核变体

The electron microscope showed increased number of mitochondrion and size of the transfected SGC7901 gastric cancer cells with NHE1 antisense gene, expanded Golgi apparatus, rough endoplasmic reticulum and endoplasmic reticulum and strengthened heteropolarity.

透射电镜下细胞线粒体数量增多、体积增大，并可见髓鞘样变，粗面内质网、高尔基体较发达，内质网扩张，核偏向一侧，极性增强。

In summary, our results suggest that ANE increases micronuclei frequency, induces cell cycle arrest at G2/M phase, causes cytokinesis failure and accumulates hyperploid cells. The events are possibly associated with ROS production, actin filament disorganization and centrosome aberration.

故从本实验的结果，我们认为槟榔子萃取物会增加微核频，诱导细胞周期停滞於G2/M phase，造成细胞质分缺损与多倍体细胞的增多；而这些现象可能与活性氧的生成，actin filament 的常与中心体的变有关。

There are three categories of maturity indicators of organic matter in current petroleum industry to evaluate the degree of thermal evolution for source rocks, including: 1 optical indicators, i.e., vitrinite reflectance, bitumen reflectance, sporopollen color and thermal alteration index, conodont color alteration index, and reflectance of fossil fragments; 2 chemical indicators, i.e., the maximum temperature in pyrolysis of organic matter, methyl phenanthrene indicator, the atomic ratio of H/C, carbon isotope indicator, and biomarker indicators; 3 indicators of spectroscopy, i.e., the free radical concentration of kerogen, the average structural scale of aromatic nucleus of kerogen, and the laser-Raman spectrum of organic carbon.

摘 要：当前常用的有机质成熟度指标包括3大类：1 光学指标，包括镜质体反射率、沥青反射率、孢粉颜色、热变指数(thermal alteration index， TAI)、牙形石色变指数(color alteration index， CAI)和生物碎屑反射率；2 化学指标，包括有机质最大热解峰温度、甲基菲指数(methyl phenanthrene indicator， MPI)、H/C原子比值、碳同位素指标和生物标志物指标；3 谱学指标，包括干酪根的自由基浓度、干酪根芳核平均结构尺寸和有机碳激光拉曼光谱。

In order to study the microscopic mechanism of martensitic nucleation and growth proeesses, molecular dynamics simulation of thermally induced martensitic transformation in stoichiometric NiAl alloy with B2 structure has been perfomied via the embedded atom method interatomic potential of NiAl alloy.

沙宪伟，张修睦，陈魁英，李依依摘要：运用NiAl合金的镶嵌原子势，进行了B2结构NiAl中热诱发马氏体相变的分子动力学模拟，从而研究马氏体形核和长大的微观机理。

The results show that AREEC liquid crystal enables the flow behavior of PC to change from quasi-Newtonian type to pseudo-plastic type, and it can act as heterogeneous nuclei to accelerate greatly crystallization of PET, and then to improve evidently their injection moldability.

将AREEC液晶与PC、PET熔体共混，研究了共混体系的流变行为、结晶特性和可模塑性，证明AREEC液晶能使PC流变行为由准牛顿型变为假塑性幂律型，能对PET起异相成核和显著加速结晶作用，从而大大改善它们的注塑可塑模性。

Fluorescence in situ hybridization was carried out to confirm the integration of HBV DNA into male pronucleus and its replication with cell division in embryonic development.(Reverse transcriptase polymerase chain reaction,RT-PCR) and immunofluoresence assay were performed to observe the expression of the HBV gene in two-cell stage.

材料与方法：成熟雄鼠麻醉后双侧睾丸注射经脂质体DOSPER包裹的HBV质粒，手术后雄鼠与超排雌鼠合笼交配，用荧光原位杂变(Fluorescence in situ hybridization，FISH)分别检测单细胞胚和二细胞胚间期核中HBV DNA的存在与复制，用逆转录聚含酶链反应(Reverse transcriptase-polymerase chain reaction，RT-PCR)和免疫荧光方法检测HBV基因在二细胞胚胎中的表达。

Then we transfected transitorily the recombinant of green fluorescent protein gene and middle molecular weight neurofilament cDNA into wide type N2a (N2a/wt) and N2a/tau40 to observe the effect of tau accumulation on GFP-NFM fusion protein transport in cellular processes in living cells. At last we used an apoptotic inducer, camptothecin (an inhibitor of topoisomerase-1) to treat N2a/wt and N2a/tau40 cell lines, and compared their apoptotic response.

主要结果如下：一、tau蛋白过度表达和聚积对细胞形态的影响：倒置显微镜下观察两种细胞的形态，发现N2a/wt细胞的突起多而长，而N2a/tau40细胞胞体变圆，突起明显缩短；免疫印迹结果显示转染了tau40的细胞内tau的免疫反应约增加14倍，免疫荧光结果显示N2a/tau40细胞胞体内呈现出较强的红色荧光，tau主要分布在核周和突起起始部分的胞质内，而N2a/wt细胞内的荧光很弱。

The correlations among the parameters suggested that larger trophozoite had its shape closer to round but the shape of nucleus changed little.

参数间的相关系数显示，随着滋养体增大，虫体的形态变圆，而核的形态变化不大。

Results Under TEM, the surface and nuclear membrane split, a great number of vacuoles and high electron density particles appeared in the cytoplasm in Pc trophozoites, vacuoles also did in Pc cysts, intracystic bodies became denaturation and necrosis; under SEM, filopodia and microvilli became sparse,short and fell off, a breach appeared in the surface membrane in Pc trophozoites.

结果 透射电镜发现Pc滋养体表膜和核膜破裂，胞质中出现大量空泡和高电子密度颗粒，Pc包囊中也出现空泡，囊内小体变性坏死；扫描电镜发现Pc滋养体丝状伪足和微绒毛稀疏、变短甚至脱落，表膜出现缺损。

The cell vacuolar degeneration, membrane structural necrosis, mitochondrin edema, mitochondrin vacuolar degeneration and lysosome myelinization were found in the endothelial cells.

1胞核内也可见纳米细菌，细胞空泡变，膜性结构类似坏死，部分线粒体水肿，空泡变，溶酶体呈现髓鞘图像。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。