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In the study, a set of near isogenic lines of CMS-S, S-Mo17Rf3Rf3 and S-Mo17rf3rf3, were adopted, and TdT-mediated dUTP nick-end labeling, in situ immunolocalization of cytochrome c and DNA ladder assay were employed to study the programmed cell death process on cytology and DNA level. The microscope observation evidenced that tapetal cells of fertile anthers kept intact until microspores, and then were disintegrated on later developmental stage.

S型细胞质雄性不育系的一对近等基因系S-Mo17Rf3Rf3 和S-Mo17rf3rf3为材料,采用TdT介导的dUTP DNA末端标记、细胞色素C免疫原位杂交和DNA寡聚核小体片段电泳等方法,分别在细胞学水平和DNA水平上研究了玉米CMS-S小孢子败育的细胞程序性死亡过程。

We have studiedthe coordination water-substituted reactions and the phase-transferchemistry of dozens of trinuclear or tetranuclear sandwich-typePOMs and found that organic molecules or polymers can becoordinated to these polyanions after dehydration, thus more thanone hundred coordinated products are obtained, indicating that thesepolyanions are excellent ligands in organic solvents.

我们首次对夹心三、四核等几十种化合物中配位水进行了配位水取代反应及相转移化学研究,脱水后可配位有机分子或有机高分子,得到上百种配位产物,因此在有机溶剂中它们是优秀的配体。

The ventral partition of medial geniculate body has been known as the special nucleus of relaying auditory information. In auditory processing, a primary function of thalamocortical relay neurons in MGBv is to encode sound signals as different sequences of action potential.

内侧膝状体腹侧部(ventral partition of medial geniculate body,MGBv)是特异的听觉中继核,在成年哺乳动物听觉处理过程中,位于MGBv的丘脑-皮层神经元的主要功能是以不同的动作电位顺序编码声音信号。

To understand the basic properties, underlying mechanisms and functions of the short-term synaptic plasticity of thalamocortical visual pathway, we combined in vivo field potential recording, focal drug application technique and neuron models, to explore the short-tem synaptic plasticity of rat major thalamocortical visual pathway (from lateral geniculate nucleus to primary visual cortex) and extrageniculate pathway (from lateral posterior nucleus to primary visual cortex), as well as the role of short-term depression in the neuronal correlated firing detection.

我们结合在位场电位记录、局部加药和神经模型等方法,对不同刺激模式下大鼠丘脑皮层主要视觉通路(外侧膝状体到初级视皮层)和非主要视觉通路(膝体外通路:丘脑侧后核到初级视皮层)的短时程突触可塑性,以及短时程抑制作用对神经元同步活动检测的作用做了研究,试图了解视觉丘脑皮层突触传递短时程可塑性的基本特性、神经机制和功能。

During the past 3 years, 2 independent experiments of sperm-mediated gene transfer in domestic silkworms were carried out respectively in 2000 and 2002 with the method direct injection of copulation thecae.

在同源重组绿色荧光蛋白表达载体pG350的基础上,以pMD18-T为质粒载体,用egfp基因替换gfp基因,构建了家蚕核型多角体病毒早期即刻蛋白基因启动子BmNPV-IE控制下的增强型绿色荧光蛋白表达载体pMD-Fib-IE-EGFP。

Methods In order to estimate core temperature T(subscript c from skin temperature T(subscript s, the paper simulated an environmental temperature T(subscript e change. From the change, the paper obtained the thermoresistance ratio of adiabator R(subscript e and body surface tissue R(subscript c, at the same time, deducted environmental temperature T(subscript e influences from the skin temperature T(subscript s. The feasibility and accuracy of the method was tested by a self-made body temperature simulator.

提出通过人为改变环境温度T(下标 e而获得个性化的绝热材料热阻R(下标 e对体表组织热阻R(下标 c比值的途径,实现从皮肤温度T(下标 s中扣除环境温度T(下标 e影响,从而达到从皮肤温度T(下标 s测值中估计体核温度T(下标 c的方案和具体测量方法,并用自行设计的模拟体温发生器对此方案进行了可行性和准确性验证。

With two tripodal ligands, dtma and tmpa, dtma=diethylenetriamine-N'-acetate and tmpa=tris(2-pyridyl-methylamine and a polyamine ligand, trien (=triethylenetetramine), serving as terminal ligands, we synthesized 19 species of new imidazolate -bridged Cu-Zn, Zn-Zn and Cu-Cu, and other relating compounds, in which 〓 and 〓 complexes are model compounds of Cu, Zn-SOD.

用不等臂三脚架dtma(diethylenetriamine-N'-acetate)、直链trien和等臂三脚架tmpatris(2-pyridyl-methyl-amine为配体,合成了一系列咪唑桥联Cu-Zn、Zn-Zn和Cu-Cu等有关19个新化合物,其中两个异双核〓Cu〓和〓〓配合物为Cu,Zn-SOD活性中心的模型化合物。2。

Suprachiasmatic nucleus; Synapse ; Subjunctional body; 5-HT ; Degeneration ; Ultrastructure ; Immunoelectromicroscope ; Tupaia belangeri chinensis

视交叉上核;突触节下小体; 5-羟色胺;溃变;超微结构;免疫电镜;树鼠句

The effect of food concentration on characteristics in life history of Brachionus urceus, such as the duration of principal development stage, net reproductive rate and mixis rate in all the offspring, was studied by the method of clonal culture.

方法]采用单个体培养法,研究了不同浓度的蛋白核小球藻对壶状臂尾轮虫主要发育阶段历时、净生殖率以及全部后代的混交雌体百分率等生活史特征的影响。

Simultaneously the disappearance of microvilli, vacuolization of cytoplasm, dispersion of nucleus and appearance of apoptotic body were observed under transmission electron microscopy. All these morphological changes are hallmarks of apoptosis.

凋亡细胞经荧光染色后表现为细胞核固缩、碎裂;透射电镜下观察发现细胞微绒毛丧失,胞内出现空泡,胞核解体及形成凋亡小体等典型的凋亡特征。

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This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。

There is no differences of cell proliferation vitality between labeled and unlabeled NSCs.

双标记神经干细胞的增殖、分化活力与未标记神经干细胞相比无改变。