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Objective To explore the diagnostic value of anti-nucleosome antibody in systemic lupus erythematosus.

目的探讨抗核小体抗体在系统性红斑狼疮中的实验诊断价值。

In prokaryotes the hereditary material is found in the nucleoid.

在原核生物遗传性材料在类核小体被找到。

AIM: To construct the eukaryotic expressing vector of human cbl and test its expression in African green monkey kidney cell line COS 7. METHODS: Human cbl gene tagged with flag was amplified from pEFHAcbl plasmid by PCR. The product was cloned into pGEM T easy, sequenced and then subcloned into eukaryotic expressing vector pcDNA3.1.

以含有人全长cbl cDNA的质粒pEFHAcbl 为模板,采用PCR方法扩增cbl基因,并在其N 末端带上含24 bp的flag标签,克隆到pGEM T easy载体并测序,再亚克隆至真核表达载体pcDNA3 1,酶切鉴定正确后采用脂质体法瞬时转染COS 7细胞,Western blot检测flag cbl在细胞中的表达。

METHODS: Human cbl gene tagged with flag was amplified from pEFHAcbl plasmid by PCR. The product was cAIM: To construct the eukaryotic expressing vector of human cbl and test its expression in African green monkey kidney cell line COS7. METHODS: Human cbl gene tagged with flag was amplified from pEFHAcbl plasmid by PCR. The product was cloned into pGEMT easy, sequenced and then subcloned into eukaryotic expressing vector pcDNA3.1.

以含有人全长cbl cDNA的质粒pEFHAcbl 为模板,采用PCR方法扩增cbl基因,并在其N末端带上含24 bp的flag标签,克隆到pGEMT easy载体并测序,再亚克隆至真核表达载体pcDNA31,酶切鉴定正确后采用脂质体法瞬时转染COS7细胞,Western blot检测flagcbl在细胞中的表达。

Retinotopy; color sensitivity; color-selective region; lateral geniculate nucleus; visual cortex; area V8; area V1; cones; visual pigments; fovea centralis; macula lutea; red; green; blue; Young-Helmholtz theory; short-wavelength-sensitive cones; medium-wavelength-sensitive cones; long-wavelength-sensitive cones; S cones; M cones; L cones; visual perception; ectostriatum; tectofugal visual pathways; thalamofugal visual pathways; color-reversal learning; visual Wulst

retinotopy;感色灵敏度;颜色-选择区;侧膝体核;直观皮层;区域V8;区域V1;圆锥细胞;视色素中央凹;黄斑;红色;绿色;蓝色;三原色学说;短波长敏感圆锥细胞;中波长敏感圆锥细胞;长波长敏感圆锥细胞;S圆锥细胞;M圆锥细胞;L圆锥细胞;视觉;ectostriatum;tectofugal视路;thalamofugal视路;颜色-倒序学习;visual Wulst

Objective To investigate the effects of subthalamic nucleus -deep brain stimulation on expression of dopamine and adenosine 3'5'-monophosphate-regulated phosphor-protein (DARPP-32) and its phosphorylated proteins in corpus striatum of rat models with levodopa-induced dyskinesia.

目的 探讨丘脑底核高频电刺激对帕金森病异动症大鼠纹状体区多巴胺和cAMP调节的磷蛋白(DARPP-32)及其磷酸化蛋白表达的影响。

Four weeks later, 69 Parkinson disease model s were randomly divided into 3 groups:①Control group(n=24):4.5 μL normal saline was injected into head of caudate nucleus of right-side corpus striatum.

取SD雄性大鼠,右侧中脑黑质致密区注入6-羟基多巴(2g/L)3μL制备帕金森病模型,4周后将69只成功帕金森病模型随机分为3组:①对照组24只:右侧纹状体尾状核头部注入生理盐水4.5μL。

Objective To investigate the gene expression of Nuclear factor-κB in the prefrontal cortex, hippocampus and corpus striatum in MK-801 induced hypoglutamatergic schizophrenia model rats to explore the molecular mechanism of schizophrenia.

目的 了解地卓西平马来酸盐[Dizocilpine maleate, MK-801]所致谷氨酸能低下精神分裂症大鼠前额皮质、海马、纹状体核因子-κB(Nuclear factor-κB, NF-κB)基因的表达,探讨其参与精神分裂症的病理机制。

METHODS:① Model group: The rats were made into models of Parkinson disease with damaged corpus striatum by injecting 6-hydroxydopamine (6-OHDA, 12 μg), which was dissolved in 5 μL normal saline containing 2 g/L ascorbic acid, slowly into caudal putamen.

①模型组:将6-羟基多巴(12 μg溶于5μL含2 g/L抗坏血酸的生理盐水中)缓慢注入尾壳核,建立纹状体毁损帕金森病大鼠模型,不干预。

A distincitve feature of these blasts is a linear red "Auer rod" composed of crystallized granules.

非常大的、多核的、未成熟的髓母细胞,这些细胞中可见典型的线性红色Auer小体。

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