核型

Most proerythroblast showed of irregular nuclei, while the Swisscheeseappearance of the heterochromatin was usually found in basophilic and polychromatic erythroblast.

CDAⅠ型的主要超微结构特点为幼红细胞巨幼样增生，其次是中幼阶段核膜损伤和晚幼阶段的核溶解和核碎裂，生物膜系统广泛破坏是CDAⅠ型主病理机制。

The dominant male sterility was expressed normally in the new Taigu genic sterile wheat carrying the intergenomically translocated Ms2, and the female fertility mechanism in its male sterile plants was normal as well. Observation of the chromosome configuration at meiosis of pollen mother cells of the young ears of the sterile plants showed that they were euploid plants (2n=42). No configurations different from those of the Taigu geneic sterility gene located at the original locus were noticed of the Ms2 intergenomically translocated back into the common wheat.

所获携带易组Ms2基因的新型太谷核不育小麦其显性雄性不育特性表达正常，且雄性不育株的雌性可育机制正常，对不育株幼穗花粉母细胞减数分裂期染色体构型的观察可见其为整倍体(2n=42)，尚未发现回归普通小麦的易组太谷核不育基因与原位点的太谷核不育基因有不同的表型。

Results Preoperative MRI classification indicated: intact annuals in 82 levels, annuals tear with intact PLL in 123 levels, PLL tear in 70 level, free protruded mass under PLL in 48 levels; on the other hands, the amount of annuals tear and free protruded mass under PLL introoperative observation were more than that preoperative MRI indicated: intact annuals in 38 levels, annuals tear with intact PLL in 165 levels, PLL tear in 62 level, free protruded mass under PLL in 63 levels; dural sac penetration in 2 levels.

结果： MRI术前分型：纤维环完整者81个椎间隙、纤维环破裂后纵韧带完整者123个椎间隙、后纵韧带破裂者70个椎间隙、髓核后纵韧带后方游离者48个椎间隙。手术发现纤维环破裂数量以及后纵韧带后方游离髓核片数量明显高于MRI的提示：纤维环完整型38个椎间隙、纤维环破裂后纵韧带完整165个椎间隙、后纵韧带破裂髓核疝62个椎间隙、后纵韧带后方游离髓核63个椎间隙、硬膜囊下疝2个椎间隙。

There were on unique different protein observed in flag leaf at different development stage; but unique different protein were found in young spike at uninucleate microspore stage and non-1B/ 1R type male sterile lines lack a 17kD polypeptide compared with maintainers; at binucleate microspore stage and non-1B/ 1R type male sterile lines increase 17kD polypeptide compared with maintainers.

利用SDS-PAGE凝胶电泳技术对采用丙酮沉淀法提取的K型，C49S型和YS型小麦不育系和保持系旗叶小穗发育的单核期和二核期中的可溶性蛋白进行比较后发现，非1B/1R类K型不育系732A在小穗发育的单核期比同时期相应保持系732B少1条分子量为17kD的多肽，在小穗发育的二核期比同时期相应保持系732B多1条分子量为17kD的多肽。

Narrow Line Seyfert 1s (NLS1s, including high luminosity quasars with similar properties) are very important extrema of type Ⅰ AGNs. The unusualness of NLS1s is very useful to test the viabilities of AGNs models and thus provide a new way probing into AGN phenomena. The ratio of Fe to α-element (e. g., O, Ne, Mg...), Fe/α, can serve as an excellent indicator for constraining the ages of star-forming systems.

中文题名窄线SeyfertⅠ星系与活动星系核光学FeⅡ发射线副题名外文题名论文作者周宏岩导师周又元王挺贵学科专业天体物理研究领域\研究方向学位级别博士学位授予单位中国科学技术大学学位授予日期2003 论文页码总数127页关键词活动星系核河外星系馆藏号BSLW /2003 /P15 /7 窄线SeyfertⅠ型星系(Narrow Line Seyfert 1，NLS1，包括具有类似性质的类星体)是具有极端性质非常重要的一类Ⅰ型AGN，对NLS1的深入研究是限制和完善活动星系核理论模型的一条新途径。

The acetated and hydrogenated urushiol dimer has been separated,and studied by hetero-J resolved 2D-NMR,homo- and heteronuclearchemical shift correlation 2D-NMR and long-range coupled chemicalshift correlation 2D-NMR.The results show that the urushiol dimerare most diphenyl and alkyl phenyl ether structures.Furthermore,IR and MS data also has conformed this structures.

本文将酶催化预聚后的生漆，催化加氢后，乙酰酯化，再进行离心旋转薄层层析，分离得到二聚体主要组分，用异核J分辨谱，同核化学位移相关，异核化学位移相关，远程偶合异核化学位移相关等2D—NMR技术，全面研究了其结构，得到了联苯型和烷基芳醚型聚合结构的结论。

To study the similarities and differences between male sterility induced by physiology and heredity, different male-sterile materials induced by heredity [S-type male-sterile line ms2611, ms1376] and physiology (induced by high temperature and chemical hybridizing agents SQ-1), Xinong 1376 and Xinong 2611 were used as materials, and their spikes at mononucleated prophase stage, anther and flag leaf at mononucleated anaphase stage, binucleated stage and trinucleated stage were used to conduct protein analysis.

为了研究小麦遗传型和生理型雄性不育在生化机制上的异同，以遗传型［S型不育系ms2611和ms1376］和生理型雄性不育小麦西农2611和西农1376（高温诱导和化学杀雄剂SQ-1诱导的雄性不育）为材料，分别取其小孢子处于单核早期的幼穗，单核后期、二核期、三核期的花药及旗叶进行了可溶性蛋白质的比较研究。

The male sterility resulted from nucleus-cytoplasmic interaction (nuclear genome of common wheat--cytoplasmic genome of D〓-type cytoplasm donor), D〓-type CMS was sporophytic sterility pattern.

一、D〓型CMS系的花粉育性对光周期的变化不敏感（不同于已有光敏不育性的报道），属非光敏细胞质雄性不育系；其不育性起因于核质互作（普通小麦核基因组--D〓型细胞质变异基因组）、属孢子体不育类型；雄性不育受核内隐性基因控制、育性恢复受核内显性基因控制。

By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length PI, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length PI, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3.1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus, which were expressed in BHK-21 cells, were confirmed by sandwich-ELlSA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P 12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3.1/lFN which includes the gene IFN-a of cattle. Subsequently, Recombinant plasmids were injected to catties with or without pcDNA3.1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies liters were analyzed by micro-neutralization assay.

为研制带有O型口蹄疫病毒(Foot-and-Mouth Disease Virus，FMDV)China99株结构蛋白基因及多个非结构蛋白基因的DNA疫曲，本研究通过定点突变方法和PCR扩增方法，获得包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C以及部分2B、3B编码基因的片段P12X3C和包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C、3D以及部分2B、3B编码基因的片段P12X3C3D，将获得的基因片段直接／酶切后与同样处理的真核表达质粒连接，分别得到重组质粒pcDNA3.1／P12X3C和pcDNA3.1／P12X3C3D、pTARGET／P12X3C3D；对重组质粒进行序列测定、分析，并将重组质粒分别转染BHK-21细胞，通过双抗体夹心ELISA方法和间接免疫荧光标记方法检测细胞中FMDV抗原的表达，用电子显微镜观察病毒空衣壳的组装；为评价重组质粒作为DNA疫苗对实验动物及本动物的免疫效果，将重组质粒经肌肉注射方法接种豚鼠，并与酵母表达的纯化FMDV China99株3D蛋白及带有牛α干扰素的真核表达质粒pcDNA3.1／IFN分别／同时免疫，第二次免疫后第三周豚鼠攻以100ID_(50)或1000ID_(50)的O型FMDV China99株：随后将质粒pcDNA3.1／P12X3C、pcDNA3.1／P12X3C3D与带有牛α干扰素的真核表达质粒pcDNA3.1／IFN同时免疫牛，三周后经牛舌皮攻以10~4ID_(50)的O型FMDV China99株。

The genes of hammerhead ribozymes targeting against two and three sites on negative strand of potato spindle tuber viroid were designed, synthesized and cloned according to the action manner of hammerhead ribozyme.

根据锤头型核酶的作用模式，设计、合成并克隆了特异切割马铃薯纺锤形块茎类病毒负链RNA不同区域位点的双价和三价锤头型核酶基因。通过体外转录，将PSTVd负链RNA分别与双价和三价核酶混合，37℃温育 2h。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。