核型

The karyotypes and the Giemsa-C banding patterns of Aegilops tauschii (85A-34) and Hordium vulgare (H2) and regenerated plants from embryo callus of hybrids between Ae.tauschii and H.vulgare (2n=28) were confirmed by karyotype analyzing and Giemsa-C banding technique in this study, thus proved that the regenerated plants from embryo callus of hybrids between Ae.tauschii and H.vulgare (2n=28) were spontaneously chromosal doubling amphidiploid.

本研究通过染色体组型分析和Giemsa--C带显带技术，确定了节节麦（85A85A-34）、栽培大麦（H2）、节节麦×大麦杂种胚再生植株(2n=28)的染色体组型和Giemsa--C带核型，证明了节节麦×大麦杂种胚再生植株(2n=28)为自发加倍的节节麦－大麦双二倍体。

It was known that ORF of SeMNPV egt was 1572bp long and encodes 523 amino acids through DNAstar software analysis. TATA boxes lies at 48~53 and 68~72bp of the 5'-nonencoding region upstream of a translational start site. Comparing with seven kinds of nuclear polyhedrosis virus and one kind of granulosis virus, it indicated that the SeMNPV egt gene is the highest match the Agrotis setegum nuclear polyhedrosis virus.It has 75% nucleotides and 79% amino acid identity.

采用DNAstar软件分析，与7种核多角体病毒及1种颗粒体病毒egt基因的同源性比较显示，SeMNPV egt基因与核多角体病毒egt序列同源性较高，其中与黄地老虎核型多角体病毒同源性最高，核苷酸和氨基酸序列同源性分别为75%和79%。

A pair of primers producing 614bp amplification fragment were designed based on the sequence of the C\|polyhedrin gene of Bombyx mori CPV,and the expected amplification products were obatined when genomic dsRNAs isolated from purified BmCPV,DsCPV and Lymantria dispar CPV were used as templates.Genomic dsRNAs isolated from Heliothis armigera CPV and DNAs from Lymantria dispar nuclear polyhedrosis virus and DNAs from midgut tissue of healthy Dendrolimus spectabilis larva did not yield any amplification products.The detection limit of purified DsCPV genomic dsRNAs was 1.0pg.

依据家蚕质型多角体病毒质型多角体蛋白的核苷酸序列设计一对引物，从纯化的DsCPV\，BmCPV和舞毒蛾质型多角体病毒的基因组dsRNA可成功地扩增出长614bp的目的片段，从提取的健康松毛虫幼虫肠组织的DNA、舞毒蛾核型多角体病毒基因组核酸、以及棉铃虫质型多角体病毒基因组核酸，未能扩增出目的片段。

Methods Endogenous EB1 mRNA and protein levels in embryonic cells of trophonema from artificial abortion and spontaneous abortion were compared, using fluorescence quantitative PCR, immunohistochemistry and western blot, respectively.

采用荧光定量PCR技术检测EB1基因在人工流产绒毛核型分析正常的胚胎细胞和自然流产绒毛核型分析异常的胚胎细胞中的表达差异；免疫组织化学方法检测EB1蛋白在两者中的表达情况，并用Western blot加以验证。

Methods Endogenous EB1 mRNA and protein levels in embryonic cells of trophonema from artificial abortion and spontaneous abortion were compared, using fluorescence quantitative PCR, immunohistochemistry and western blot, respectively.

结果 EB1基因mRNA拷贝数/GAPDH基因mRNA拷贝数的均值在人工流产绒毛核型分析正常的胚胎细胞中为0.01004±0.008223，在自然流产绒毛核型分析异常的胚胎细胞中为0.1509±0.0633。

Results:Thirty-six cases (40.4%) had clone chromosomal abnormalities and 12 categories of major abnormalities were found, five specific abnormalities of 12 were seen in 25 cases,accounting for 69.4% of all patients with karyotypic abnormalities.

结果：89例M4患者中，异常染色体检出率为40.4%(36/89)，共12种主要异常核型，其中5种为特异性染色体异常，见于25例患者，占核型异常患者的69.4%。

Objective: The aims of our research are ① to filter candidate Wnt genes which are important for maintenance of normal hESCs or resulting in karyotypic change through analysis of Wnt genes of differential expression in human and mouse feeder cells and hESCs during culture of normal or abnormal karyotype hESCs.② to research the function of Wnt9a that RNAi for human Wnt9a were performed.

研究目的：对核型正常和异常的人类胚胎干细胞在体外培养过程中Wnt基因在饲养层和hESCs中的差异表达分析，分别筛选出可能促进hESCs正常增殖和引起核型发生变化的候选Wnt基因，并对其中的Wntga候选基因进行RNAi研究，了解其生理功能；在小鼠睾丸或生殖域中克隆与生精相关的新基因，并对其功能进行初步研究。

Karyotypes of root tip chromosome showed that the arm volume ratio, the mean relative volume and idiogram were identical before and after improving seed vigor, so the pattern of chromosome did not change. But we couldnt confirm whether the pattern of other separation dates and the inner structure varied in this experiment, such as gene mutation.

根尖染色体核型分析结果，甘蓝种子在活力提高前后染色体的臂比值相同，相对长度相同，表明活力提高后染色体在形态及核型上无明显变化，而对于除中期外的其它分裂期染色体的形态特征以及染色体内部结构有无明显变异在本试验条件下尚不能确定。

Karyotype formula of Gossypium hirsutum L.was 2n=4s=52=30m+20m (4SAT)+2st (2SAT); and its absolute lengths of chromosomes averaged 3.40μm; arm ratio differed from 1.24 to 4.02,with the mean value of 1.77; three pairs of satellite chromosomes; the ratio of the longest to the shortest chromosomes was 2.36; and the karyotype belonged to 2B in Stebbin's classification.

陆地棉的核型公式为2n=4x=52=30m+20sm(4SAT)+2st(2SAT)；染色体绝对长度平均3.40μm；臂比值最大与最小的分别是4.02和1.14，平均1.77；随体3对，分别位于第12、19和26对同源染色体的短臂上；最长与最短染色体的比值为2.36；其核型属于Stebbin的2B类型。

Results In these 68 normal and 32 Down's syndrome samples, the result by QF-PCR are in accord with the cytogenetics analysis.

结果：细胞遗传学分析确诊的68例正常核型和32例唐氏综合征标本中，荧光定量PCR快速诊断结果均与染色体核型分析结果一致。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。