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To sum up, the research for the first time ascertained INA species in apricot trees and their activities by using the rational method to imatate the temperature dropping process in frost nights--computer temperature controlling with semiconductor cooling and artificial frost box with self-recording. Studies were given to responds of INA to freezing temperature of floral organs, biochemical cold resistance parameters, contents of ABA and IAA, chlorophyll fluorescent parameters of young fruits and tissue structure and ultrastructure of apricot. The mechnism of INA induced frost injury of apricot was found out. And it was demonstrated that INA is a critical factor in the inducing and aggravation frost injury of apricot floral organs.

综上所述,本研究采用能够准确模拟自然界霜夜降温过程的较为理想的方法——半导体致冷电脑控温和自动记录的人工霜箱,首次查明了10个杏树品种上INA细菌种类和冰核活性,从INA细菌对杏树花器官过冷却点、抗寒生理生化指标、激素ABA、IAA含量、幼果叶绿素荧光参数以及组织结构和超微结构的影响等方面进行了系统深入的研究,阐明了INA细菌诱发杏树霜冻害的机理,证明了INA细菌是诱发和加重杏花霜冻害的重要因素。

Purified protein samples were separated by 2-DE with 17 cm IPG strips, and the protein spots were detected by fast silver staining. Six protein spots were randomly excised from the silver stained gel for primary analysis by MALDI-TOF MS. Four proteins were identified, and they were 1-aminocyclopropane-1-carboxylate synthase 2, ribulose-1, 5-bisphosphate carboxylase/oxygenase large subunit, F11O4.2 and Rubisco small subunit.

根据预实验结果,选用17 cm IPG预制干胶条对纯化的蛋白样品进行2-DE实验,采用快速银染法进行染色,随机挖取银染胶上6个蛋白点进行MALDI-TOF MS分析和数据库检索,鉴定了3个蛋白,它们是:1-氨基环丙烷-1羧酸合酶2,核酮糖-1,5-二磷酸羧化酶/加氧酶大亚基和F11O4.2。

The uniqueness of solution of field point, inside a convex region due to singular source with kernel function decreasing with distance increasing, outside-region-distribution such that the boundary condition expressed by the response of the source is satisfied, is proved by using the condition of kernel function decreasing with distance increasing and an integral inequality.

应用奇源解的核函数沿距离递减的条件和积分不等式,可证这类奇源在凸域外分布只要满足以它产生的响应来表达的边界条件时,所引起相应的域内场点解是唯一的。

In general the precise calculating method of stability check of the slightness hydraulic cylinder is an outdo equation.

利用台劳级数,把液压缸稳定性校核精确解超越方程的各项在较为理想的点展开,去掉其高阶项,仅保留一次项,得到方程的近似解,并证明其安全性。

Cerebral hemorrhage rat models were established via injection of autologous arterial blood in caudate nucleus. Two days after modeling, 5 μL BrdU-labeled human embryonic NSCs suspension was transplanted at four points surrounding hematoma cavity in the rats. After 1 and 2 weeks, rats were sacrificed. Adjacent sections were doubly stained by BrdU/microtubule-associated protein 2 (MAP-2) and BrdU/glial fibrillary acidic protein.

通过注射自体动脉血到尾状核制作大鼠脑出血模型,出血后2 d将标有5'-溴脱氧尿嘧啶的人胚神经干细胞悬液移植到血肿腔周围的4点,1,2周后处死大鼠,相邻脑组织切片行5'-溴脱氧尿嘧啶/微管相关蛋白2和5'-溴脱氧尿嘧啶/胶质纤维酸性蛋白免疫组织化学双染。

METHODS: Cerebral cortex cells of 8-week aborted human fetus were harvested and cultured in vitro to obtain human embryonic NSCs. Cerebral hemorrhage rat models were established via injection of autologous arterial blood in caudate nucleus. Two days after modeling, 5 μL BrdU-labeled human embryonic NSCs suspension was transplanted at four points surrounding hematoma cavity in the rats.

通过注射自体动脉血到尾状核制作大鼠脑出血模型,出血后2 d将标有5'-溴脱氧尿嘧啶的人胚神经干细胞悬液移植到血肿腔周围的4 点,1,2周后处死大鼠,相邻脑组织切片行5'-溴脱氧尿嘧啶/微管相关蛋白2和5'-溴脱氧尿嘧啶/胶质纤维酸性蛋白免疫组织化学双染。

Aim: To study the effect of the location of computer assistant stereotactic operation combined with the electrophysiological stimulation in thalamotomy and pallidotomy to treat the Parkinson disease.

目的 :研究计算机辅助立体定向脑内靶点定位与术中电生理刺激射频结合确定丘脑腹外侧核、苍白球腹后外侧部在治疗帕金森病中的作用。

Objective To evatuate the effect of the computer assistant stereotactic operation combined with the electrophysiological-stimulation in thalamotomy and pallidotomy to treat the parkinson disease.

目的研究计算机辅助立体定向脑内靶点定位与术中电生理刺激结合确定丘脑腹外侧核、苍白球腹后外侧部在治疗帕金森病中的作用。

Methods Orientated by CT,108 patients underwent pallidotomy and thalamotomy for Parkinson′s disease by means of stereotaxy microelectrode recording as intraoperation target localization.

采用 CT定位,立体定向微电极细胞电生理记录技术进行术中靶点监测,对 10 8例帕金森病人的苍白球和丘脑腹外侧核进行射频毁损术治疗。

By using λDNA labled with 32 P as probe,unlabled λDNA as positive check,dot blottings of nuclear DNA and chloroplast DNA of receptor 814527,D type sterile line and its hybrid F 1 with Lumai 14 were carried out.

用32 P标记的λDNA作为探针,对受体 81 45 2 7、D型不育系及其与鲁麦 1 4号的杂种F1 核DNA和叶绿体DNA进行点杂交。

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