核型

Light microscope and transmission electron microscopy showed that SMMC-7721 cells induced by SAHA had undergone the restorational alteration in morphology and ultrastructure, which were different from those of nontreated cells but were similar to those of normal cells, and the changes were as follows: the cells turned to be flat and spread; the nucleo-cytoplasmic ratio lessened and nuclear shape became rather regular; the number of nucleolus reduced and its volume lessened; euchromatin increased while heterochromatin decreased in nucleus; in the cytoplasm, mitochondria grew in number with relatively consistent structure and well-developed mitochondria cristae; Golgi complex turned to be well-developed and typical; rough endoplasmic reticulum increased. Immunocytochemistry assay showed that the expression of AFP and PCNA were declined significantly. FCM analysis showed SAHA could arrest SMMC-7721 cells in G0/G1 phase, with an accumulation of the cells in G0/G1 phase while a decrease of cells in S phase. Semi-quantitative RT-PCR detection revealed that the expression of p21WAFl mRNA was upregulated remarkably in the cells treated with SAHA.

结果：倒置显微镜和透射电镜观察显示，经SAHA处理的细胞增殖速度显著减慢，细胞体积增大，细胞核较小，形状较为规则，核仁数量减少、体积变小，核内常染色质增多而异染色质减少，核质比例减小，细胞质内线粒体数量增多、线粒体嵴发达，高尔基体较为典型，粗糙型内质网增多，呈现出与正常上皮细胞相似的形态变化；MTT比色法测定结果显示不同浓度(2.5、5.0、7.5、10.0uM)SAHA对SMMC-7721细胞的增殖均有抑制作用，并有明显的剂量依赖和时间依赖关系；免疫细胞化学检测显示SAHA能显著降低PCNA和AFP在SMMC-7721细胞中的表达；流式细胞仪检测结果显示，SMMC-7721细胞经SAHA处理后，G0/G1期细胞明显增加，S期细胞则明显减少，细胞被阻滞于G0/G1期；RT-PCR检测结果表明，SAHA作用12h后SMMC-7721细胞中p21WAF1 mRNA的表达即有增加，24h后更为明显。

Antoni type A areas consist of compact spindle-shaped cells with twisted nuclei and indistinct cytoplasmic borders. They are arranged in interlacing fascicles with nuclear palisading, whirling, and Verocay bodies.

Antoni A型区的细胞呈梭形，胞核扭曲，胞质边界模糊，细胞排列为呈束状交错或旋涡状，胞核呈栅栏状，有Verocay小体。

Studies on the regulation of malignant phenotype expression in a mouse plasmocytoma cell line (SP2/0) by means of cellular engineering techniques of cell hybridization between the fusion of the mouse myeloma cells with rat intermediate or late erythroblasts.

三、利用细胞杂交的细胞工程手段，首次系统地研究了大鼠中幼、晚幼成红细胞与小鼠浆细胞瘤（SP2/0）细胞融合后恶性表型的表达状态，分析自然去核前红细胞胞质对骨髓瘤细胞恶性增殖的调控作用，以及晚幼期固缩核重新被激活的可能性。

to use spinal lateral approach under the guidance of the c-arm x-ray machine, the needle position will be part of the disc, after successful percutaneous insert the plasma sword to melt herniated nucleus pulpous with the power, and then enter the moderate ozone to further expand the scope of ablation, and strengthen the inter-disk degradation and inhibition of the release of inflammatory mediators to eliminate protrusion of nerve roots caused by the oppression and inflammatory stimulation.

采用脊柱后外侧入路，在c型臂x线机引导下，将穿刺针定位于椎间盘部位，经皮穿刺成功后先插入等离子刀，通电后消融髓核使髓核皱缩，再打入适量臭氧，进一步扩大消融范围，加强降解间盘和抑制炎性介质的释放，来消除因间盘突出引起的对神经根的压迫和炎性刺激。

Organic matrixs coming from cells play role in nucleation, growth, morphology, polymorphology of inorganic materials. This control of organic matrixs are mainly behaved by the interfaction between organic matrix and inorganic materials .The processing of organic matrix controlling inorganic material is called as molecular recognition. The mechanism of organic-inorganic molecular recognition contains three facts such as electrostatic attraction, lattice geometry matching and stereochemical complimentary.

细胞分泌的有机基质在无机矿物的成核，生长，形貌，多型和结晶学定向等过程中起到了调控作用，基质对矿化的这种调控作用具体表现在有机或生物大分子和无机离子在界面上的相互作用，有机质对无机晶体的成核，生长，晶形及结晶学定向等的控制过程称为分子识别，有机-无机界面分子识别机制主要包括静电作用，晶格几何匹配和立体化学互补三个方面。

Through cyclic hydrogen bonding with motif formed by coordinated water molecules and coordinated hydroxyl groups of dhbd from adjacent tetranuclear units, these adjacent neighbors are connected to afford a 1D supramolecular chain along axis a, which is further extended via aromatic intercalations and other crystallization water molecules into a 3D structure with channels.

毗邻的四核单元通过配位H2O分子和配位羟基O原子形成的 R 22(8)型环形氢键桥的连接，沿a轴方向构建了四核单元交替相连的一维超分子链；链间籍苯环的π-π堆积作用和晶格水分子氢键链的连接进一步扩展为具有隧道的三维结构。

The mitotic count was higher than 5/50HPF in 5 cases in which the diameters of tumor were above or equal to 5 cm. Cystification, hemorrage or necrosis were found in 4 cases and mucification in 1 case. The mitotic count was 2-5/50HPF in 2 cases which were epithelioid cell and tumor diameter were 2-4 cm with mucification in 1 case.

核分裂像大于5/50HPF的5例中肿瘤直径均大于或等于5 cm，其中4例伴有囊性变、出血或坏死，1例伴有黏液样变。2例核分裂像在2~5/50HPF者均为上皮样细胞型，其中1例伴有黏液样变，肿瘤直径均2~4 cm。

In OWE-SOJ test, a fluffy colony without clampconnection formed in the A=B≠mating interaction where nuclear migration occurs, whilea barrage colony without clamp connection formed in A≠B= interaction where nuclearmigration do not occur. The two colonies could be distinguished clearly from each other.

而在OWE-SOJ试验中，发生核迁移的A=B≠交配反应形成无锁状联合的绒毛状菌落，而不发生核迁移的A≠B=反应形成无锁状联合的栅栏型菌落，两种菌落可以清楚地区别开来。

In OWE-SOJ test, a fluffy colony without clamp connection formed in the A=B≠ mating interaction where nuclear migration occurs, while a barrage colony without clamp connection formed in A≠B= interaction where nuclear migration do not occur. The two colonies could be distinguished clearly each other.

而在OWE-SOJ试验中，发生核迁移的A＝B≠交配反应形成无锁状联合的绒毛状菌落，而不发生核迁移的A≠B＝反应形成无锁状联合的栅栏型菌落，两种菌落可以清楚地区别开来。

The thymidine kinas gene was cloned from human herpes simplex virus Ⅱ by PCR techniques; eukaryon expressing vector pcDNA3/tk and pcDNA3/angio/tk fusion gene was constructed by DNA recombination; The treating effects of human primary liver cancer strain SMMC-7721 in vitro and nude mouse model transplanted with human liver cancer in vivo are studied.

通过PCR技术，由人单纯疱疹病毒DNA克隆获得单纯疱疹病毒Ⅱ型胸苷激酶基因；通过基因工程技术，构建真核表达质粒pcDNA3/tk及融合基因真核表达质粒pcDNA3/angio/tk；研究其对人原发性肝癌SMMC-7721细胞株的体外杀伤作用及对人原发性肝癌裸小鼠移植瘤模型的治疗作用。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。