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The result showed that IFN-γ was distributed over 20 nucleus of the hypothalamus, such as nucleus mamillaris medialis, nucleus pre-opticus medialis, area anterior hypothalami, nucleus ventromedialis, nucleus reunions, nucleus paraventricularis, nucleus supra optic, and etc. Many of IFN-γ positive nerve fibers were found in supra optic nucleus, paraventricular nucleus, nucleus mamillaris medialis and etc. Nerve fibers and astrocytes IFN-γ immunoreactive positive cells spreaded in paraventricular nucleus, nucleus pre-opticus lateralis, nucleus pre-opticus medialis and etc. At the same time, IFN-γ is also observed obviously in adenohypophysis and neurohypophysis. IFN-γ also expresses in follicular and corpus luteum.

结果显示,IFN-γ分布于乳头体内侧核、视前内侧核、下丘脑前区、腹内侧核、连接核、室旁核、视上核等20个核团中,各部分核团细胞着色程度深浅不一;在视上核、室旁核分泌部乳头体内侧核等有大量的阳性神经纤维;同时在室周核、视前外侧核、视前内侧核等13个核团中散布有一些阳性星形胶质细胞,与阳性神经细胞交错共存;腺垂体中腺细胞和神经垂体中神经纤维均有IFN-γ表达;卵泡和黄体中也有IFN-γ存在。

The results showed that orexin-like immunoreactiveneurons were observed in the medial preoptic nucleus,periventricular nucleus,paraventricular nucleus,supraoptic nucleus,dorsomedial nucleus,perifornical nucleus,mammillary nucleus,anterior area,lateral area and posterior area of the hypothalamus,with majority in the lateral area,supraoptic nucleus and mammillary nucleus and with minority in t...

结果表明,在猪下丘脑内,开胃素A免疫阳性神经元分布于下丘脑的视前内侧区、室周核、室旁核、视上核、背内侧核、穹隆周核、乳头体核、前区、外侧区和后区等部位,以下丘脑外侧区、乳头体核和视上核出现的免疫阳性神经元最多,以室周核的最少。这一结果与在其他动物上获得的基本相似。

In the forebrain, orexin B immunoreactive neurons mainly localized in the preoptic area,anterior hypothalamic area,periventricular and paraventricular nuclei,posterior hypothalamic area,dorsomedial hypothalamic nucleus,lateral hypothalamic area, perifornical area,zona incerta,mammillary area and so on.

在前脑orexin B免疫阳性神经元胞体主要分布于视前区、下丘脑前区、室旁核、室周核、后区、背内侧核、外侧区、穹隆周核、未定带、乳头体核等部位。

The author could observe the dissolved degeneration of part of sacrospinal muscle fibers,muscle fiber hypertrophy,and a small quantity of inflammatory cell infiltration with a light microscope.

结果]各项酶学检验无异常;组织学光镜下观察到竖脊肌纤维部分溶解变性,肌纤维肥大,少量炎性细胞浸润;电镜下观察到肌纤维灶状溶解,核周线粒体聚集,胞内脂滴、溶酶体增多,肌卫星细胞增殖分化。

Result]All of enzyme tests were normal.The author could observe the dissolved degeneration of part of sacrospinal muscle fibers,muscle fiber hypertrophy,and a small quantity of inflammatory cell infiltration with a light microscope.Focal solution of muscle fiber,the aggregation of mitochondria around the nucleus,the increase of lipid droplet and lysosome in cyte,and the proliferation and differentiation of muscle satellite cell could be observed with an electron microscope.

结果]各项酶学检验无异常;组织学光镜下观察到竖脊肌纤维部分溶解变性,肌纤维肥大,少量炎性细胞浸润;电镜下观察到肌纤维灶状溶解,核周线粒体聚集,胞内脂滴、溶酶体增多,肌卫星细胞增殖分化。

Result]all of enzyme tests were normal.the author could observe the dissolved degeneration of part of sacrospinal muscle fibers,muscle fiber hypertrophy,and a small quantity of inflammatory cell infiltration with a light microscope.focal solution of muscle fiber,the aggregation of mitochondria around the nucleus,the increase of lipid droplet and lysosome in cyte,and the proliferation and differentiation of muscle satellite cell could be observed with an electron microscope.

术中切取竖脊肌标本用于组织病理学观察和透射电镜观察。[结果]各项酶学检验无异常;组织学光镜下观察到竖脊肌纤维部分溶解变性,肌纤维肥大,少量炎性细胞浸润;电镜下观察到肌纤维灶状溶解,核周线粒体聚集,胞内脂滴、溶酶体增多,肌卫星细胞增殖分化。

The author could observe the dissolved degeneration of part of sacrospinal muscle fibers, muscle fiber hypertrophy, and a small quantity of inflammatory cell infiltration with a light, microscope. Focal solution of muscle fiber, the aggregation of mitochondria around the nucleus, the increase of lipid droplet and lysosome in cyle, and the proliferation and differentiation of muscle satellite cell could be observed with an electron microscope. Pathogenesis of chronic lumboscaral compartment syndrome may be as followed.

术中切取竖脊肌标本用于组织病理学观察和透射电镜观察[结果]各项酶学检验无异常;组织学光镜下观察到竖脊肌纤维部分溶解变性,肌纤维肥大,少量炎性细胞浸润;电镜下观察到肌纤维灯状溶解,核周线粒体聚集,胞内脂滴、溶酶体增多,肌卫星细胞增殖分化。

Focal solution of muscle fiber,the aggregation of mitochondria around the nucleus,the increase of lipid droplet and lysosome in cyte,and the proliferation and differentiation of muscle satellite cell could be observed with an electron microscope.

结果]各项酶学检验无异常;组织学光镜下观察到竖脊肌纤维部分溶解变性,肌纤维肥大,少量炎性细胞浸润;电镜下观察到肌纤维灶状溶解,核周线粒体聚集,胞内脂滴、溶酶体增多,肌卫星细胞增殖分化。

When aborted rats in group E were treated with the dose of 50 IU/g IFN-γ, expression of TNF-αin this group was compared with the other four groups, the results were as follows: Compared with group A, the nucleus pre-opticus medialis, nucleus pre-opticus mango celluaris, nucleus periventricularis hypothalami, nucleus ventromedialis hypothalami, nucleus dorsomedialis hypothalami, nucleus arcuatus hypothalami and pars intermedia had a highly expression of TNF-α, in the other nuclei of hypothalamus, neurohypophsis and adenohypophysis, the change was not significantly, the expression of TNF-αin follicle and corpus luteum were decreased distinctly, the variation in uterus was slight and no difference was exsited; Compared with group B, the expression of TNF-αin nucleus nucleus lateralis hypothalami, nucleus ventromedialis hypothalami, nucleus arcuatus hypothalami, adenohypophysis and uterus presented a remarkable enhancement, conversely, the TNF-αexpression in the nucleus pre-opticus medialis, nucleus pre-opticus suprachiasmaticus, nucleus supra-opticus, nucleus pre-opticus mango celluaris, nucleus periventricularis hypothalami, nucleus paraventricularis hypothalami, neurohypophsis, follicle and corpus luteum had been significantly decreased; Compared with group C, the nucleus pre-opticus medialis, nucleus ventromedialis hypothalami, nucleus dorsomedialis hypothalami, nucleus arcuatus hypothalami, adenohypophysis, pars intermedia and uterus had upregulated the expression of TNF-α, however, it was decreased in the nucleus periventricularis hypothalami and corpus luteum; When compared with group D, the expression of TNF-αin nucleus lateralis hypothalami, nucleus dorsomedialis hypothalami, nucleus ventromedialis hypothalami, pituitary, follicle and ovary stroma were obviously enhanced, while in the nucleus supra-opticus and nucleus arcuatus hypothalami, the situation was definitely opposite, in the other parts, the change was not significant.

外源腹腔注射50 IU/g IFN-γ后,同A组相比:E组TNF-α免疫阳性产物在下丘脑视前内侧核、视前大细胞核、室周核、腹内侧核、背内侧核、弓状核、垂体中间部显著增高,在下丘脑其它核团、神经垂体、垂体前叶较A组差异不显著,在卵泡、黄体中阳性产物表达均较A组显著降低,在子宫中表达较A组变化不明显;同B组相比:E组TNF-α免疫阳性产物在下丘脑外侧核、腹内侧核、弓状核、垂体前叶、子宫均显著增高,在视前内侧核、视交叉上核、视上核、视前大细胞核、室周核、室旁核、神经垂体、卵泡、黄体中阳性物质表达均显著降低;同C组相比:E组下丘脑视前内侧核、腹内侧核、背内侧核、弓状核、垂体前叶与中间部、子宫均显著增高,在室周核、黄体中均显著降低;同D组相比:E组下丘脑外侧核、背内侧核、腹内侧核、垂体各部、卵泡、卵巢基质均显著增高,在视上核、弓状核显著降低,在其它部位表达变化差异不显著。

When aborted rats were injected with 10 IU/g IFN-γin group D, compared with group A, expression of TNF-αinmmuopositive substance in the nucleus pre-opticus medialis, nucleus supra-opticus, nucleus pre-opticus mango celluaris, nucleus periventricularis hypothalami, nucleus paraventricularis hypothalami, nucleus arcuatus hypothalami were highly increased, conversely, the ones in the nucleus pre-opticus suprachiasmaticus, neurohypophsis, adenohypophysis, ovary were obviously decreased, the change in the uterus was not remarkable; Compared with group B, expression of TNF-αin the arcuatus hypothalami was obviously enhanced, while in the nucleus pre-opticus suprachiasmaticus, nucleus pre-opticus mango celluaris, nucleus periventricularis hypothalami, nucleus paraventricularis hypothalami, nucleus ventromedialis hypothalami, nucleus dorsomedialis hypothalami, neurohypophsis, pars intermedia, ovary, the results were on the contrary, the uterus had no significantly variation; Compared with group C, expression of TNF-αin the nucleus pre-opticus medialis, nucleus supra-opticus, nucleus paraventricularis hypothalami, nucleus arcuatus hypothalami, adenohypophysis and pars intermedia had an obviously risen trend, however, the variation in the nucleus pre-opticus suprachiasmaticus, nucleus periventricularis hypothalami, neurohypophsis, pars intermedia, follicle and corpus luteum was completely opposite, uterus had no obviously change.

外源腹腔注射10 IU/g IFN-γ后,同A组相比:D组TNF-α免疫阳性产物在下丘脑视前内侧核、视上核、视前大细胞核、室周核、室旁核、弓状核显著增高,在视交叉上核显著降低,在其它核团较A组无显著差异,在神经垂体、垂体前叶、卵巢中阳性产物表达均较A组显著降低,在子宫中的表达较A组变化不明显;同B组相比:D组TNF-α免疫阳性产物在弓状核显著增高,在视交叉上核、视前大细胞核、室周核、室旁核、腹内侧核、背内侧核、神经垂体、垂体中间部、卵巢各部阳性物质表达均显著降低,在子宫表达变化不显著;同C组相比:D组下丘脑视前内侧核、视上核、室旁核、弓状核、垂体前叶与中间部均显著增高,在视交叉上核、室周核、神经垂体、卵泡、黄体中均显著降低,子宫中表达变化不明显。

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