核型

The experimental results indicate that when introducing XT-β nucleator into PP, the size of spherulite of the specimen obviously reduces and there appear typical characteristic diffraction peaks of β crystal form in the wide-angle X-ray diffraction pattern....

结果表明：在PP中引入XT-β晶型成核剂后，试样的球晶尺寸明显减小，广角X射线衍射谱图出现了典型的β晶型特征衍射峰；试样的结晶速率、结晶温度明显提高，差示扫描量热法谱图出现α晶型、β晶型2个熔融峰，β晶型含量达到总结晶量的50%～80%；XT-β晶型成核剂可使体系保持较好的力学性能，PP负荷下热变形温度提高15～40℃。

As to Balilla erect panicle type,(the same subject for the following 11-16), the curvature of panicle neck had a very significantly positive correlation with panicle length, neck panicle length, neck panicle internode length, the secondary internode length, leaf length and leaf sheath length, and had an opposite correlation with leaf width and density of seed setting.

来自吉林的直立穗型品系Z1和来自黑龙江的直立穗型品种龙交91060—2与巴利拉型直立穗型品种的直立穗其因分属于3个不同的位点，各自位于3对同源染色体上，彼此遵循基因的自由重组和独立分配规律。Z1由1对显性主效核基因控制，龙交91061—2由1对隐性主效核基因控制。3个直立穗型基因位点可能具有累加效应，直立穗型效应位点越多，则颈穗弯曲度越小，穗的直立性越强。

The cancer cells are in different differentiation periods: the chromatin of the young cancer cell's nucleus is rich in color and its cytoplasm is basophilous. The young cancer cells don't form into typical glandular cavity; The mature cancer cell is columnar or cubical and its nucleus is located at the base of the cell in gland tube-like arrangement; The decrepit cancer cell stain thin while its nucleus stain dense. The severer's nucleus disintegrate into small fragments. The decrepit cancer cell's arrangement is disorganized, only keeping its glandular shape.

显微镜下癌细胞呈现不同的分化程度：幼稚型癌细胞胞核染色质丰富，胞质嗜碱性，不形成典型腺腔；成熟型癌细胞呈柱状或立方状，细胞核位于细胞基部，呈腺管样排列；衰老型癌细胞胞桨染色变淡，胞核浓染，严重者胞核碎裂成细小碎片，衰老型癌细胞排列紊乱，仅保留腺体样结构轮廓。

However, grade II CT signs did not always result from type 2 occlusion; 21.1% of patients with grade II CT signs had type 4 occlusion. When the MCA trunk is short, the lateral group of the lenticulostriate arteries may arise from the M2 segment[2, 12]. In such cases, type 4 occlusion may also involve the lateral or posterior part of the putamen.

但是，II级早期CT征象（部分壳核后外侧区出现低密度）并非仅仅由2型大脑中动脉闭塞（包括部分豆纹动脉在内的大脑中动脉主干闭塞，豆纹动脉部分受累）引起；本研究中，21.1%深部大脑中动脉分布区出现II级早期CT征象（部分壳核后外侧区出现低密度）的患者，是因4型大脑中动脉闭塞（大脑中动脉M2段闭塞）引起的；这些患者的大脑中动脉主干均很短，因此外侧豆纹动脉群（lateral lenticulostriate arteries）是由大脑中动脉M2段发出的；此时，4型大脑中动脉闭塞（大脑中动脉M2段闭塞）也可以导致壳核后部或外侧部出现低密度（深部大脑中动脉分布区II级早期CT征象）。

These 375 patients had a median age of 50.57±10.46(range,19-72) with 87.4%HBsAg positive and 4.3%anti-HCV antibody positive;The apparent peak incidence age was 40～60 years old,and the ratio of male to female was 10.7:1;The 3 and 5 year postoperational survival rate were 52%and 38%;The tumour numbers(p=0.000),tumor size(p=0.025),histological pattern (p=0.000),nuclear features(p=0.000),differentiation(p=0.001) and vascular invasion(p=0.000) were significantly correlated with prognosis.The postoperational survival time of thin trabeculae pattern,compact pattern and pseudoglandular pattern were significantly longer than that of thick trabeculae, scirrhous pattern,and solid patternp＜0.009the postoperational survival time of 1 and 2 grade based on nuclear features were significantly longer than that of 3 and 4 grades(p=0.000The small cell variant,osteoclast-like giant cell variant, and spindle cell variant were mainly composed of thick trabeculae pattern and solid pattern,which were significantly different from that of clear cell variant.

结果1。本组资料显示肝细胞癌发病年龄19～72岁，平均50.57±10.46岁，发病高峰年龄40～60岁，男女比例为10.7：1；HBsAg87.4%，anti-HCV抗体4.3%；术后3年生存率为52%，5年生存率为38%；肿瘤数目(p=0.000)、肿瘤大小(p=0.025)、组织学结构类型(p=0.000)、核分级(p=0.000)、分化程度(p=0.001)及血管浸润均(p=0.000)与预后明显相关；其中组织学结构类型中细梁状型生存时间与致密梁状型和腺样型无明显区别(p＞0.05)而明显高于粗梁状型、实性型和硬化型(p≤0.009)，硬化型生存时间与实性型之间无明显区(p＞0.05)而明显低于其余各型p≤0.006核分级1级与2级生存时间无明显区别(p＞0.05，核分级3级与4级生存时间无明显区别(p＞0.05)，而核分级3级生存时间明显低于2p=0.000小细胞型、巨细胞型和梭形细胞型主要由实性型和粗梁状型组织学结构类型构成，明显不同于透明细胞型(主要由细梁状型和粗梁状型构成(p≤1.006)。2。

Both were measured using X-ray single crystal diffraction. For the crystal in MeOH, X-ray in compound. In an ansymmetry unit, there are two Cu atoms, two ligands and twosolvent methanol molecules. The tetranuclear complex contains two kinds of Cucenter.

本文在MeOH中得到了阶梯型四核铜骨架配合物；在THF中得到了扭曲的立方烷型四核铜骨架配合物，经晶体结构分析表明，阶梯型四核铜骨架配合物是由两个Cu2被μ~3-剪刀撑二个醇羟基O所连接形成中心对称的二铜二聚体结构。

By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length P1, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length P1, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3. 1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus , which were expressed in BHK-21 cells, were confirmed by sandwich-ELISA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3. 1/IFN which includes the gene IFN-α of cattle. Subsequently, Recombinant plasmids were injected to cattles with or without pcDNA3. 1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies titers were analyzed by micro-neutralization assay.

为研制带有O型口蹄疫病毒(Foot-and-Mouth Disease Virus，FMDV)China99株结构蛋白基因及多个非结构蛋白基因的DNA疫苗，本研究通过定点突变方法和PCR扩增方法，获得包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C以及部分2B、3B编码基因的片段P12X3C和包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C、3D以及部分2B、3B编码基因的片段P12X3C3D，将获得的基因片段直接/酶切后与同样处理的真核表达质粒连接，分别得到重组质粒pcDNA3.1/P12X3C和pcDNA3.1/P12X3C3D、pTARGET/P12X3C3D；对重组质粒进行序列测定、分析，并将重组质粒分别转染BHK-21细胞，通过双抗体夹心ELISA方法和间接免疫荧光标记方法检测细胞中FMDV抗原的表达，用电子显微镜观察病毒空衣壳的组装；为评价重组质粒作为DNA疫苗对实验动物及本动物的免疫效果，将重组质粒经肌肉注射方法接种豚鼠，并与酵母表达的纯化FMDV China99株3D蛋白及带有牛α干扰素的真核表达质粒pcDNA3.1/IFN分别/同时免疫，第二次免疫后第三周豚鼠攻以1OOID〓或1000ID〓的O型FMDV China99株；随后将质粒pcDNA3.1/P12X3C、pcDNA3.1/P12X3C3D与带有牛α干扰素的真核表达质粒pcDNA3.1/IFN同时免疫牛，三周后经牛舌皮攻以10〓ID〓的O型FMDV China99株。

Automatic classification of chromosomes could be made to save both manpower and time.

染色体的自动核型分类可以为完成这一任务节省人力和时间。

Result: A total ratio of hybridization was 73.53%; in triploid zygotes after IVF, the ratios of triploid, diploid and monoploid were 92.31%, 3.85% and 3.85%, respectively.

结果：分析有杂交信号的3PN卵子68个，IVF后3PN的三倍体率为92.31%，二倍体率为3.85%，单倍体率为3.85%；共存在3种三倍体核型：XXX、XXY、XYY。

Karyotype analysis method is a basic technique in cytogenetics,cytotaxonomy and other related subjects.

核型分析是细胞遗传学、染色体工程、基因定位及细胞分类学等学科的基本研究方法[1]。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。