核粒

PART FOUR EFFECTS OF MIL-4RA EXPRESSION VECTOR ON ASTHMATIC AIRWAY INFLAMMATION AND TH1/TH2 CELL DISFUNCTION THROUGH INTRAPERITONEAL ADMINISTRATION In the study of part three, we treated the mouse asthmatic model by intratracheal administration with mIL-4RA expression plasmid and observed obvious therapeutic effects. But intratracheal administration belongs to one of the insult therapies.

第四部分：腹腔注射mIL-4RA重组载体对哮喘小鼠气道炎症及Th失衡的干预作用前一部分研究中我们通过气道局部干预途径，即气管内直接滴注的方式，观察到mIL-4RA真核表达质粒对哮喘小鼠具有很好的治疗作用，但气管内滴注毕竟属有创性治疗，存在需要实验条件高，有较大的风险性，不易反复进行等缺点，这势必大大限制其在动物实验及进一步临床研究中的实际应用价值。

Objective:To measure the proportion of monocyte-macrophage(CD64~+)in AA patient s bone marrow,the expression of RCAS1 mRNA in bong marrow mononuclear cells,and the influence of RCAS1 to colony forming unit-erythrocyte,colony forming unit-common precursor of granulocyte and macrophage;evaluate the monocyte-macrophage(CD64+),RCAS1 and their influence to the hematogenesis;explore the role of MPS in the pathogenesis of AA.

中文摘要：目的：测定再生障碍性贫血患者骨髓中单核—巨噬(CD64~+)细胞的比例，骨髓单个核细胞RCAS1 mRNA的表达水平以及RCAS1对红系形成集落、粒-单形成集落的抑制程度，评价单核—巨噬(CD64~+)细胞及其分泌的RCAS1对造血功能的影响，探讨单核-吞噬细胞系统在AA发病机制中的作用。

Objective To amplify the hexokinase gene of Plasmodium falciparum isolate FCC1/HN, compare the sequence with that of other P. falciparum isolates and human, and construct its prokaryotic and eukaryotic expression plasmids.

目的 扩增恶性疟原虫FCC1／HN株的己糖激酶编码基因，构建其原核和真核表达重组质粒，测定其序列，比较它及它推导的蛋白质与恶性疟原虫其他株和人之间的差异。

Results of XRD,SEM,and UV-Vis indicated that ZnS nanocrystals can grow in and fill in the void of opal template,and SiO2 microspheres of templates are dissolved so as to form some three dimensional ordered porous structure and to form the inverse opal ZnS photonic crystals. Compared with opal templates,ZnS-opal and inverse opal ZnS photonic crystals assembled with SiO2 spheres in the same diameter show good optical properties and exhibited photonic band gap. The photonic band gap position of inverse opal ZnS moves towards UV area compared with opal and ZnS-opal.

结果表明：溶剂热法多次充填可使ZnS纳米晶在模板密堆积形成的空隙中均匀成核；经过酸处理的ZnS-opal中SiO2微球溶解、坍塌，形成蜂窝状三维有序介孔和反opal结构ZnS基光子晶体；相同粒径SiO2微球组装的opal模板、ZnS-opal以及反opal结构ZnS光子晶体均表现出光子带隙特性，但反opal结构ZnS光子晶体带隙位置相比前两者发生了蓝移。

Objective Consequently, by constructing the pcDNA3-HA-hdacl, a HDAC1 eukaryotic expression vector, we were to study the interaction between HDAC1 and ERa and ERB in vitro and the effects of HDAC1 on the ERa and ERB at the protein level. So we can partly reveal the multiplication, differentiation and cancerization of lacteal gland cells.

目的 本文意在通过构建可在真核细胞中表达的重组质粒pcDNA3-HA-hdacl，研究组蛋白去乙酰化酶1(HDAC1)和雌激素受体ERα和ERβ在体外的相互结合作用，以及HDAC1对ERα和ERβ蛋白水平的影响规律，从而在一定角度揭示乳腺细胞的增殖、分化和癌变的过程，为更好地寻找治疗癌症的药物靶标打下基础。

The genes gB,gC and gD amplified from infectious laryngotracheitis virus Chinese Wanggang strain were cloned into the Eco RⅠ site of the eukaryotic expression vector pCAGGS,re-spectively. The positive recombinant plasmids were screened by transforming into E.coli DH5 α cells,which were named pCAGG-gB,pCAGG-gC and pCAGG-gD respectively after identification by restriction endonuclease analysis and sequencing analysis.

将传染性喉气管炎病毒王岗株的gB、gC、gD基因分别克隆到真核表达载体pCAGGS的 Eco RⅠ位点，经过酶切、测序分析，筛选鉴定出含有gB、gC、gD基因的重组质粒，分别命名为pCAGG-gB、pCAGG-gC、pCAGG-gD。

AIM: To construct recombinant eukaryotic expression plasmid of amastin gene of Leishmania Donovani anddetectexpressionofthegeneinNIH3T3cells.

目的： 构建杜氏利什曼原虫无鞭毛体蛋白编码基因的真核表达重组质粒pcDNA31amastin，并研究其在NIH3T3细胞中的表达。

METHODS: Amastin gene was amplified from nuclear DNA of Leishmania Donovani isolates and cloned into an eukaryotic expression vector pcDNA31.

目的： 构建杜氏利什曼原虫无鞭毛体蛋白编码基因的真核表达重组质粒pcDNA31amastin，并研究其在NIH3T3细胞中的表达。

This experiment works on:1.prepare marrow cell chromosome samples;2.take pictures of chromosome in karyon by lens-check;3.Measure the length of chromosome short arm and long arm,calculate the relative length of frog chromosome and the arm index and the position of centromere,with the leven method of outside-body karyon pattern analysis.

本实验制备了骨髓细胞的染色体标本，并且通过镜检拍下一个细胞核中染色体的照片，用leven方法进行体外核型分析，测量每条染色体的长臂长与短臂长，并计算出青蛙染色体的相对长度，臂指数和着丝粒。

Objective To establish the three-plasmid packaging cell line of the recombinant lentiviral vector encoding rat FasL gene for eukaryotic expression to meet the requisition for deep study on the effect of FasL transgene on inducing immune tolerance and protecting allografts in organic transplantation.

目的 建立真核细胞表达的大鼠FasL基因重组慢病毒载体三质粒包装细胞系统，为进一步研究转FasL基因在同种异体器官移植中诱导免疫耐受、保护移植物的作用奠定基础。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。