核嘌呤

Synthesis of target compounds namely: to vanillic acid as the starting material with methanol under reflux conditions for 4 - hydroxy -3 - p-methyl, then ether, and nitration, reduction, cyclization reaction 6 - methoxy -7 - benzyloxy-quinazoline -4 - one, and then by the chloride in place of aniline, benzyloxy-off, such as etherification reaction of the target compounds; target compounds with the second and third occurrence of substitution reactions of amines by the TM1, that is, 4 - amino-benzene -6 - methoxy -7 - [2 - hydroxy -3 -(N, N-diethyl amino) oxy c] quinazoline; with ether occurred Ornidazole reaction of TM2, namely, 4 - amino-benzene -6 - methoxy -7 - [2 - hydroxy -3 -(2 - methyl -5 - nitroimidazole) C oxy] quinazoline.

本论文以嘌呤类似物喹唑啉为母核，分别在其4位和7位引入结构多样的取代苯氨基和柔性侧链，设计了一系列4-取代苯胺基-6-甲氧基-7-(2-羟基取代丙氧基)喹唑啉类化合物。目标化合物的合成即：以香草酸为起始原料，与甲醇回流条件下得到4-羟基-3-甲氧基苯甲酸甲酯，然后经过醚化、硝化、还原、环合反应得到6-甲氧基-7-苄氧基喹唑啉-4-酮，然后再经氯化、取代苯胺、脱苄氧基、醚化等反应得到目标化合物；目标化合物与二乙胺发生胺取代反应得到了TM1，即4-苯氨基-6-甲氧基-7-[2-羟基-3-丙氧基]喹唑啉；通过与奥硝唑发生醚化反应得到TM2，即4-苯氨基-6-甲氧基-7-[2-羟基-3-(2-甲基-5-硝基咪唑)丙氧基]喹唑啉。

After thecomplete genome extraction of the strain was performed, the genomic DNA was partiallydigested by restriction enzyme Sau3AⅠ, the DNA fragments from 1 to 5Kb was clonedinto prokaryote expression vector pET-28a-c, and transformed host bacteria. The resultsshowed that we succeeded in constructing the gene expression library of haemophilusparasuis serovar 5, which is fundamental for the study of advanced gene screening. Inaddition, primer design was performed based on haemophilus influenzae in this study. In addition, PCR was performed by using genomic DNA of haemophilus parasuisserovar 5 as the template. The results demonstrated that we obtained two neo-gene:23SrRNA gene(conserved gene belonging to the large-subunit of ribosome) and adenylatecyclase gene(encodes adenylate cyclase and participates in converting adenyl nucleosidetriphosphate to cyclic adenosine3",5"-monophosphate). Furthermore, the phylogeneticanalyses between the species was performed, and neighbor-joining tree was constructedbased on comparison of 23S rRNA gene sequences, so it was illuminated betweenHaemophilus parasuis and other species in molecular evolution relationship.

选择我国流行优势菌株副猪嗜血杆菌血清5型地方株为研究对象，提取细菌基因组DNA，用限制性内切酶Sau3AⅠ对基因组DNA进行部分酶切，回收大小为1～5Kb的DNA片段，将其连接入原核表达载体pET-28a-c，最后转化宿主菌，结果成功地构建了基因表达文库，为后续的基因筛选工作奠定基础；另外，本研究选择嗜血杆菌属的流感嗜血杆菌为参考对象进行引物的设计，以副猪嗜血杆菌血清5型地方菌株的基因组DNA为模板，进行PCR扩增反应，结果表明成功地获得两个新基因：23S rRNA基因(存在于核糖体大亚基中的保守性基因)和腺苷酸环化酶基因(负责将腺嘌呤核苷三磷酸转变为环腺苷酸)，并进一步做了不同物种之间的分子系统发育分析，构建了基于23S rRNA基因的邻接法系统发育树，阐明了副猪嗜血杆菌与其它菌种的分子进化关系。

Because adenine methylation does not occur endogenously in most eukaryotes, it provides a unique tag to mark protein interaction sites.

由于腺嘌呤甲基化不在大部分的真核细胞中内源性发生，它提供了一个单一的tag来标记蛋白质相互作用位点。

These compounds were identified as follows:chrysophanol (FP-1),physcion(FP-2),eriosematin(FP-3),scoparone(FP-4),lupeol(FP-5),betulinic acid(FP-6),3\',4\'-Dihydroxy-trans-cinamic acid octacosyl ester(FP-7),β-sitosterol (FP-8),flemiphilippinone A(FP-9),monopalmitin(FP-10),emodin(FP-11),islandicin (FP-12),salicylic acid(FP-13),p-methoxyphenylpropionic acid(FP-14),trideca-1, 3-diene(FP-15),lupinifolin(FP-16),flemichin D(FP-17),flemiphilippinin A(FP-18), auriculasin(FP-19),erythrinin B(FP-20),6-C-prenylluteolin(FP-21), 8-(1,1-dimethylallyl) genistein(FP-22),flemiphilippinin E(FP-23),flemiphilippinin F (FP-24),5,7,3\',4\'-tetrahydroxy-6,8-diprenylisoflavone(FP-25),flemiphilippinin D (FP-26),dorsmaninsⅠ(FP-27),osajin(FP-28),6,8-diprenyleriodictyol(FP-29), lupinalbin A(FP-30),genistein(FP-31),3\'-O-methylorobol(FP-32),orobol(FP-33), 5,7,2\',3\',4\'-pentahrdroxyflavone(FP-34),the mixture of biochanin A and prunetin (FP-35 and 36),genistin(FP-37),sophororicoside(FP-38),3\'-O-methylorobol-7-glucoside(FP-39),the mixture of sissotrin and prunetin 4\'-O-β-D-glucoside(FP-40 and 41),adenosine(FP-42) and luteoloside(FP-43,mixture).

这些化合物分别为大黄酚(FP-1)、大黄素甲醚(FP-2)、eriosematin(FP-3)、滨蒿内酯(FP-4)、羽扇豆醇(FP-5)、白桦酸(FP-6)、咖啡酸二十八烷酯(FP-7)、β-谷甾醇(FP-8)、蔓性千斤拔酮A(FP-9)、单棕榈酸甘油酯(FP-10)、大黄素(FP-11)、islandicin(FP-12)、水杨酸(FP-13)、对甲氧基苯丙酸(FP-14)、十三烷-1，4-二烯烃(FP-15)、lupinifolin(FP-16)、千斤拔素D(FP-17)、蔓性千斤拔素A(FP-18)、auriculasin(FP-19)、erythrinin B(FP-20)、6-C-异戊烯基木犀草素(FP-21)、8-(1，1-二甲烯丙基)-染料木黄酮(FP-22)、蔓性千斤拔素E(FP-23)、蔓性千斤拔素F(FP-24)、5，7，3′，4′-四羟基-6，8-双异戊烯基异黄酮(FP-25)、蔓性千斤拔素D(FP-26)、dorsmaninsⅠ(FP-27)、osajin(FP-28)、6，8-双异戊烯基圣草素(FP-29)、lupinalbin A(FP-30)、染料木黄酮(FP-31)、3\'-O-甲基香豌豆苷元(FP-32)、奥洛醇(FP-33)、5，7，2′，3′，4′-五羟基黄酮(FP-34)、鹰嘴豆素甲和樱黄素的混合物(FP-35和FP-36)、染料木苷(FP-37)、槐属苷(FP-38)、7-葡萄糖基-3\'-O-甲基香豌豆苷(FP-39)、印度黄檀苷和樱黄素4′-O-β-D-葡萄糖苷的混合物(FP-40和FP-41)、腺嘌呤核苷(FP-42)和木犀草苷(FP-43，混合物)。

The DFT (B3LYP/6-31＋G**) method was used to study the conformations and isomerization of the isolated and monohydrated 2',3'-dideoxy-2',3'-didehydroguanosine (D4G) molecules in gas phase.

采用密度泛函方法在B3LYP/6-31＋G**水平上研究了2'，3'-二脱氧-2'，3'-二去氢鸟嘌呤核苷分子(D4G)的构象。

The DFT B3LYP/6-31+G(superscript ** method was used to study the conformations and isomerization of the isolated and monohydrated 2', 3'-dideoxy-2', 3'-didehydroguanosine (D4G) molecules in gas phase.

采用密度泛函方法在B3LYP/6-31+G水平上研究了2'，3'-二脱氧－2'，3'-二去氢鸟嘌呤核苷分子(D4G)的构象。

The binding behavior and kinetics of uncoupling protein with its ligand, the Fourier-transformation infrared spectra of uncoupling protein and its changes during ligand-binding process, the inhibition of Guanosine 5＇-Tetraphosphate (G-4-P) and Adenine-β-Darabinofuranoside 5＇-triphosphate on the binding of uncoupling protein with GTP were studied.

本文对解偶联蛋白与配基的结合及其结合动力学，解偶联蛋白及其与配基结合过程中的傅里叶变换红外光谱及其光谱变化，鸟嘌呤核苷四磷酸（G-4-P）和9-β-D-阿拉伯糖基呋喃5＇-三磷酸对解偶联蛋白与GTP结合的影响进行了研究。

A form of post-transcriptional modification to mRNA in eukaryotes in which adenine is deaminated to form inosine, an unusual base.

真核生物中对mRNA进行转录后修饰的一种形式，修饰时将腺嘌呤脱氨基形成次黄苷，次黄苷是一种非普通碱基。

A I editing A form of post-transcriptional modification to mRNA in eukaryotes in which adenine is deaminated to form inosine, an unusual base.

A I 编辑真核生物中对 mRNA 进行转录后修饰的一种形式，修饰时将腺嘌呤脱氨基形成次黄苷，次黄苷是一种非普通碱基。

Objective: Design and synthesize the oligodeoxynucleotides containing unm- ethylated CpG dinucleotides to screen out the optimal CpG-ODN that could markedly enhance the immune function of cord blood mononuclear cells in health newborns. Moreover, observe the action of CpG-1826 (5'-TCCATGACGTTC CTGACGTT-3') as the immune adjuvant of the hepatitis B vaccine on the pregnant mice and their neonatal mice.

目的：设计并合成含非甲基化胞嘧啶和鸟嘌呤二核苷酸的寡聚脱氧核糖核苷酸，以期筛选出对新生儿脐血单个核细胞免疫刺激作用较强的CpG-ODN；观察不同剂量CPG-1826(5'-TCCATGACGTTCCTGACGTT-3')辅助乙肝疫苗免疫孕鼠及仔鼠的作用。

What would he tell Judith and the children?

他将怎样告诉朱迪丝和孩子们呢？

I this is at that time, the opinion with peacockish true girl is full of in a heart.

这就是当时的我，一个心中布满虚荣的女孩子真实的想法。