核周体

Methods: Density gradient centrifugalization and absorption technique wererespectively applied to detach peripheral blood mononuclear cells andperipheral blood lymphocytes,T lymphocytes were got by Nylon postabsorption method, and CD4+T and CD8+T lymphocytes divided with anti- CD4 andanti-CD8 antibody by alexin cytotoxic method.

密度梯度离心法分离外周血单个核细胞，玻璃器皿吸附法分离外周血淋巴细胞，尼龙棉柱法分离 T 淋巴细胞，补体介导的细胞毒法分别制备 CD4+T和 CD8+T 淋巴细胞，采用 T 淋巴细胞与角质形成细胞共培养技术研究银屑病患者外周血 T 细胞对角质形成细胞的影响，免疫组化法检测共培养角质形成细胞 Ki67、c-Myc 及 Bcl-xL 蛋白的表达。

Blood samples from 23 Huacaya alpacas ,3 males and 20 females ,were used to study chromosome karyotype ,so as to provide some effective cytogenetics basis for the selectioiymprovement by crossing and disease diagnosis of alpacas ,and genetic mechanisms of sex determination. Peripheral blood lymphocyte culture was used to prepare chromosome. Methods of trypsase-EDTA and BSG (Barium hydroxide / Saline / Giemsa)were used for G-banding and C-banding respectively . The results showed as follows:Firstly , the number of diploid chromosomes was in the alpacas 2n=74,with the karyotype 74, XY and 74, XX for males and females respectively.

为了从选种、杂交改良、疾病诊断以及性别决定的遗传机制等方面为羊驼的繁育与推广提供更为有效的细胞遗传学资料，本试验采用外周血淋巴细胞培养法制备染色体，分析了23只胡阿基亚型羊驼(Huacaya alpaca，雌20只，雄3只)的染色体及其核型，并通过BSG法(Barium hydroxide／Saline／Giemsa，氢氧化钡-盐溶液-吉姆萨，也即Sumner BSG法)与胰酶-EDTA法分别对羊驼的C-分带和G-分带进行分析，结果表明：(1)羊驼二倍体染色体数目为2n=74，雄性羊驼核型为74，XY；雌性羊驼核型为74，XX。

These DSs were first observed at 9 weeks in the distal part of ascending fibers that consist of gracile fasciculus and cerebellospinal-gracile nucleus and white matter of cerebellum. They extend along medulla oblongate to medulla cervix、medulla thorax、medulla lumbalis in accordance with dying-back type degeneration. The corticospinal tract were affected at 18 weeks,and thalamus、the olfactory pathway、the visal pathway、the auditory pathway were affected at 32 weeks.

这种DS从9周龄开始，首先在构成薄束路和脊髓小脑后路的上行纤维的远端区域-薄束核区和小脑白质出现，逐渐按照延髓、颈髓、胸髓、腰髓的顺序，向细胞体方向逆行性地进展。18周龄时波及到锥体路，32周龄时进一步波及到丘脑和嗅觉、视觉、听觉传导路。

Mitochondria was relatively little in size. Round primary lysosome with high electron-densed granules and secondary lysosome with high or low electron-densed granules were seen frequently. DCs contained many rough endoplasmic reticulum, the Golgi apparatus and ribosomes. The vacuoles with flocculent electron-densed granules were rare. Some special granules in cytoplasm were seen, whose surface like earphone were covered with a membrane. High electron-densed contents in the granules were near one side and the other side was bright. The nucleus became markedly small in volume, nephroid or hoofed in shape. The nucleus had little euchromatin and lots of heterochromatin under nuclear membrane.

子宫内膜癌组织DC超微结构特征如下：细胞形态不规则，与正常子宫内膜组织DC相比，胞膜较光滑，胞膜表面树突状胞浆突起显著减少，部分突起呈粗短状；胞质中线粒体相对少，圆形而电子密度高的初级溶酶体和不规则形且电子密度高低不一的次级溶酶体多见；高尔基体、粗面内质网、核糖体丰富；含微量絮状电子致密物的胞饮小泡显著减少；胞质中可见形态特殊的颗粒，该颗粒外周膜包裹，略呈圆形，中间部位稍弯曲，如耳机状，颗粒中由高电子致密物居于一侧，而另一侧则呈透亮状；胞核显著减小，居于胞质一侧，常呈肾形或马蹄形，核内常染色质较少，异染色质多边集于核膜下。

At G. 32W stable expression of nov mRNA was distributed in pontine abducens nucleus, red nucleus and substantia nigra of midbrain, ventral posterolateral thalamic nucleus and mediodorsal thalamic nucleus.

第32周脑桥展神经核、中脑红核和黑质、丘脑腹外侧核和背内侧核以及第38周大脑纹状体、顶叶皮质显示nov mRNA稳定的表达。

After HRP application to the rostral area (n=10), quite a number of labelled cells were distributed at the ventrolateral part of the nucleus of trapezoid body, the lateroventral periolivary nucleus and the retrotrapezoid nucleus. After HRP application to the caudal area (n=10), HRP labelled cells were mainly located at the lateral reticular nucleus and the ventral part of at the lateral paragigantocellular nucleus LPGi. After HRP application to the intermediate area (n=6), only 8HRP labelled cells were observed at the in 6rats. After HRP application to the control area (n=4), only 6HRP labelled cells were found at the rostral ventrolateral reticular nucleus.

大鼠延髓腹外侧表面的头端化学感受区表面敷贴辣根过氧化物酶后，在延髓斜方体核、外周橄榄腹外侧核和斜方体后核均有HRP标记细胞存在；于尾端化学感受区表面敷贴HRP后，在延髓的巨细胞旁外侧核、外侧网状核和舌下神经核有HRP标记细胞存在；于中间区表面敷贴HRP后，在6只大鼠的延髓内部区域仅观察到8个HRP标记细胞；于对照区敷贴HRP后，在4只大鼠的延髓内部区域仅观察到6个HRP标记细胞，散在地分布在头端腹外侧网状核。

After HRP application, HRP labelled cells were distributed in the nucleus of trapezoid body , lateroventral periolivary nucleus , retrotrapezoid nucleus , lateral paragigantocellular nucleus and lateral reticular nucleus . The findings demonstrated the existence of neural structural relations between the chemosensitve areas and the Tz, LVPO, NRT, LPGi and LRt, and revealed that these nuclei might participate in the mechanism of central chemical regulation on respiration.

观察到用浸有HRP的滤纸局部敷贴于延髓腹外侧表面化学感受区后，在延髓斜方体核、外周橄榄腹外侧核、斜方体后核、巨细胞旁外侧核和外侧网状核等核团找到HRP标记的细胞，表明上述延髓内部核团与腹外侧表面化学感受区之间存在神经结构联系，提示延髓腹外侧表面化学感受区对H〓等物质的化学感受机制可能与上述核团神经元伸向该区的突起有关。

After injection of FG into the NTS, FG retrogradely labeled neurons were present bilaterally with a strong ipsilateral predominance in the hypothalamic regions, such as medial and lateral parvicellular portion of the paraventricular nucleus, posterior

将FG注入孤束核后，在下丘脑室旁核小细胞部、穹窿周区、下丘脑后区及弓状核等核团内都观察到数量较多的FG逆行标记神经元；EM1和EM2阳性神经元的胞体主要位于下丘脑背内侧核、腹内侧核

The major clinical manifestation was lymph node enlargement found in all patients. Skin involvement and osteolysis were not frequent. The characteristic finding was leukemic cells with highly indented or lobulated flower-like nuclei in peripheral blood and bone marrow. ATL cells were CD2,CD3,CD4,CD25 positive and CD8 negative. No specific chromosome abnormality or HLA type was found. Seven of 8 patients examined had HTLV-Ⅰ antibody. The HTLV-Ⅰ provirus genome sequence integrated into host cell DNA was amplified by PCR and confirmed by liquid hybridization. All of these results showed that HTLV-Ⅰ was also the etiological agent of ATL in China. One of the 9 cases of ATL was classified as lymphoma type, one as chronic-type, and the rest as acute type.

结果和结论确诊9例ATL，其临床特征主要为淋巴结肿大，皮损及溶骨改变少见，外周血及骨髓出现典型的花瓣状核淋巴细胞，胞体大，核畸形，免疫表型为CD-1、CD+2、CD3+、CD4+、CD-8，还表达CD25等激活标记，染色体核型及HLA分型未发现有规律性的变化。8例ATL中有7例血清HTLV-Ⅰ抗体阳性，应用PCR及液相杂交法可检出整合到宿主细胞DNA中的HTLV-Ⅰ病毒序列，表明在我国HTLV-Ⅰ也是ATL的病因。9例中1例为淋巴瘤型，1例为慢性型，7例为急性型ATL，治疗效果及预后均不良。

Four weeks later, in the baicalin group, some spindle-shaped UCB-MSCs began to present shrinkage, with slender processes on the cell edge, and some UCB-MSCs tended to be spherical-, conical-, and triangle-shaped appearance, with many slender processes on the pseudopodia.

细胞形态学观察：脐血单个核细胞培养第2~3天开始贴壁生长，2周左右达高峰，3周后细胞生长达80%~90%融合，此时脐血原始间充质干细胞呈较均一的长梭形。4周后，黄芩苷组原来呈梭形的胞体一部分发生收缩，细胞边缘出现细的突起；一部分胞体逐渐近似圆形、锥形、三角形，伪足有多个细长突起。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新