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Through all this test analyse Pathogenicity and Immunological status of ALV-J Inner Mongolia strain, the result showed the infected chickens began to have pathological change of Myelcytomatosis at 3-week: the main pathological changes of acidophile myelocyte were found in bone marrow and between cardiac muscle fibers,and there were many granules in the hyalotome.This kind of oncocyte proliferated in every tissue at different amount, but the cells that proliferated in bone marrow, cardiac muscle fiber, oarium were much more than other tissue, and they often developed neoplasma. At same condition,there were no abnormalty advent in control group, In immunological aspect: the increase of lymphokine and ANAE positive cells showed cell-mediated immunity was strengthend during antineoplastic infection; the emergence of ALV-J antibody of ELISA suggest there was humoral immunity exist at the same time.

结果显示:脾脏PCR检测结果表明1、2两实验组均有阳性病例出现,而对照组全部为阴性,说明实验鸡体内已有ALV-J内蒙株感染;接种病毒后3周龄开始出现骨髓细胞瘤病的病理变化:肝脏、脾脏有程度不同的肿大,镜下观察,骨髓内最早出现了与骨髓细胞相似、形态基本一致的大型圆形或椭圆形、胞核较大多为椭圆形、胞浆内含有大量嗜酸性圆形颗粒的髓细胞样瘤细胞增生,随后通过血流瘤细胞可转移到肝脏、心肌、卵巢或睾丸等组织并形成肿瘤性病灶,而对照组没有出现明显病变;免疫状态方面:以脾脏淋巴因子和ANAE阳性细胞增多说明细胞免疫在抗肿瘤感染过程中增强,ELISA检验实验鸡抗体出现阳性的结果表明同时也存在着体液免疫。

The results support the hypothesis of Polyploid gigantism and show the effect of polyploidy on nuclear size.

研究结果如下:一、三倍体牡蛎鳃上皮细胞核长轴、外套膜与唇瓣结缔组织中血淋巴核径的大小明显大于二倍体(P<0.001),二倍体牡蛎直肠上皮细胞的高度与三倍体差异不明显(0.05<P<O.1)。

Results In the medium and high dose F0 groups, it was observed that the atrophy and incrassation of seminiferous tubule, decrease of spermatogenesis, hyperplasia of interstitial tissue, especially in high dose groups spermatozoon abnormality and nucleolus concentration in the rats testis after DU ingestion for 14 months. The changes became more severe with the prolongation of DU ingestion. Such changes occurred in filial rats (F1) after DC ingestion for 5 months. In the medium and high dose F0 groups, it was observed that a little atrophy of kidney glomerulus, hyperplasia of interstitial tissue after DC ingestion for 14 months, and kidney glomerulus fibrosis happened after DC ingestion for 20 months, such changes occurred in filial rats (F1) after DC ingestion for 5 months In the medium and high dose F0 groups, splenic germinal center and periarterial lymphatic sheaths were hyperplasia , companies with lymphopoiesis after DC ingestion for 7 months, splenic white pulp became more small and sparse after DC ingestion for 20 months.

结果 F0代的中、高剂量组大鼠摄入贫铀14个月后可见雄性的精曲小管萎缩,管壁增厚呈空虚网状,生精细胞层次减少,间质细胞增生,但仍见有精子生成;高剂量组可见到精子呈异型性改变,细胞核浓缩深染,且随着摄入时间延长改变愈趋明显;F1代大鼠摄入贫铀5个月后就有上述改变且更为严重。F0代中、高剂量组大鼠摄入贫铀14个月后肾小球轻度萎缩,间质增生明显,20个月时肾小球萎缩纤维化;F1代大鼠摄入贫铀5个月后就有上述改变。F0代中、高剂量组摄入贫铀7个月时脾脏生发中心和淋巴鞘增生,淋巴母细胞增生活跃,20个月时脾小体减少,生发中心稀疏;F1代大鼠摄入贫铀早期和晚期有类似改变。F0和F1代高剂量组摄入贫铀早期肝脏有炎症细胞浸润,晚期骨髓有核细胞减少,脂肪细胞增加。

Vestibule; temporal bone; bony labyrinth; inner ear; perilymph; angular acceleration; hair cells; stereocilia; crista ampullaris; ampulla; cristae; cupula; vertigo; VOR; vestibulo-ocular reflex; vestibular nerve; vestibular nuclei; vestibular ganglion; equilibrium

前庭;颞骨;骨迷路;内耳;外淋巴;角加速度毛细胞;静纤毛;壶腹嵴;壶腹;嵴;吸盘;眩晕;VOR;前庭眼球反射;前庭神经;前庭神经核;前庭神经节;平衡

The results show that DNA content and pleomorphism of the nucleus of the cancerous cell are of two prognostic factors.

并探讨了胆管癌细胞核形态学与其TNM分期、组织病理学分级、肿块大小、浸润性和淋巴转移等生物学行为的关系。

Based on the histochemistry observation, there appears two types of cell in the lymphoid tissues of sacculus rotundus in the 1st day: A type showing large, pale, round or oval shapes, has large and bright nuclear, but little cytoplasm.

组织形态学观察发现,在1d兔圆小囊淋巴组织中主要可见两种类型的细胞:其中Ⅰ型细胞体积大、着色浅,核大而空亮。

In the present paper, the induction characteristic of lymphoblastoid, in-terferon in vitro is described. Results indicate that Namalwa cell line possesses a higher interferon-inducing ability than Raji and Yu-Nu cell lines. NDV B1 strain, F strain and Sendai virus can induce more interferon than BB1 virus and poly 1 : C. Priming of pretreating cells with homologous interferon or superinduction did not effect interferon production. The optimal cell concentration for interferon induction is about 106 cells/ml...

本文研究了类淋巴母细胞干扰素的诱导特性:Namalwa细胞系的干扰素诱生能力大于Raji和余奴系,而诱生剂中以NDV和仙台病毒诱生干扰素的能力较大,但"起动"和超诱导均无作用;诱生干扰素适宜的细胞浓度为10~6/毫升;增殖期的Namalwa细胞诱生能力较大;黄芪和5'-溴脱氧尿嘧啶核苷对Namalwa细胞干扰素的诱生系统有明显刺激作用,在最适条件下粗制干扰素平均滴度可达40,000单位/毫升。

In the present paper, the induction characteristic of lymphoblastoid, in-terferon in vitro is described. Results indicate that Namalwa cell line possesses a higher interferon-inducing ability than Raji and Yu-Nu cell lines. NDV B1 strain, F strain and Sendai virus can induce more interferon than BB1 virus and poly 1 : C. Priming of pretreating cells with homologous interferon or superinduction did not effect interferon production. The optimal cell concentration for interferon induction is about 106 ce...

本文研究了类淋巴母细胞干扰素的诱导特性:Namalwa细胞系的干扰素诱生能力大于Raji和余奴系,而诱生剂中以NDV和仙台病毒诱生干扰素的能力较大,但"起动"和超诱导均无作用;诱生干扰素适宜的细胞浓度为10~6/毫升;增殖期的Namalwa细胞诱生能力较大;黄芪和5'-溴脱氧尿嘧啶核苷对Namalwa细胞干扰素的诱生系统有明显刺激作用,在最适条件下粗制干扰素平均滴度可达40,000单位/毫升。

Results Revealed the pathognomonic changes:the mantle cell zone were remarkably wide,but germinal center became small,the nucleous form of it were irregular,and the structural changes of lymph node also slight.

结果 发现其以淋巴套细胞显著增宽而生发中心明显缩小,瘤细胞小,核形轻度不规则和原有淋巴结结构破坏较轻为其形态特征。

Pathological observations showed that the bone marrow and spleen of non-treated IMAA mice demonstrated typical aplastic anemia pattern, including bone marrow failure, marked splenatrophy, but the bone marrow and lymphoid tissues in the IMAA mice of irradiation-treated group were recovered to normal at day 28 after treatment.

结果表明:①IMAA组小鼠活存率为12.5%,平均存活时间为27.4 13.4天,照射治疗组活存率为100%,平均存活时间60天以上,单纯照射组无死亡;②外周血白细胞数:IMAA组呈进行性下降,直至死亡,照射治疗组第10天与免疫再障组相似,以后开始缓慢回升,基本上达到治疗前水平;③红细胞比容:未治疗IMAA组于14天后呈进行性降低,至第35天比实验前减低2/3,照射治疗组与单纯照射组一样,第14天有轻度减低随后升高,至35天接近正常;④骨髓有核细胞数:IMAA组呈进行性减低,无恢复趋势,照射治疗组于一过性减低后迅速增加,第28天达到正常水平;⑤骨髓和淋巴组织病理形态观察:IMAA组小鼠呈典型再生障碍性贫血病理改变,骨髓造血衰竭,脾脏明显萎缩,而照射治疗组于第28天骨髓和脾淋巴脏组织基本上恢复正常。

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