核型

Bombyx Morinuclear Polyhedrosis Virusis a kind of circular DNA virus.

家蚕核型多角体病毒是一类环状DNA病毒。

Two bio-insecticides, Bacillus thuringiensis and Ectropis oblqua Nuclear Polyhedrosis Virus, are commonly used for control of pests as mixtures.

苏云金杆菌和茶尺蠖核型多角体病毒等，两类生物源杀虫剂常以复配混用方式应用。

Five fetuses were diagnosed with female pseudohermaphroditism (21-hydroxylase deficiency in 3 and urorectal septum malformation sequence in 2). Four fetuses were diagnosed with male pseudohermaphroditism (1 with steroid sulfatase deficiency, 1 with presumed camptomelic dysplasia, and 2 undetermined).

在这16个胎儿中有5个被诊断为女性假两性畸形（其中3个有21-羟化酶分泌不足，2个有尿道直肠隔畸形），4个被诊断为男性假两性畸形（其中1个有类固醇硫酸酯酶分泌不足，1个推测可能为弯肢性发育异常，另外2个原因未定），5个有染色体异常，2个有性逆转，核型为（46，XX+SRY）。

Clinical featu res: 18-year-old female, 155 cm high, 45 kg weight, uterus: 22×18 ×17 mm, unclear bilateral ovaries, normal pudendum, breast

A： 中期G带图； B：异常染色体核型分析图； C：家系图； D：减数分裂四分体配对模式图。临床表现：女， 18岁，身高155 cm，体重45 kg，子宫大小22

We also analysed the SCs of 30 spermatocytes at pachytenes, showing 20 autosomal bivalents, a quadrivalent 11; 18 and a sexual bivalent.

病例4为受孕浪费、生育过一例具有21三体特征患儿的患者，体细胞染色体分析表明，其核型为45，XY，-21，-21，+rob（21q；21q）。

The synthesized cDNA is a continuous band less than 6. 0kb shown on x-film through α〓PdCTP which was inserted in the first strain of cDNA. The titer of the Ha-cDNA library was about 1. 5×10〓cfu/ml which could represent all protein-encoding sequences from Hz-AM1 cells; Namely, the cDNA library has representativity. After the successful construction of the Ha-cDNA library, the open-reading frame of vp39 gene of Heliothis armigera was then fused in frame with the Ga14-DNA-binding domain in the pGBT9 plasmid and the fusion protein Ga14-VP39 was expressed in yeast to be used as bait protein.

在成功构建棉铃虫细胞Hz-AM1cDNA文库的基础上，本文接着将棉铃虫核型多角体病毒衣壳蛋白VP39（HaNPV-VP39）基因克隆到酵母双杂交系统(yeasttwo-hybrid system)中的质粒pGBT9上。pGBT9上含有酵母菌转录激活因子GAL4 DNA结合域的编码区（GAL4-DBD），使克隆到质粒pGBT9上的HaNPV-vp39基因与GAL4-DBD基因融合，在酵母菌中表达DBD-vp39融合蛋白，该融合蛋白即为酵母双杂交系统中的诱饵蛋白。

Cell lines was established in vitro cell culture system. Only one of it grows well during gene targeting operation. The sex of these cell lines was identified by Sry-PCR.Using lipotransfection method to transfer pZJ into pig fetal fibroblast cell. After 7 days G418 selection, 30 neo-positive cell clones were obtained, among them, 12 of which is non-green ones.

用Sry-PCR法和染色体核型分析法鉴定细胞系的性别，采用优化的脂质体转染法将pZJ转染猪胎儿成纤维细胞，经7天G418(600μg／ml)药物筛选，共获得30个neo阳性细胞克隆，其中12个为非绿色细胞克隆，仅有7个可以扩大培养。

There were different views about which chromosome is the sex chromosome.

对不同核型中性染色体的确定曾有不同的观点。

And the research materials were often meiotic pachytene chromosomes of sex gland and mitotic prometaphase of early embryos and diapause eggs\' somatic cells.

早期，家蚕染色体识别和基因定位研究主要利用性腺减数分裂粗线期的染色体、早期胚胎和滞育卵有丝分裂早中期的染色体作为材料，按照相对长度和部分限性易位系统或缺失等来进行核型分析。

Results Stable Spectral Karytyping method was set up and extraordinary multiplicity of structure, including metathesis、depletion、amplification、rearrangement、equiarm、marker-body and so on were found by this methad.

结果 我们建立了稳定的光谱核型分析方法，发现肿瘤实体瘤染色体异常呈多倍体且结构异常复杂，包括易位、缺失、扩增、重排、双着丝粒、等臂及标记染色体等。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。