核周体

Cathepsin D and β-tubulin marked with fluorescence were followed in distribution observed by laser scaning microscope, the distribution was from nucleus nearby to cytoplasm and apophysis, which suggested that they took part in traffic in cells.

荧光双标组织蛋白酶D及β-tubulin，共焦激光扫描显微镜下观察，可见二者伴随分布，均由开始的核周分布明显到散在分布于胞质及突起中，说明溶酶体与骨架蛋白密切相关，提示共同参与细胞内的运输。

The result of framework protein and lysosome marked with fluorescence was observed in sym-acorched laser scaning microscope: red fluorescent β-tubulin and green fluorescent eathepsin in nerve cell were seen in distribution in the same sample. The distribution of both was the same, and was located in around nucleus in the early of differentiation, and in cytoplasm and apophysis in the maturity of differentiation.

共焦激光扫描显微镜镜观察荧光双标的骨架蛋白和溶酶体的染色结果：在同一标本上，不同波长的单光激发可分别见到发红色荧光的β-tubulin和发绿色荧光的组织蛋白酶D在神经元内的存在及分布情况，二者的分布区域大致相同，神经元分化初期，较集中分布在核周附近，神经元分化成熟则分布于胞质及突起内。

RESULTS: Fifty minutes after addition of inducing medium, under the optical microscope, BMSCs exhibited a typical morphology of perikaryon. Three hours later, most of cells became into bipolar or multipolar neuron-like cell appearance with cell bodies and processes.

结果：加入诱导液50 min后，光镜下骨髓间充质干细胞呈典型的核周体形态，3 h后大多数细胞转变为双极或多极神经元细胞样形态，出现胞体和突起。

POMC neurons are medium and large sizes, displaying a quadrangular or multipolar perikaryon with a variable number of primary dendrites, well-ramified dendrites.

POMC神经元细胞体较大，成四边形或多极核周体，一级树突数量不定，且分支较多。

One view is that the nucleocapsids get tegument in the nucleus, and by budding into perinuclear space, they obtain their envelope from the inner lamella of the nuclear membra...

一种意见认为，核衣壳在细胞核内获得被膜，并通过出芽在内层核膜处获得其囊膜后，进入核周间隙，进一步经与核周间隙相连接的内质网，以囊泡转运的方式经高尔基体到达细胞膜，囊泡膜与细胞膜融合，病毒颗粒释放到细胞外。

Then we transfected transitorily the recombinant of green fluorescent protein gene and middle molecular weight neurofilament cDNA into wide type N2a (N2a/wt) and N2a/tau40 to observe the effect of tau accumulation on GFP-NFM fusion protein transport in cellular processes in living cells. At last we used an apoptotic inducer, camptothecin (an inhibitor of topoisomerase-1) to treat N2a/wt and N2a/tau40 cell lines, and compared their apoptotic response.

主要结果如下：一、tau蛋白过度表达和聚积对细胞形态的影响：倒置显微镜下观察两种细胞的形态，发现N2a/wt细胞的突起多而长，而N2a/tau40细胞胞体变圆，突起明显缩短；免疫印迹结果显示转染了tau40的细胞内tau的免疫反应约增加14倍，免疫荧光结果显示N2a/tau40细胞胞体内呈现出较强的红色荧光，tau主要分布在核周和突起起始部分的胞质内，而N2a/wt细胞内的荧光很弱。

Activation of lymphocytes and liver regeneration both involve perinuclear clustering of lysosmes.

淋巴细胞的激活以及肝脏的再生都与溶酶体在核周的集聚有关。

The ARFGAP3 is a cytosolic protein and concentrated mainly in the perinuclear region when it is overexpressed in mammalian cells.

ARFGAP1分布于细胞质中，当表达量高时在核周聚集成团块状、颗粒状，并聚集在高尔基体上发挥功能。

While the tissue spaces surrounding a few blood vessels wasAl and Fg positive,no Al or Fg positive cells were observed.In antemortem injurygroup,diffuse subarachnoid hemorrhage,cerebral edema,swelling or pyknotic neu-rons could be observed.The axons showed irregular swelling and disconnection at1～3h,marked swelling and disconnection at 6h,and retraction ball at 15h whichwas more remarkable at 24h after injury.The space between myelin sheaths andaxons was increased at 3～6h after injury.Tortuous and wavelike myelin sheathswhich adhered on axons incompletely,or even peeled off could be found from 15hto 24h after injury.Perinuclear lysis of Nissl bodies began at 24h after injury.Thenumber of GFAP positive cells in cerebrum and brain-stem increased significantlyfollowed by decrease,and then increased again,but the time courses of the changesin different areas of brain were not same.Al and Fg positive neural cells,mainlysurrounded blood vessels,with diffuse or peripherally distributed positive matter incytoplasm could be observed at 0.5h after injury.The number of Al or Fg positivecells and the intensity of immunoreaction increased with the time of injury.The areaof SYN positivity in medulla oblongata and pons decreased notably 3～6h afterinjury,then return to normal levels and continued to 24h after injury.

生前损伤组，可见广泛蛛网膜下腔出血，脑组织水肿，神经细胞肿胀，晚期神经元固缩；伤后1～3h见部分神经轴突不规则增粗、断裂，伤后6h断端膨大，伤后15h可见收缩球，至伤后24h更为明显；伤后3～6h可见部分神经髓鞘与轴突之间的间隙增宽，伤后15h髓鞘明显曲折，不完全附着在轴突两侧，甚至剥脱，持续到伤后24h；核周尼氏体减少在伤后24h才开始出现；同一部位的GFAP阳性细胞数目随损伤时间发生改变，先增多（最早在伤后0.5h），达到高峰后减少，其后又有增多趋势，但不同部位的GFAP阳性细胞数目增减的时间过程不尽相同，同时，大脑中的GFAP阳性细胞数目也有改变；伤后0.5h，可在脑干组织中见到Al和Fg阳性神经细胞，主要位于血管周围，阳性物在胞浆中呈弥散性分布，但部分细胞的阳性物仅分布于靠近胞膜的胞浆中而呈环状，随损伤时间延长，阳性细胞数目增多，反应强度增加；伤后3～6h，延髓及桥脑中的SYN阳性物面积减少，其后恢复到正常水平，并持续到伤后24h。

Results: Compared with that treated with anti-thyroid drugs alone, after the combined therapy, the size of thyroid decreased significantly, P＜0.01. Typical apoptosis appearance, such as vacuolated cell, marginal nuclei, chromatin condensation and nuclear fragmentation, could be seen under light microscopy. The apoptotic rate before and after adding Chinese herbs was (2.11±1.78)%and (18.66±20.01)%respectively (P＜0.01). TUNEL positive cells were detectable for up to 2-20 weeks after Chinese herbal treatment.

结果：与单纯抗甲状腺药物治疗比较，患者经抗甲状腺药物与中药联合应用治疗2-10周后，甲状腺组织明显缩小(P＜0.01)；光镜下可见细胞发泡、核着边、核固缩、核小体、核分裂等凋亡细胞的典型形态；加中药治疗前后甲状腺细胞凋亡比率分别为(2.11±1.78)%和(18.66±20.01)%(P＜0.01)；末端标记呈阳性。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新