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In order to further study, with a new method of preparation of DNA fibers which has conquered the disadvantage of the way of squashing in liquefacient nitrogen,high-quality fibers were been gotten from maize which has large genome and contains numerous types of highly repeat DNA elements.

为了进一步分析,对现有的提核方法进行了改进,采用了新的方法制备DNA纤维,克服了液氮研磨法的不足,并在基因组比较大,重复序列较多的玉米上获得成功,同时用伸展DNA纤维的荧光原位杂交技术测定了端粒DNA在玉米染色体上的拷贝数。

The article studied Eisenberg model, Forsman model, Dreyfus model, Bonnoto Bonner, Longworth, Vaughan model and Core-shell model. A BAB-triblock copolymer was employed as macromolecular model. Minimized Gibbs free energy relates the micellar structure parameters to parameters of the triblock copolymer quantitatively. Furthermore, it could be explained theoretically that the micellar diameter and the equilibrium aggregation number both decrease with ion content.

然后讨论了BAB型三嵌段模型、Eisenberg模型、Forsman模型、Dreyfus模型、Bonnoto和Bonner、Longworth和Vaughan模型与核-壳模型,并以BAB型三嵌段离聚物为模型大分子,建立了胶束特征参数与聚合物结构参数之间的定量关系,从理论上解释了胶体粒径和聚集数随离子含量的增加而降低的原因。

Interval observations under thefluorescent microscop were performed with excition waves of 470—490 nm.The results displayed that GFP genes were expressed effectively in E.octocarinatus, and GFP was distributed unifimly around macronuclearand diffused to the entire cell gradually. The chromosomes can be kept inthe macronucleus for 90h.

用优化的脂质体转化方法将含有人工染色体的重组质粒pBTub-tel2转化到处于有性生殖分裂间期阶段的游仆虫细胞中,结果表明,GFP基因在游仆虫细胞中得以高效表达,36-48小时绿色荧光均匀分布在细胞核周围,逐渐扩散到整个细胞,染色体能够在大核中保持约90小时。

Objectives To explore the early diagnosis of atypical aplastic anemiaby analyzing the characteristic patterns of the megakaryocyte,particles and biopsy of bone marrow.

目的分析不典型再生障碍性贫血患儿的骨髓巨核细胞、骨髓小粒的特点,对其诊断进行探讨。

But,some functional cells containdistinct starch grains like mature megaspore and single nucleate embryo sac which isin the station before division.

同样,从单核胚囊分裂开始到八核胚囊刚形成也未观察到淀粉粒。

To investigate the ability of ADA isozyme expression to discriminatebetweem lymphoid and nonlymphoid cell types in acute leukemia,the gelfiltration with FPLC system was used as micromethod for ADA isozymeanalysis.

为进一步阐明白血病细胞ADA同工酶生化性质,探讨制备ADA同工酶抗体的可能性,我们采用分子筛FPLC和阴离子交换FPLC系统二步法分离纯化健康人血清、单个核细胞和急性粒单核细胞白血病细胞ADA同工酶,获得部分纯化ADA1和ADA2,ADA1纯化71.62倍,活力62.2u/mg pr,ADA2纯化37倍,活力42.8u/mg pr,ADA总回收率44%,2步FPLC层析法酶活力回收率分别为68.4%和81.5%。

Using micropipette aspiration technique and immunohistochemistry, the effects of staphylococcus. aureus and its metabolite on the rheological characteristics and cytoskeleton of PMN were studied.

利用微管吸吮技术和免疫组化染色,研究了在不同浓度金黄色葡萄球菌及其代谢液作用下多形核中性粒白细胞粘弹性的变化,以及在金黄色葡萄球菌作用下PMN细胞骨架的改变。

In most multicellular eukaryotes, telomerase is only active in germ cells.

在大多数多细胞真核生物中,端粒只在生殖细胞中有活性。

Part Ⅱ Objective: To study the immunoprotective and the immunotherapic effects of the new nucleic acid vaccine of the hepatitis B: pVAX1-C3d-S2S; respectively in normal and HBV transgenic mice after being inoculated intra- muscularly with this Eukaryotic Expression Plasmid, such as the in situ production of the expressed protein in muscle tissue section and specific humoral immune response in normal mice、the variation of specific antigen、antibody and HBV DNA, especialy the changes of specific antigen in liver.

第二部分新型乙肝核酸疫苗pVAX1 C3d-S〓S免疫预防效应和治疗作用的研究目的:研究含有新型分子佐剂补体C3d的HBV adw血清型的乙肝核酸疫苗重组真核表达质粒pVAX1-C3d-S〓S接种正常BALB/c小鼠和HBV转基因小鼠后,其免疫预防作用(接种局部肌肉中特异性抗原蛋白的表达和所诱生的体液免疫应答规律)以及免疫治疗作用(血清中特异性抗原的表达、特异性抗体的产生、HBV DNA的消长和肝组织中抗原表达的变化)。

Study on mechanism of repairing myocardial necrosis by skeletal myoblast transplantation [J].

血管内皮细胞生长因子真核表达质粒的构建及其在心肌细胞中的表达[J]。

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This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。

There is no differences of cell proliferation vitality between labeled and unlabeled NSCs.

双标记神经干细胞的增殖、分化活力与未标记神经干细胞相比无改变。