核粒

It was found that the coalescence of silver particles is a process of crystal nuclear growth.

实验表明，Ag粒的聚集过程也是一种晶核生长的过程。

Western-blot and immunohistochemical assay methods were employed to detect the expression of FHIT. Apoptosis and cell cycle of cell were analyzed by FCMS. Morphological changes were observed by compound and electron microscope.

然后用流式细胞仪，普通光镜及透射电镜对获得稳定FHIT蛋白表达的转染组细胞和空真核表达质粒转染组细胞及未转染的亲本细胞凋亡的状况进行了考察，另外还通过流式细胞仪检测了三组浙江大学硕士学位论文细胞生长周期的惰况。

Immunofluorescence cytochemistry and Western blot showed that angiostatin protein was expressed and secreted by GBC-SD cells in the experimental group.

1。实验得到的含血管抑素基因的真核表达质粒pcDNA3.1-angiostatin经酶切鉴定和测序证实：碱基序列和阅读框架正确。2。

RESULTS: We cloned cytosine deaminase gene and the results from gene sequencing were the same as Genbank.

将胞嘧啶脱氨酶基因亚克隆到pIRES2-AcGFP1质粒上，构建了pIRES2-AcGFP1-CD真核表达载体。

Study on bond and diamond of diamond core drill bit for reinforced concrete;2. Also glass ceramic is used as the bond of CBN grinding wheel by examining the properties of new bond .

研究了微晶玻璃中加入低熔物、成核剂后对微晶玻璃热处理曲线、微晶量及微晶体尺寸的影响，并通过测试改性微晶玻璃的软化温度和热膨胀系数对CBN磨粒的把持强度，进而将微晶玻璃用作CBN砂轮结合剂的作用作了研究，经过在数控凸轮轴磨床上的试验证明，微晶玻璃结合剂的CBN砂轮的磨削效果很好。

RESULTS: The cloned HO-1 gene in eukaryotic expression recombinant plamid pcDNA 3.1-HO-1 was confirmed by double restriction endonuclease digestion and sequence analysis.

结果：经双酶切及测序鉴定证明，正确克隆了血红素氧合酶1基因并成功构建了真核表达重组质粒peDNA 3.1-HO-1。

The results indicated that the emulsoid particles have core-shell structure obviously, its diameters are between 100 to 160nm. The glass-transition temperatures were measured by DSC, the results showed that there are three glass-transition temperatures in ACR.

通过动态光散射粒径分析仪和透射电镜对所合成的乳胶粒粒径及其分布进行了测量，借助于IR、DSC考察了所合成的ACR组成变化及其玻璃化转变温度，并通过透射电镜对所合成的ACR的核壳结构进行了验证。

Methods Eukaryotic expression plasmid pCMVCD was constructed , and identified by re2 striction endoenzyme digestion. CD gene was transfected into NSCs from new2born Wistar rats using Lipofec2tamine2000. Positive clones (named NSCs/ CD cells) were screened by G418 presence. 52Fluorocytosine (52FC) ad2ministration of different concentrations were incubated with NSCs/ CD cells. NSCs/ CD cells viability ratios were mea2 sured by MTT assay.

通过构建真核表达质粒pCMVCD ，限制性内切酶消化鉴定后，采用Lipofectamine 2000 脂质体介导法转染新生大鼠室管膜下区神经干细胞(Neural stemcells ， NSCs)，G418 筛选阳性克隆，加入不同浓度的52氟胞嘧啶(52Flourocytosine ， 52FC)，MTT比色法测定NSCs的生存率。

Objective: To construct expression vector of hTERT-hIL-18 fusion gene in eukaryotic cells and to study its biological function.

目的：以基因重组技术在真核细胞中表达人白细胞介素18(hIL-18)和人端粒酶逆转录酶融合基因，并观察其生物学功能。

Three RNAi target sites (named as Abil1, Abil2, Abil3) targeting the E3B1 (NCBI: NM-024397), an identified target sites and positive control GAPDH-A were selected. The pGenesil-1 eukaryotic expression vectors with the neoR mark and GFP green fluorescent mark were selected to construct E3B1 RNAi plasmid. The effect of RNAi targeting various sites on E3B1 genes expression was evaluated by testing the transfection efficiency with fluorescence microscope and western blot. The most effective siRNA in inhibiting E3B1 genes were screened. The most effective siRNA in inhibiting E3B1 genes screened and used to transfect the neuronal cells. After that, the inhibitor of axon growth was added and the axon growth was observed. Result: The siRNA expressing plasmids targeting the E3B1 were constructed successfully.

选择针对E3B1(NCBI： NM-024397)RNAi的3个靶位点Abil1、Abil2、Abil3和1个不针对任何mRNA的RNAi靶位点以及甘油醛－3-磷酸脱氢酶，用带有neoR选择标志和GFP绿色荧光标志的真核表达载体pGenesil-1构建E3B1 RNAi质粒，分别转染培养的神经元，在荧光显微镜下观测转染率，经G418筛选得到单一的转染细胞，并用Western blot法检测各转染组神经元E3B1蛋白的表达情况，选出具有最佳抑制效应的siRNA转染神经元，加入轴突生长抑制物，观测轴突生长情况。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。