核型

A new eucaryotic expression system of recombinant PoIFN-γ was also constructed so that the denaturalization and refolding process is avoided. The target gene coding PoIFN-γ2 mature protein was subcloned into expression vector, transformed into Pichia pastoris, The His〓 Mut〓 phenotype transformants were screened, fermented in flasks and induced by 1％methanol. The expressed product in culture solution and sedimentation have antiviral activity on VSV and immunological activity proved by indirected immunofluorescence antibody and Dot blotting assay.

为了构建可对表达产物修饰、加工的真核表达系统，使表达产物具有自然干扰素的活性，无需变性、复性，我们将PoIFN-γ2成熟蛋白基因连接酵母整合表达载体，电转化毕赤酵母，筛选了His〓Mut〓表型转化子，摇瓶培养，1％甲醇诱导表达，培养上清和菌体裂解上清均有抗VSV病毒活性，经间接免疫荧光抗体检测和Dot blotting鉴定，重组酵母菌诱导表达产物具有免疫活性。

Secondary growth of silicalite-1-seeded industrial CuO-ZnO-Al2O3 in alkaline solution with silica and alumina source produced intergrown ZSM-5 zeolite shells. After the shells were changed into HZSM-5, we compared the catalytical performance of the core/shell composite catalyst with a non-core/shell structured composite catalyst with the same component.

铜锌铝催化剂（CuO-ZnO-Al2O3）是工业上制备二甲醚的良好催化剂，我们将其表面预先包覆纳米沸石silicalite-1晶种，水热环境下二次生长后吸收硅源、铝源后形成ZSM-5膜，转换成氢型后形成CuO-ZnO-Al2O3/HZSM-5的核/壳结构复合催化剂。

Expresion of FasL was found mainly in the cytoplasm of liver-infiltrating mononuclear cells,these positive cellswere found particularly at the advancing edges of piecemeal necrosis,bridge necrosis and in the intralobular.

以免疫组化法研究Fas配体在丙型肝炎病人肝组织内的表达情况，发现在碎屑样坏死、桥样坏死及灶性坏死区浸润的单个核细胞有明显的FasL表达，阳性率为41.2％。

With confocal laser scanning microscopy, it was found that the mutant type p53 protein was located in the cytoplasm while the wild type p53 protein was located in the karyon.

用激光共聚焦显微镜观察到突变型p53蛋白定位于胞浆，而野生型p53蛋白则定位于胞核内。

The dissertation mainly consists of two parts:1. The Reaction Chemistry of Alkynes under Palladium Catalysis Part 1 studies the reaction α,β-unsaturated carbonyl compounds with alkenylpalladium intermediate generated by nucleopalladation, and discovered 1 the Pd-catalyzed tandem addition reaction of halides, alkynes and α,β-unsaturated carbonyls,2 the four-component tandem addition reaction of halides, alkynes and α,β-unsaturated carbonyls, 3 the tandem intramolecular oxypalladation-conjugate additionreaction of alkynoic acids with α,β-enals. These new reactions provide notonly efficient highly stereoselective methods for the synthesis of γ,δ-unsaturated,polyunsaturated carbonyl compounds and γ-lactonic aldehydes, but also thebasic transformations for a number of C-C bond formation reactions which can be usedto design synthesis of many acyclic, cyclic or polycyclic compounds.

本论文的主要工作分为两个部分：一、钯催化下炔烃的反应化学在本小组前人工作的基础上，我们详细研究了炔烃亲核钯化产生的烯基钯中间体与α，β-不饱和羰基化合物的反应，发现两价钯催化下卤离子-炔烃-α，β-不饱和醛酮的串联加成反应、卤离子-炔烃-α，β-不饱和醛酮的西组分串联加成及炔酸分子内氧钯化-串联Michael型加成反应；由此发展了γ，δ-不饱和醛酮、多不饱和羰基化合物和带有醛酮基侧链的γ-丁内酯衍生物的高立体选择性合成方法，这一过量卤离子存在下的烯基钯对α，β-不饱和羰基化合物共轭加成反应的发现，为大量碳-碳键形成反应的实现提供了基础，使我们能够从这一基本反应出发，设计和开发多种分子内、分子间的碳碳成键反应，以好的立体、区域选择性组建开链、环状及多环类型的化合物。

When the MCA trunk is occluded at its origin, and all of the lenticulostriate arteries are involved in ischemia (type 1 occlusion), hypodensity usually extends to the entire lentiform nucleus.

当大脑中动脉主干的起始段发生闭塞、造成所有豆纹动脉均受累缺血（1型大脑中动脉闭塞）时，通常整个豆状核区都会出现低密度

The determination demonstrated that the anionic polymerization of ε-caprolactam under 230℃ leaded to a mesomorphous form of PA6; the EPDM had heterogeneous nucleation effect on the crystallization of dispersed PA6 phase; in situ polymerization could further reduce crystallization enthalpy of PA6 component on the level of reactive compatibilization.

研究表明，230℃下进行阴离子聚合得到的PA6为β-介晶型；EPDM对这种分散在橡胶相中的PA6的结晶具有异相成核作用；原位聚合可在反应增容的基础上使得共混物中PA6的结晶焓进一步降低。

In order to better understand their phylogenetic relationship and identify the uncertain species, the sequences of the small subunit ribosomal RNA (ssurRNA, 16 S rRNA) genes of nine microsporidia infectious to the silkworm, Bombyx mori, were determined.

微孢子虫是真核生物，但其核糖体及核糖体RNA为原核生物型。为探讨9种家蚕病原性微孢子虫的种属地位及亲缘关系，对已广泛用於生物进化分类的核糖体小亚单位RNA基因进行了研究。

Objective To study the significance of mutations of HBV preS and S genes in peripheral blood mononuclear cells.

目的 研究外周血单个核细胞中乙型肝炎病毒包膜抗原基因变异的意义。

Results Of a total of 100 cases, 51(51%) were confirmed as linear scleroderma, 26 (26%) as plaque morphea, 26 (26%) as deep morphea, 12(12%) as generalized morphea, and 15(15%) as a mixed subtype. Nine patients (9%) had family histories of rheumatic or autoimmune diseases, while 16(16%) might be triggered by unknown factors. Totally 84 patients underwent antinuclear antibody tests and 38 patients (45.2%) had positive results.

结果 100例患者中，51例(51%)出现线状硬皮病表现，26例(26%)出现斑块状硬斑病表现，26例(26%)出现深在性硬斑病表现，12例(12%)出现泛发性硬斑病表现，其中15例(15%)为两型混合；9例(9%)患者有风湿性或免疫性疾病家族史，16例(16%)患者可能与某种诱发因素有关。84例行抗核抗体检测的患者中，有38例(45.2%)为阳性。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。