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Due to the breakthrough of stained technology in 1970s, the band-pattern of chromosome provides material visual information for cytogeneticist, then analyses the composition of genome in a cell and measures the features of the equipments for karyotyping.In order to meet the demands of clinical hospital, a chromosome analysis system based on PC is developed.

由于70年代标本染色技术的突破(取代了此前的均匀染色方法,产生了染色体的带型特征),染色体的带型模式为细胞遗传学家提供了实质性的视觉信息,从而可以进一步分析细胞的基因组的合成以及进行自动核型分类仪器中的特征测量。

Proliferative index34.0%, mitotic index2.74%(1.20%~4.80%), apoptotic index1.30%~6.90%, chromosome analysis mainly 46 (44~54/nucleus) and DNA distribution in diploidy were calculated and described.

增殖指数34.0%,分裂指数2.47%(1.20%~4.80%),凋亡指数1.30%~6.90%,核染色体众数46条为主(44~54条)和细胞周期DNA呈2倍体核型分布。

The baculovirus expression system, utilizing the strong polyhedrin gene promoter of the Autographa californica nuclear polyhedrosis virus , has become an important tool for protein production in the field of molecular biology.

由于核型多角体杆状病毒的多角体蛋白不是病毒复制必需的蛋白,利用转录多角体蛋白基因的强启动子可以高效表达外源蛋白,包括原核和真核基因编码的蛋白。

Software and results were saved in EXCEL for consequent statistic operation and obtaining chromosome patterns. 4 indexes were chose, such as chromosome karyotype, C-band pattern, chromosome behavior and B chromosome for comparative analyses, and adopted SPSS to reconstruct dendrogram. Combined with studies on insect morphology and insect geography, we made a detailed analysis and speculation on the relationship of taxa concerned. Finally we got the conclusions as follows: 1. The number of chromosomes in grasshoppers of Oedipodiae is 2n 〓=23=22+XO. All chromosomes are telocentric chromosomes. Most of the genome formulas are 3L+6M+2S+X and 3

论文选取染色体核型、C-带带型、染色体行为和B染色体这4个方面的指标进行比较分析,运用SPSS系统分析软件建立表达各属和各种之间亲缘关系的系统树,并结合昆虫形态学和昆虫地理学等方面的研究成果,对其亲缘关系进行了比较详细的分析和推测,得出以下结论:一、斑翅蝗科蝗虫的染色体数目都稳定在2n〓=23条,性别决定机制是XO型,染色体均为端部着丝粒,染色体组式以3L+6M+2S+X和3L+7M+1S+X这两种类型为主,即3对大型染色体,6~7对中型染色体和2~3对小型染色体,X染色体以中型为主,在染色体组中主要排列为4~7位。

Objective To investigate the relationship of human immunoglobulin and complement with both the type and titer of antinuclear antibody.

目的探讨抗核抗体核型及滴度与免疫球蛋白和补体的关系。

The embryological studies of strawberry showed that the ovule of strawberry were hemitropous, the ovule had the obvious hypostace structure, the type of its embryo sac was Polygonum, the embryo sac was long and narrow, the type of development of embryo was the Geum type of the Asterad type, the endosperm was Nuclear type and the somatic embryo sacs happened The integral transparency technique combined with the technique of microdissection was used in this experiment The combination was an innovation in the techniques of section.

对草莓胚胎学的研究结果表明:草莓胚珠横生、胚珠具明显的承珠盘结构、胚囊发育为蓼型、胚囊狭长、胚胎发育为紫菀型水杨梅类型、胚乳核型、并有体细胞胚囊的产生。试验中采用了多种制片方法,其中将整体透明技术和显微解剖技术相结合的方法是在制片技术上的一次创新。

Methods : The molecular genetics analysis of peripheral blood karyocyte and orchi- biopsy spermatogenous cell in two patients with chromatosome caryotype abnormity in peripheral blood screened routinely

结果 核型为45,X0/46,XY,del(q11.21);45,X0/46,XY,del(q11.23)二位患者外周血有核细胞和睾丸活检精原细胞AZF区域15个标签位点分别只扩增出SY84和SY86;SY86,其它区域位点均未检测到。

Results The time of villiform cell culture was obviously related to the kinds of digestive enzyme, but not to the number of karyotypes. The villiform cells of 68 specimens (93.15%) were cultured successfully and 30 abnormal karyotypes were tested.

结果 经不同消化酶处理后,绒毛细胞培养时间具有显著相关性,但与核分裂相的数目无相关性。73例中,68例培养成功,成功率93.15%,检出异常核型30例。

In this study, the chromosome karyotype analysis and RT-PCR method are employed to explore the taxonomic characteristic of the planarians.

通过核型分析和RTPCR技术,从核学和分子生物学水平对三角涡虫的分类学特征进行研究。

The present paper report the karyotype of four nemerteans and nuclear DNA content s of eight nemerteans.

本文研究了4种纽虫的核型和8种纽虫的核DNA 含量,为纽形动物的细胞遗传学研究提供参考。

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