核染质

So in order to get recombinant NT-3, the rat NT-3cDNA was reconstructed in a mammalian expression vector and transfected into incasing CHO cells by liposome-mediated method. The clones of the cells transferred with rNT-3cDNA were selected by G418. The Chinese hamster ovary cell line stably producing recombinant rat NT-3 was established.

因此，为了获得基因重组NT-3，研究其生物效应，本文成功构建了重组大鼠NT-3基因真核生物细胞稳定表达载体，并利用脂质体介导，转染进CHO细胞；经G418筛选，建立了中国仓鼠卵巢细胞系，稳定表达重组大鼠NT-3。

RESULTS After treatment with CDDP, the cells became smaller and condensed. Their chromatin clinged to the periphery of the nuclear.

结果 细胞经顺铂处理后浓缩、深染、致密的染色质沿核膜下凝集、有凋亡小体形成。

Cellular transfection and localization observation were performed to eucaryotic cells under mediation with liposome.

脂质体介导真核细胞进行转染并细胞定位观察。

OBJECTIVE:To study the effects of eukaryon expressed adiponectin on the glycogen synthesis and glucose oxidation in skeletal muscle cell strain C2C12 myotubes by transfecting plasmids carrying mouse adiponectin.

目的：通过转染带小鼠脂联素基因的质粒，观察真核表达的脂联素对骨骼肌细胞株C2C12肌小管葡萄糖氧化和糖原合成的影响。

For the purpose of constructing mammary gland specific expressional vector, we use the updream regulation sequence of bovine BLG to regulate lacS gene to express in mammary gland. Positive clone cells are selected and purified by the expression Neo gene and report gene EGFP. So the cells can be used as sources to create transgenic animals by nuclear transfer techniques.

本研究以牛β-乳球蛋白基因上游调控成分作为启动子，嗜热菌糖苷酶基因lacS为目的基因，构建了lacS基因乳腺特异表达载体，并通过脂质体介导法转染体外培养的牛胎儿成纤维细胞，借助新霉素抗性基因和增强型绿色荧光蛋白报告基因来筛选、纯化阳性克隆细胞，为利用核移植技术制备转基因动物提供细胞来源。

Lipofectamine was used to transfect pVMm into C2C12 and the expression of mMCP-1 was detected by ELISA.

以脂质体将pVMm转染真核细胞系C2C12，ELISA分析mMCP-1的表达情况。

AIM To construct the expression vector of endo-statin gene and transfect it into C6cell,and to discuss its an-tiangiogenesis activity.

目的 构建endostatin基因的真核表达载体，并将其转染C6脑胶质瘤细胞，探讨其体外抑制血管内皮细胞生长的活性。

MethodsAfter construction of a fulllength human βNGF expression vector, human cholangiocarcinoma cell QBC939 was transfected with pcDNA3.0NGF.

方法构建βNGF的真核表达载体pcDNA3.0NGF，经酶切鉴定后，采用脂质体lipofectamine转染人胆管癌细胞株QBC939。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。