核粒

In addition, spermiogenesis includes chromatin condensation, migration of the cytoplasm and mitochondria to the caudal pole of the nucleus, formation of a moderate axial nuclear fossa containing the proximal centriole and part of the distal centriole and presence of a short cytoplasmic canal separating mitochondria from the flagellum.

另外，精子发生过程中还包括染色质浓缩，细胞质和线粒体向细胞核的尾端迁移，在核的后端中轴位置上形成中等大小的核后凹，近端中心粒和远端中心粒的一部分嵌在核后凹之内，短的胞质内陷管将线粒体与鞭毛分隔开。

In the process of spermiogenesis, the proximal and distal centrioles translocate to the rear of the spermatid nucleus where the proximal centriole is attached, while the distal centriole elaborates the flagellum. The fluorescent centrin protein was seen to be located in basal body of flagellum. But the fluorescent spots could not be detected in the epididymal mature spermatozoa.

在精子变态分化过程中，近端和远端中心粒向核后方移动，近端中心粒附在核后膜上，远端中心粒伸出鞭毛，荧光显示Centrin蛋白位于伸出鞭毛的基部，但在成熟精子中该蛋白消失。

In this research, the ~ and F2 of the crosses between a natural mutant 慪34?with super-minute grain and 慪38?with super-large grain,慡huhui 881擲huhui 527?with middle grain size were carried out to study the inheritance of the grain shape (grain length, grain width, grain thickness and grain length/width ratio) and 1000-grain weight. The main results are summarized as below:? The F1 grain length, grain width, grain thickness and 1000-grain weight of three crosses were lay between the two parents and tended to Y34, which indicated that those grain traits were all governed by the dominant effect of Y34 and influenced by both female and male parents. The differences of F1 grain length, grain width, grain thickness and 1000-grain weight of positive and negative crosses between Y38 and Y34 indicated the existence of cytogene effects.? The broad heritabilities of major grain traits were calculated.

本研究利用一份水稻极小粒自然突变材料Y34与一份水稻极大粒材料Y38、两份常规籽粒大小材料蜀恢881、蜀恢527的杂交F_1及F_2，对主要粒形性状（粒长、粒宽、粒厚、长宽比）及千粒重进行了遗传研究，根据遗传研究的结果利用微卫星标记结合F_2群分法对控制Y34短粒性状基因进行了分子标记定位，主要结果如下：●各组合F_1粒长、粒宽、粒厚和千粒重介于双亲之间且明显偏向于小值亲本 Y34，这表明粒长、粒宽、粒厚和千粒重均主要受小值亲本显性基因的控制并同时也受大值亲本核基因的影响。Y34与Y38正反交F_1在粒长、粒宽、粒厚、千粒重等性状上存在差异，表明存在细胞质效应。

Combining ion exchange process and silicon hydrolyze process Silicon dioxide mother nuclide with a specific particle size was prepared from sodium silicate through the dropwise technique , then the large particle size , high concentration and stable silica sol was prepared . The stability of silica sol also can be improved by enwrap .

和"单质硅一步溶解法"相结合的方法，用离子交换法制备晶种，并采用滴加工艺制备一定粒径大小的二氧化硅作为母核，在催化剂和分散剂共同作用下水解单质硅的方法使二氧化硅母核颗粒进一步增长成大粒径的二氧化硅颗粒，并另外还采用水溶性高分子对纳米SiO_2粒子进行"原位表面包覆"提高其稳定性，制备了大粒径，高浓度，稳定的纳米硅溶胶。

The number of the chromosomes in Henan large tail sheep is 2n=54, the karyotypes of male and female sheep are 54, XY and 54, XX, respectively. There are 26 pairs of autosomes and 1 pair of sex-chromosome. Among 26 pairs of autosomes, 3 pairs are metacentric chromosomes, 23 pairs are telocentric chromosomes. X chromosome is the biggest subtelocentric chromosome and Y chromosome is the smallest submetacentric chromosome.

河南大尾寒羊二倍体染色体数目为2n=54，公羊核型54，XY；母羊核型54，XX.27对染色体中包括26对常染色体和1对性染色体，常染色体中有3对为中着丝粒染色体，23对为端着丝粒染色体；性染色体中，X染色体为最大的端着丝粒染色体，Y染色体为最小的中着丝粒染色体。

The ultrastructure characters of pollen grain in Brassica napus are:1There are vacuoles in cytoplasm but no starch grain in uniceelluar pollengrain.2The veget- ative nucleus of bicellular pollen grain is generally spheroidal;while the generative cell is spinde-shaped,with a big nucleus,thin layer of cytoplasmand few cell orgens,no cell wall.3In three-celled pollen grain the sperm cell is separated from the cytoplasm of vegetative cell by two layers of plasmic membrances.

Brassica napus的花粉粒，在不同发育时期超微结构的特征如下：1单胞花粉粒有一个球形的细胞核和明显的核仁，细胞质内出现液泡，缺少典型的淀粉粒。2双胞花粉粒的营养核多为球形。生殖细胞纺锤形，无细胞壁，细胞核比例大，细胞质呈薄层，细胞器少。3三胞花粉粒有一个裂片状的营养核和两个纺锤形的精细胞，精细胞无壁，以两层质膜与营养细胞的细胞质相隔。

The PSCA_3 fragment was selected for its superior expression level in eukaryotic cells.Then the sig-PSCA_3-Fc-GPI genetic fragment was cloned into pVAX1-neo-IRES-GM/B7 vector to construct the final immunological inhanced DNA vaccine pVAX1-PSCA_3-FcGB. Immunofluorescence and flow cytometry were used to confirm the expression of PSCA_3 fragment by transfected into Cos7 cell.Finally,the anti-tumor effect of pVAX1-PSCA_3-FcGB was tested in murine prostate cancer model generated by RM-1 cell line.The animal was immunized with pVAX1-PSCA_3-FcGB DNA vaccine by intramuscular injection plus electroporation,pVAX1 and pVAX1-PSCA_1-FcGB plasmid were used as control.The inhibitory effect of tumor was investigated by observion of forming time,volume and inhibition ratio of tumor.Results:DNA sequencing conformed that the heterological PSCA fusion antigen fragment which was synchronized by overlapping-extending-PCR,was consistent to design.Enzyme digestion analysis showed that the 1 to 4 copies heterological PSCA fusion antigen fragments were constructed successfully.

方法(1)检索GenBank，选择包含人主要T细胞抗原表位序列的人PSCA基因片段，应用异种化抗原设计技术，保留人T细胞抗原表位，设计异种化PSCA融合抗原片段；(2)根据核酸序列按中心模板法设计引物，应用重叠延伸PCR技术拼接合成异种化PSCA融合抗原片段基因，以PCR、限制性酶切和DNA序列测定法进行鉴定：(3)利用DNA限制性内切酶BssHⅡ和MluⅠ酶切后粘端互补的特点，采用同尾酶法构建1—4拷贝异种化PSCA融合抗原片段(PNCA_1-PSCA_4)，并将上述片段分别插入真核表达载体pCI-neo-Fc-GPI中，转染293T细胞，借助免疫荧光+流式细胞术考察插入片段表达效率，最终选定PSCA_3片段进行下一步研究；(4)将sig-PSCA_3-Fc-GPI基因片段自pCI-PSCA_3-Fc-GPI质粒上切下，插入pVAX1-neo-IRES—GM/B7载体中，构建免疫增效DNA疫苗pVAX1-PSCA_3-FcGB，并应用转染Cos7细胞+免疫荧光/流式细胞术方法鉴定其在真核细胞中的表达情况；(5)给8周龄雄性C57BL/6小鼠皮下种植RM-1细胞，制备小鼠前列腺癌模型，并采用股四头肌肌肉注射+电脉冲法(Electroporation，EP)接种DNA疫苗质粒pVAX1-PSCA_3-FcGB，同时接种pVAX1空载体质粒和pVAX1-PSCA_1-FcGB质粒作为对照，通过观察计算免疫动物的成瘤时间、肿瘤体积和抑瘤率，来评价该DNA疫苗在小鼠体内的抑瘤效果。

HTERT is expressed in cancer cell lines despite promoter DNA methylation by preservation of unmethylated DNA and active chromatin around the transcription start site.

人端粒酶逆转录酶是一种核糖核蛋白酶，由端粒RNA和端粒酶蛋白组成的一种特殊逆转录酶，在激活端粒酶的过程中起限速作用，能以自身RNA为模板合成端粒序列以稳定端粒长度使细胞获得永生，也是人类端粒酶的活性中心，其表达是端粒酶活化的必须前题［2～4］。

By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length P1, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length P1, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3. 1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus , which were expressed in BHK-21 cells, were confirmed by sandwich-ELISA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3. 1/IFN which includes the gene IFN-α of cattle. Subsequently, Recombinant plasmids were injected to cattles with or without pcDNA3. 1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies titers were analyzed by micro-neutralization assay.

为研制带有O型口蹄疫病毒(Foot-and-Mouth Disease Virus，FMDV)China99株结构蛋白基因及多个非结构蛋白基因的DNA疫苗，本研究通过定点突变方法和PCR扩增方法，获得包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C以及部分2B、3B编码基因的片段P12X3C和包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C、3D以及部分2B、3B编码基因的片段P12X3C3D，将获得的基因片段直接/酶切后与同样处理的真核表达质粒连接，分别得到重组质粒pcDNA3.1/P12X3C和pcDNA3.1/P12X3C3D、pTARGET/P12X3C3D；对重组质粒进行序列测定、分析，并将重组质粒分别转染BHK-21细胞，通过双抗体夹心ELISA方法和间接免疫荧光标记方法检测细胞中FMDV抗原的表达，用电子显微镜观察病毒空衣壳的组装；为评价重组质粒作为DNA疫苗对实验动物及本动物的免疫效果，将重组质粒经肌肉注射方法接种豚鼠，并与酵母表达的纯化FMDV China99株3D蛋白及带有牛α干扰素的真核表达质粒pcDNA3.1/IFN分别/同时免疫，第二次免疫后第三周豚鼠攻以1OOID〓或1000ID〓的O型FMDV China99株；随后将质粒pcDNA3.1/P12X3C、pcDNA3.1/P12X3C3D与带有牛α干扰素的真核表达质粒pcDNA3.1/IFN同时免疫牛，三周后经牛舌皮攻以10〓ID〓的O型FMDV China99株。

By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length PI, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length PI, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3.1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus, which were expressed in BHK-21 cells, were confirmed by sandwich-ELlSA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P 12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3.1/lFN which includes the gene IFN-a of cattle. Subsequently, Recombinant plasmids were injected to catties with or without pcDNA3.1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies liters were analyzed by micro-neutralization assay.

为研制带有O型口蹄疫病毒(Foot-and-Mouth Disease Virus，FMDV)China99株结构蛋白基因及多个非结构蛋白基因的DNA疫曲，本研究通过定点突变方法和PCR扩增方法，获得包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C以及部分2B、3B编码基因的片段P12X3C和包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C、3D以及部分2B、3B编码基因的片段P12X3C3D，将获得的基因片段直接／酶切后与同样处理的真核表达质粒连接，分别得到重组质粒pcDNA3.1／P12X3C和pcDNA3.1／P12X3C3D、pTARGET／P12X3C3D；对重组质粒进行序列测定、分析，并将重组质粒分别转染BHK-21细胞，通过双抗体夹心ELISA方法和间接免疫荧光标记方法检测细胞中FMDV抗原的表达，用电子显微镜观察病毒空衣壳的组装；为评价重组质粒作为DNA疫苗对实验动物及本动物的免疫效果，将重组质粒经肌肉注射方法接种豚鼠，并与酵母表达的纯化FMDV China99株3D蛋白及带有牛α干扰素的真核表达质粒pcDNA3.1／IFN分别／同时免疫，第二次免疫后第三周豚鼠攻以100ID_(50)或1000ID_(50)的O型FMDV China99株：随后将质粒pcDNA3.1／P12X3C、pcDNA3.1／P12X3C3D与带有牛α干扰素的真核表达质粒pcDNA3.1／IFN同时免疫牛，三周后经牛舌皮攻以10~4ID_(50)的O型FMDV China99株。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。