核分裂

The observation showed that sterile plants and fertile plants in the pollen mother cell stage is not very different, when microspore mother cells of male sterile plants enter meiosis period, it could form normal tetrad cell , tetrad cell is normal in early development stage, In the uninucleate microspore stage , tapetal cells were vacuolization, the microspores were extruded to the middle by tapetal cells.

本研究利用石蜡切片法，结合光学显微镜技术对樱桃萝卜核质互作雄性不育系的不育株和对应保持系可育株的花药发育过程进行细胞形态学观察，观察结果表明：不育株与可育株在花粉母细胞时期差别不大，不育株的花药小孢子母细胞进入减数分裂期后，可以正常的形成四分体，四分体前期发育正常，而在单核小孢子期绒毡层细胞出现液泡化，绒毡层细胞向中间挤压小孢子。

Observation on the ultrastructure of the radicle cell of cabbage seeds indicated that the ultrastructure changed with the seed vigor reduced, such as the segregation of cytoplasm from the cell wall, the fragmentation of cell membrane, the vesiculation of protoplasm, the homogenization of chromation, the disintegration of cytoplasm, the distinguishing of nuclear membrane and nucleous ,and so on.

胚根细胞亚显微结构电镜观察结果，甘蓝种子通过人工老化活力降低后，胚根超微结构发生了变化，主要表现为：质壁分裂，细胞膜内陷或局部断裂，细胞质空泡化，细胞器难以辨认，染色质均质化，核仁和核膜的界限模糊等现象。

RESULT: 1.Ouabain act on lens sodium-pump,under the LM,lens anterior capsular membrane discontinuous,epithelium cells clustered,occluding zonule seperated,lens fiber layers fractured.Under the EM,cells totally hollowed,mitochondria swelling,myelin figure appeared.RT-PCR examine the expression condition of αsubunit of sodium pump on mRNA level,α1、α2 and α3-isoform are all decreased.2.Digoxin act on lens sodium-pump,under the LM,lens cell oedema,linkage distructed,extensive exfoliation.Under the EM,plasma appeared little half-transparant hollow region,mitochondria swelling and ridge disappeared. RT-PCR examine,α1、α2 and α3-isoform are all decreased.3.Amphotericin B act on lens sodium-pump,under the LM,lens epithelium cells linked tightly,arranged in-line,lens fiber layers arranged tightly and regularily.Under the EM,abbundant cellular organes,exuberant cells function indicated. RT-PCR examine the expression condition of αsubunit of sodium pump on mRNA level,α1 and α3-isoform are increased significantly,demonstrated isoform-specific action.4D-thyroxine act on lens sodium-pump,under the LM,lens plasmalemma integrated,cells arranged tightly and regularily.Under the EM,nucleus fission appeared,desmosome half-desmosome and tensile microfilaments linked the cells. RT-PCR examine,α2 and α3-isoform are increased, also demonstrated isoform-specific action.5.Vitamin E act on lens sodium-pump,under the LM,lens anterior capsular membrane continuous and smooth,epithelium cells tightly linked,lens fiber layers appearede hollow region occasionally.Under the EM,lateral membrane high density belt appeared,abundant nucleolus. RT-PCR examine,onlyα1-isoform are increased, demonstrated significantly isoform-specific action.6.DMSO act on lens sodium-pump,under the LM,lens anterior capsular membrane slightly thicker,cells linkage partly distructed.Under the EM,plasmalemma denaturation,mitochondria swelling.RT-PCR examine,α1、α2 and α3-isoform are all altered slightly and haven't significant meanning.

结果：1、哇巴因作用于晶状体钠泵后，光镜下晶状体前囊膜断裂、上皮细胞聚积、闭合连接分离、纤维板层裂隙，电镜下全层细胞空泡化、线粒体肿胀出现髓样结构，RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达均减弱。2、地高辛作用于晶状体钠泵后，光镜下晶状体细胞水肿、细胞连接破坏、广泛剥离，电镜下胞质见少许半透明空化区、线粒体肿胀嵴消失，RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达均减弱。3、两性霉素B作用于晶状体钠泵后，光镜下晶状体上皮细胞紧密连接、线状排列、纤维板层紧密规整，电镜下细胞器丰富、细胞功能旺盛，RT-PCR法检测α1及α3表达显著增强、具有一定的重整异构作用特异性。4、D甲状腺素作用于晶状体钠泵后，光镜下晶状体质膜完整、细胞排列紧密规整，电镜下胞核见分裂像、细胞间有桥粒、半桥粒及张力微丝，RT-PCR法检测α2及α3表达均增强、亦有一定的重整异构作用特异性。5、维生素E作用于晶状体钠泵后，光镜下晶状体前囊膜连续光滑、上皮细胞紧密连接、纤维板层偶见空化，电镜下囊侧膜内有高电子密度带、细胞核仁丰富，RT-PCR法检测仅有α1的表达显著增强、具有极强的重整异构作用特异性。6、二甲基亚砜作用于晶状体钠泵后，光镜下晶状体前囊膜轻度增厚、细胞连接部分破坏，电镜下质膜变性、线粒体肿胀，RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达无显著改变。

In prokaryotes the nuclear material is simply a circular strand of DNA, not complexed with histone proteins, and cell division is amitotic.

在原核生物中，核物质仅是一个简单的环状 DNA ，而不与组蛋白结合，并且细胞为无丝分裂。

Genetic diversity in 12 populations of apomictic Eulaliopsis binata was evaluated by morphological observation and RAPD analyse ,as a result, 12 populations of E.binata were divided into different types.

通过分析龙须草核型和观察花粉母细胞减数分裂过程中染色体行为与变异，判断其倍性水平和多倍体类型。

Then all flower buds develop into binucleate stage and keep to the next year until the flower bloom.

当花序生长停止，则进入长约一个月之单核末期与有丝分裂期，然后再进入双核期。

Centrosome A structure found in all eukaryotic cells, except fungi, that forms the SPINDLE during cell division.

中心体：在除真菌以外的所有真核细胞中存在一种结构，在细胞分裂时可以形成纺锤体。

Centrins play a fundamental role in centrosome duplication and contraction of centrin-based fiber systems in eukaryotic cells.

中心蛋白是真核细胞中一种重要的蛋白质，与细胞中纤维的收缩、细胞的分裂等密切相关。

Genotoxicity of ethylicin and bismerthiazol on Vicia faba root tip cells was studied.

利用蚕豆根尖微核技术研究农药乙蒜素和细菌溴腈对蚕豆根尖细胞的遗传损伤，测定蚕豆根尖细胞的微核率、有丝分裂指数和染色体畸变率。

Generative nuclei The two gametic nuclei formed by the division of the generative cell in the pollen tube of angiosperms.

雄原核：在被子植物花粉管中的生殖细胞分裂成两个配子的核。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。