粒

She spat out a cherry stone.

她吐出一粒樱桃核。

Methods Fourteen mutated vpr fragments were selected from patients with HIV. Both eukaryotic expression vector pcDNA3.1 and PCR products were purified, double-cut by Hind Ⅲ and BamH Ⅰ, and the cut products were legated and transformed into competent cells JM109. The 14 reconstructed plasmids were transfected into Hela cells. Cells with pcDNA vpr-wt, pcDNA vpr-Fs and pcDNA3.1 blank cells, and without pcDNA3.1 cell were established.

以14个带有HIV-1vpr基因片段的PcD-NA3.1真核表达载体构建重组质粒，将其转染Hela细胞，并设立保守株vpr基因转染细胞、突变株vpr-FS基因转染细胞、空载体转染细胞和未转染细胞作为对照，经逆转录多聚酶链式反应检测目的基因转染成功后，Pi染色，用流式细胞仪检测被转染细胞的细胞周期分布和细胞凋亡率。

Methods 14 mutanted vpr fragments selected from Chines patients with HIV. Both eukaryotic expression vector pcDNA3.1 and PCR products purified , double-cut by HindⅢ and BamH and the cut products legated and were into competent cells JM109. The 14 reconstructed plasmids electronically transfected into hela cells, and established cells with pcDNA vprwt 、pcDNA vpr-Fs and pcDNA3.1 blank cells, and without pcDNA3.1 cell.

将14个带有HIV-1 vpr基因片段的pcDNA3.1真核表达载体构建重组质粒，将其转染Hela细胞，并设立保守株vp r基因转染细胞、突变株 vpr-FS基因转染细胞、空载体转染细胞和未转染细胞作为对照，经RT-PCR检测目的基因转染成功后，经Pi染色用流式细胞仪检测被转染细胞的细胞周期分布和细胞凋亡率。

When applied the spiny eel X chromosome library probes to the chromosomes of the two fish, different signals were distributed: in Siniperca chuatsi, besides some little signals on lot of autosomes, a large bright signal region was detected on the 8th chromosomes, which represented a synteny of spiny eel X chromosomes; but in Channa argus, no distinct synteny was found.

当以刺鳅的生物素标记X文库探针对鳜鱼和乌鳢的有丝分裂染色体进行染色体涂绘后，经过核型分析和统计分析表明，在这两种鱼的染色体上的杂交信号分布是不同的：在乌鳢中，除散在信号外，在其第八号染色体近着丝粒部位存在着一段较大的信号结合区域，应是与刺鳅的X染色体同源的保守同线群，而在鳜鱼中，只有散在信号分布于多条染色体上，没有明显的保守同线群存在。

Objective To construct the eucaryotic recombinant plasmid of pYES2/LactoferricinB and express in yeast of S.cerevisiae and preliminary verify the antibacterial activity.

目的构建牛乳铁多肽的真核表达质粒pYES2／Lactoferricin B，实现其在酿酒酵母S.cerevisiae中的表达，初步检测其不同变异的体外抗菌活性。

RESULTS: All the four recombinant eucaryotic plasmids were constructed successfully, and HBV core protein expression was confirmed by internal reference for transfection.

结果： 4份重组真核表达质粒均构建成功，转染至HepG2后通过内参照EGFP可鉴定出均有HBV-C蛋白表达。

Objective To construct the eucaryotic recombinant plasmid of pYES2/LactoferricinB expressing in yeast of S. cerevisiae, of which the expressed protein antibacterial activity was verified in preliminary.

目的 构建牛乳铁多肽的真核表达质粒pYES2/Lactoferricin B，实现其在酿酒酵母S.cerevisiae中的表达，并初步检测其不同变异的体外抗菌活性。

The eukaryon expression vector of bFGF is constructed and can be expressed successfully in vitro and in vivo. That is a primary preparation for the research on tissue transplantation and tissue engineering with bFGF gene therapy.

构建了人bFGF真核表达质粒，并可在体内外顺利表达，为进一步组织移植或组织工程基因治疗研究奠定了基础。

OBJECTIVE:To study the effects of eukaryon expressed adiponectin on the glycogen synthesis and glucose oxidation in skeletal muscle cell strain C2C12 myotubes by transfecting plasmids carrying mouse adiponectin.

目的：通过转染带小鼠脂联素基因的质粒，观察真核表达的脂联素对骨骼肌细胞株C2C12肌小管葡萄糖氧化和糖原合成的影响。

Objective To construct recombination eukaryon expression plasmid for human parathyroid hormone gene, assay PTH expression and biological activity after transfection in vitro and evaluate gene therapy effect on hypoparathyroidsm.

目的构建甲状旁腺激素基因的重组真核表达质粒，评价体外转染后PTH基因的表达与生物学活性，同时观察其对甲状旁腺功能减退动物的基因治疗作用。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。