核体

The fracture mechanism of totally austenite TWIP steel (steel 5#) is: the moving of dislocations is blocked by twin boundary during deformation. Micro-voids nucleate at boundary of deformation twins by the interaction between deformation twins and dislocations. Micro-voids are formation, growth, coalescence and break by the stress concentration during deformation.

全奥氏体的TWIP钢（如5＃钢）的断裂机制为：形变过程中位错的运动受孪晶界的阻碍，形变孪晶与位错的交互作用使微孔形核于孪晶界处，应力集中使微孔长大、聚合直至材料发生断裂。

The fracture mechanism of the whole austenite TWIP steel (steel 5#) was that the moving of dislocations was blocked by twin boundary during deformation, the microvoids nucleated at boundary of deformed twins by the interaction between deformed twins and dislocations, the microvoids grew, got together and broke by the stress concentration during deformation.

全奥氏体的TWIP钢（2＃钢）的断裂机制为：形变过程中位错的运动受孪晶界的阻碍，形变孪晶与位错的交互作用使微孔形核于孪晶界处，应力集中使微孔长大、聚合直至材料发生断裂。

The fracture mechanism of TWIP steel (steel1#) which contained second phase was that the microvoids nucleated due to the decreasing of binding force between austenite and second-phase or the second phase dehiscence caused by strain hardening, then the microvoids grew, got together and broke by the stress concentration during deformation.

含有第二相的TWIP钢（1＃钢）的断裂机制为：第二相和奥氏体相界面聚合力的减弱或第二相本身加工硬化导致的开裂促使微孔形核，形变过程中产生的应力集中使微孔长大、聚合直至发生断裂。

The fracture mechanism of TWIP steel (steel1#) which contains second-phase is: micro-voids nucleate due to binding force weaken between austenite and second-phase or second-phase dehiscence itself caused by strain-hardening. Micro-voids are formation, growth, coalescence and break by the stress concentration during deformation.

含有第二相的TWIP钢（如1＃钢）的断裂机制为：第二相和奥氏体相界面聚合力的减弱或第二相本身加工硬化导致的开裂促使微孔形核，形变过程中产生的应力集中使微孔长大、聚合直至发生断裂。

Methods The whole-length X gene was directly cloned to pcDNA3.1 vector. The recombinant vector (pcDNA3.1-X) was transfected into HEPG2 cells after selection with hygromycin, steady clones (HEPG2-X cells) were obtained. The expression of RhoC protein was analysed with immunohistochemical stain.

用定向克隆的方法构建X基因的真核表达载体pcDNA3.1-X，脂质体转染HEPG2细胞；潮霉素选择培养稳定表达X基因的HEPG2-X细胞；免疫组化鉴定HEPG2-X细胞RhoC蛋白表达。

At zygotene,t he SC formation initiated at multiple sites in each bivalent but the regions nea r telomeres were preferred.SCs were shortened with development of SCs.Initiation of new SC and extension of existing SC occured simultaneously,and pairing in di fferent bivalents of a nucleus was not synchronised.

结果表明，小麦SC以多点式起始方式于偶线期开始形成；随SC的发育，新的SC形成和已有SC片断的延伸并存；此外，在同一核内不同二价体之间，染色体配对和SC形成并不同步；SC成熟于粗线期，而以破碎方式解体，消失于双线期。

In hypothalamic median eminance of kidney Yang deficiency group, the bounds of ependymocytes were dim, distribution of their microvillus changed, the top of microvillus enlarged and became spherical, and holes occurred on the surface of the cells. In that of control group, the cell arranged in order and the cell bouns were clear, the microvillus were equal in thickness and in long, thin acerose thread wool shape. The surface ultrastructure of ependymocytes on hypothalamic median eminance in herb group was similar to that of control group.

肾阳虚大鼠垂体内分泌细胞和甲状腺细胞存在着不同程度的粗面内质网、高尔基体扩张，线粒体空化，细胞变形，核固缩等超微结构的损伤；中药组大鼠垂体超微结构与正常组类似，细胞形态完整，结构清晰，细胞器结构正常，含有各种大小不等、形态不一的分泌颗粒，但甲状腺细胞超微结构与肾阳虚组相比未见明显改善。

After thecomplete genome extraction of the strain was performed, the genomic DNA was partiallydigested by restriction enzyme Sau3AⅠ, the DNA fragments from 1 to 5Kb was clonedinto prokaryote expression vector pET-28a-c, and transformed host bacteria. The resultsshowed that we succeeded in constructing the gene expression library of haemophilusparasuis serovar 5, which is fundamental for the study of advanced gene screening. Inaddition, primer design was performed based on haemophilus influenzae in this study. In addition, PCR was performed by using genomic DNA of haemophilus parasuisserovar 5 as the template. The results demonstrated that we obtained two neo-gene:23SrRNA gene(conserved gene belonging to the large-subunit of ribosome) and adenylatecyclase gene(encodes adenylate cyclase and participates in converting adenyl nucleosidetriphosphate to cyclic adenosine3",5"-monophosphate). Furthermore, the phylogeneticanalyses between the species was performed, and neighbor-joining tree was constructedbased on comparison of 23S rRNA gene sequences, so it was illuminated betweenHaemophilus parasuis and other species in molecular evolution relationship.

选择我国流行优势菌株副猪嗜血杆菌血清5型地方株为研究对象，提取细菌基因组DNA，用限制性内切酶Sau3AⅠ对基因组DNA进行部分酶切，回收大小为1～5Kb的DNA片段，将其连接入原核表达载体pET-28a-c，最后转化宿主菌，结果成功地构建了基因表达文库，为后续的基因筛选工作奠定基础；另外，本研究选择嗜血杆菌属的流感嗜血杆菌为参考对象进行引物的设计，以副猪嗜血杆菌血清5型地方菌株的基因组DNA为模板，进行PCR扩增反应，结果表明成功地获得两个新基因：23S rRNA基因(存在于核糖体大亚基中的保守性基因)和腺苷酸环化酶基因(负责将腺嘌呤核苷三磷酸转变为环腺苷酸)，并进一步做了不同物种之间的分子系统发育分析，构建了基于23S rRNA基因的邻接法系统发育树，阐明了副猪嗜血杆菌与其它菌种的分子进化关系。

Results of HE dye after cerebral alchemic reperfusion showed: Cortex was infiltrated by neutrophil; Cells of corpus striatum and cortex of parietal lobe were stained light; the number off cells obviously reduced; the feature of cells necrosis, such as cells swelling, nuclear fragmentation and lysis et al, would be observed.

HE染色结果显示：脑缺血再灌注后，皮质区中性白细胞浸润，纹状体外侧和顶叶皮质细胞染色淡，细胞数明显 NF－。B在脑缺血再灌注后脑组织局部炎症反应中的作用减少，出现细胞肿胀，胞核碎裂和溶解等细胞坏死特征。

The channel for producing COOH-+H2,contributed from both aldehydic hydrogen abstraction and nucleophilic addition,is the most favorable channel.

通过计算提出亲核加成过程的反应通道，主要产物生成H2和生成COOH-/HCOO-/OCHO-异构体。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。